Metal accumulation characteristics and physiological response of Kummerowia striata to manganese stress

doi:10.11686/cyxb2020259

Abstract

Abstract:

This research studied the tolerance mechanism of plants to manganese stress. Individual plants of Kummerowia striata were collected from localities contaminated or not contaminated by heavy metals and cultivated under different concentrations of manganese ［0 （Control）， 1000， 5000， 10000， 15000， 20000 μmol·L^-1］. The effects of manganese stress on the phenotype， physiological and biochemical features， and manganese accumulation characteristics of K. striata from these two sources were evaluated. It was found that， as the concentration of Mn increased： 1） The root dry weight， shoot dry weight， and root∶shoot ratio of K. striata from both localities decreased； when the concentration of Mn was in the range 5000-20000 μmol·L^-1， compared with the Control， the shoot and root dry weight of plants from the contaminated locality decreased by 4.34%-27.71% and 19.00%-66.06%， respectively； while the shoot and root dry weight for plants from the uncontaminated locality decreased by 16.33%-49.77% and 27.90%-77.54%， respectively. 2） The contents of chlorophyll， soluble sugar and proline and the activities of superoxide dismutase and peroxidase in leaves of K. striata from the two localities showed a pattern of initial increase and then decrease with increasing Mn exposure， while the content of soluble protein gradually decreased and the content of malondialdehyde gradually increased. 3） The Mn content of the roots， stems， and leaves of K. striata from both localities increased. When grown at the Mn concentration of 20000 μmol·L^-1， the Mn content of K. striata sourced from the contaminated and uncontaminated areas was 16.53 and 13.41 times than that of the Control， respectively. Therefore， the Mn accumulation capacity and Mn tolerance of the K. striata from the contaminated locality are higher than in plants sourced from the uncontaminated locality. High activities of antioxidant enzymes appear to be an important physiological mechanism of K. striata tolerance to high Mn stress.

Key words: Mn stress, heavy metal contaminated area, Kummerowia striata, physiological and biochemical features, accumulation characteristics

Xin-hang LI, Ze-hua XIAO, Xue-shao KUANG, Wu-min WANG, Liang-yu LUO, Wen-sheng LIU. Metal accumulation characteristics and physiological response of Kummerowia striata to manganese stress[J]. Acta Prataculturae Sinica, 2021, 30(7): 139-147.

Figures/Tables 5

References 25

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浓度 Concentration (μmol·L^-1)	芽长 Shoot length (cm)		根长 Root length (cm)		芽干重Shoot dry weight (mg·plant^-1)		根干重Root dry weight (mg·plant^-1)		根冠比 Root-shoot ratio
浓度 Concentration (μmol·L^-1)	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC
0	11.10±0.46b	10.90±0.53b	9.53±0.61a	10.17±1.53a	6.22±0.28ab	6.41±0.39a	4.42±0.19a	4.23±0.11a	0.71±0.03a	0.66±0.03a
1000	12.80±1.11a	12.37±1.03a	9.73±0.78a	9.60±0.66ab	6.77±0.23a	6.79±0.25a	3.88±0.25b	3.71±0.28b	0.57±0.03bc	0.55±0.04bc
5000	11.10±1.12b	9.07±0.87c	9.03±0.55ab	8.40±0.26bc	5.95±0.25b	5.36±0.57b	3.58±0.25b*	3.05±0.07c	0.60±0.07b	0.57±0.06b
10000	9.73±0.87bc	9.33±0.74c	8.16±0.42bc	7.20±0.44cd	5.92±0.46b	5.42±0.22b	3.15±0.17c*	2.66±0.11d	0.53±0.01c	0.49±0.03c
15000	8.93±0.60cd	8.26±0.49c	7.70±0.26c*	6.77±0.25de	5.08±0.33c	4.74±0.57b	2.24±0.10d*	1.89±0.04e	0.44±0.02d	0.40±0.04d
20000	8.13±0.45d*	6.97±0.25d	7.23±0.64c*	5.87±0.42e	4.50±0.41c*	3.22±0.22c	1.50±0.10e*	0.95±0.09f	0.33±0.02e*	0.29±0.01e

浓度 Concentration (μmol·L^-1)	芽长 Shoot length (cm)		根长 Root length (cm)		芽干重Shoot dry weight (mg·plant^-1)		根干重Root dry weight (mg·plant^-1)		根冠比 Root-shoot ratio
浓度 Concentration (μmol·L^-1)	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC
0	11.10±0.46b	10.90±0.53b	9.53±0.61a	10.17±1.53a	6.22±0.28ab	6.41±0.39a	4.42±0.19a	4.23±0.11a	0.71±0.03a	0.66±0.03a
1000	12.80±1.11a	12.37±1.03a	9.73±0.78a	9.60±0.66ab	6.77±0.23a	6.79±0.25a	3.88±0.25b	3.71±0.28b	0.57±0.03bc	0.55±0.04bc
5000	11.10±1.12b	9.07±0.87c	9.03±0.55ab	8.40±0.26bc	5.95±0.25b	5.36±0.57b	3.58±0.25b*	3.05±0.07c	0.60±0.07b	0.57±0.06b
10000	9.73±0.87bc	9.33±0.74c	8.16±0.42bc	7.20±0.44cd	5.92±0.46b	5.42±0.22b	3.15±0.17c*	2.66±0.11d	0.53±0.01c	0.49±0.03c
15000	8.93±0.60cd	8.26±0.49c	7.70±0.26c*	6.77±0.25de	5.08±0.33c	4.74±0.57b	2.24±0.10d*	1.89±0.04e	0.44±0.02d	0.40±0.04d
20000	8.13±0.45d*	6.97±0.25d	7.23±0.64c*	5.87±0.42e	4.50±0.41c*	3.22±0.22c	1.50±0.10e*	0.95±0.09f	0.33±0.02e*	0.29±0.01e

浓度 Concentration (μmol·L^-1)	根 Root (mg·kg^-1)		茎 Stem (mg·kg^-1)		叶 Leaf (mg·kg^-1)		转运系数 Translocation factor
浓度 Concentration (μmol·L^-1)	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC
0	334.71±16.43f*	292.73±13.75f	116.66±26.50f	119.62±14.86f	476.15±16.29f*	391.24±42.56f	0.89±0.04b	0.87±0.07bc
1000	1581.55±88.75e	1389.40±107.66e	544.88±39.18e	480.67±18.33e	2365.99±161.51e*	1776.34±119.95e	0.92±0.01b	0.82±0.11c
5000	4064.93±140.83d*	3296.71±254.86d	2300.77±116.86d	2119.86±198.03d	5024.65±53.59d*	4237.83±181.41d	0.90±0.01b	0.97±0.04ab
10000	6608.24±304.58c*	5005.96±375.29c	5255.48±318.26c*	4646.92±75.34c	8205.72±86.97c*	5442.01±231.46c	1.02±0.02a	1.01±0.06a
15000	8111.45±196.20b*	6265.26±230.93b	6641.18±269.96b*	5734.77±373.44b	10304.69±701.23b*	7479.84±457.84b	1.05±0.06a	1.06±0.09a
20000	9769.83±279.88a*	7588.46±363.03a	7886.09±195.24a*	6740.52±306.26a	12553.03±300.07a*	8558.05±280.97a	1.05±0.06a	1.01±0.03a

浓度 Concentration (μmol·L^-1)	根 Root (mg·kg^-1)		茎 Stem (mg·kg^-1)		叶 Leaf (mg·kg^-1)		转运系数 Translocation factor
浓度 Concentration (μmol·L^-1)	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC	污染区 C	非污染区 NC
0	334.71±16.43f*	292.73±13.75f	116.66±26.50f	119.62±14.86f	476.15±16.29f*	391.24±42.56f	0.89±0.04b	0.87±0.07bc
1000	1581.55±88.75e	1389.40±107.66e	544.88±39.18e	480.67±18.33e	2365.99±161.51e*	1776.34±119.95e	0.92±0.01b	0.82±0.11c
5000	4064.93±140.83d*	3296.71±254.86d	2300.77±116.86d	2119.86±198.03d	5024.65±53.59d*	4237.83±181.41d	0.90±0.01b	0.97±0.04ab
10000	6608.24±304.58c*	5005.96±375.29c	5255.48±318.26c*	4646.92±75.34c	8205.72±86.97c*	5442.01±231.46c	1.02±0.02a	1.01±0.06a
15000	8111.45±196.20b*	6265.26±230.93b	6641.18±269.96b*	5734.77±373.44b	10304.69±701.23b*	7479.84±457.84b	1.05±0.06a	1.06±0.09a
20000	9769.83±279.88a*	7588.46±363.03a	7886.09±195.24a*	6740.52±306.26a	12553.03±300.07a*	8558.05±280.97a	1.05±0.06a	1.01±0.03a

来源 Source	项目 Items	芽干重Shoot dry weight	根干重Root-dry weight	根冠比 Root -shoot ratio	脯氨酸 Proline	可溶性蛋白 Soluble protein	可溶性糖 Soluble sugar	过氧化物酶 Peroxidase	超氧化物歧化酶 Superoxide dismutase	丙二醛 Malonaldehyde	根锰含量 Root manganese content	茎锰含量 Stem manganese content	叶锰含量 Leaf manganese content	来源 Source
非污染区 Non contaminated zone	芽干重Shoot dry weight		0.877**	0.725**	0.627**	0.854**	0.550*	0.695**	0.590**	-0.825**	-0.847**	-0.854**	-0.841**	污染区 Contaminated zone
	根干重Root dry weight	0.921**		0.964**	0.593**	0.950**	0.505*	0.659**	0.572*	-0.940**	-0.954**	-0.948**	-0.962**
	根冠比Root-shoot ratio	0.762**	0.943**		0.560**	0.913**	0.478*	0.606**	0.553*	-0.910**	-0.899**	-0.889**	-0.916**
	脯氨酸Proline	0.806**	0.859**	0.851**		0.638**	0.958**	0.942**	0.960**	-0.540*	-0.466	-0.536*	-0.484*
	可溶性蛋白Soluble protein	0.903**	0.974**	0.914**	0.822**		0.565*	0.722**	0.626**	-0.933**	-0.944**	-0.947**	-0.949**
	可溶性糖Soluble sugar	0.673**	0.714**	0.745**	0.947**	0.684**		0.942**	0.960**	-0.514*	-0.412	-0.496*	-0.426
	过氧化物酶Peroxidase	0.804**	0.816**	0.741**	0.872**	0.843**	0.794**		0.924**	-0.668**	-0.607**	-0.684**	-0.620**
	超氧化物歧化酶Superoxide dismutase	0.829**	0.893**	0.898**	0.971**	0.875**	0.917**	0.884**		-0.554*	-0.456	-0.527*	-0.484*
	丙二醛Malonaldehyde	-0.908**	-0.971**	-0.920**	-0.858**	-0.978**	-0.739**	-0.856**	-0.906**		0.948**	0.952**	0.955**
	根锰含量Root manganese content	-0.883**	-0.976**	-0.911**	-0.826**	-0.980**	-0.685**	-0.846**	-0.858**	0.961**		0.992**	0.995**
	茎锰含量Stem manganese content	-0.870**	-0.960**	-0.897**	-0.861**	-0.961**	-0.744**	-0.895**	-0.882**	0.945**	0.987**		0.989**
	叶锰含量Leaf manganese content	-0.896**	-0.976**	-0.894**	-0.809**	-0.970**	-0.652**	-0.819**	-0.833**	0.950**	0.991**	0.976**