Response to fertilization of leaf functional traits of grassland plants with different mycorrhizal status

doi:10.11686/cyxb2022444

Abstract

Abstract:

In order to explore the effects of mycorrhizal status and fertilization on leaf traits of grassland plants， fertilization treatments were applied at 27 globally distributed grassland sites across four continents based on data made by Firn et al^［25］. The treatments included a control and full factorial combinations of nitrogen （N）， phosphorus （P）， and potassium （K）. After 3-4 years of nutrient addition， the leaf N， P and K contents and specific leaf areas （SLA） of dominant species in each plot were measured， and the mycorrhizal status of plants was determined based on published literature. On this basis， combined with the composition of dominant species， the grassland plants were divided into poaceous plants and non-poaceous species， and the responses of the two plant groups to difference in mycorrhizal status and fertilization treatments were further analyzed. It was found that mycorrhizal status was an important factor affecting the leaf traits of grassland plants. Especially for poaceous plants， the leaf nutrient content of non-obligate mycorrhizal （NOM） plants was significantly higher than that of obligate mycorrhizal （OM） plants. For non-poaceous grassland plants， the leaf traits of OM plants and NOM plants also showed some differences， but these were not significant overall. The effects of fertilization on the leaf traits of grassland plants were mainly to significantly increase the contents of N， P and K in leaves， but fertilization had little effect on SLA. Path analysis further showed that there was a significant positive correlation between fertilization and leaf nutrient content， while there was no significant correlation between fertilization and SLA. In addition， the leaf traits of OM and NOM plants in grassland were significantly affected by climatic factors and differed between treatment years. In conclusion， in poaceous plants， leaf traits were significantly affected by mycorrhizal status， while fertilization significantly increased the leaf nutrient content of grassland plants. The effects of climatic factors on the leaf traits of grassland plants and differences between years also merit further investigation.

Key words: mycorrhizal status, leaf traits, fertilization, grassland plants

Wen-jing WEI, Zhao-yong SHI, Meng-ge ZHANG, Shuang YANG, Wen-ya YANG. Response to fertilization of leaf functional traits of grassland plants with different mycorrhizal status[J]. Acta Prataculturae Sinica, 2023, 32(10): 104-114.

Figures/Tables 9

References 39

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施肥处理 Fertilization treatment	菌根状态 Mycorrhizal status		物种 Species		菌根状态与物种的交互作用Mycorrhizal status×species
施肥处理 Fertilization treatment	F	P	F	P	F	P
氮N	88.10	<0.001	53.63	<0.001	13.39	<0.001
磷P	82.14	<0.001	19.92	<0.001	9.79	<0.001
钾K	83.87	<0.001	33.02	<0.001	4.71	0.010
氮磷NP	58.18	<0.001	35.03	<0.001	8.88	<0.001
氮钾NK	78.60	<0.001	9.25	0.003	6.31	0.002
磷钾PK	64.85	<0.001	12.65	<0.001	4.72	0.009
氮磷钾NPK	89.23	<0.001	45.05	<0.001	13.61	<0.001

施肥处理 Fertilization treatment	菌根状态 Mycorrhizal status		物种 Species		菌根状态与物种的交互作用Mycorrhizal status×species
施肥处理 Fertilization treatment	F	P	F	P	F	P
氮N	88.10	<0.001	53.63	<0.001	13.39	<0.001
磷P	82.14	<0.001	19.92	<0.001	9.79	<0.001
钾K	83.87	<0.001	33.02	<0.001	4.71	0.010
氮磷NP	58.18	<0.001	35.03	<0.001	8.88	<0.001
氮钾NK	78.60	<0.001	9.25	0.003	6.31	0.002
磷钾PK	64.85	<0.001	12.65	<0.001	4.72	0.009
氮磷钾NPK	89.23	<0.001	45.05	<0.001	13.61	<0.001

施肥处理 Fertilization treatment	菌根状态 Mycorrhizal status		物种 Species		菌根状态与物种的交互作用Mycorrhizal status×species
施肥处理 Fertilization treatment	F	P	F	P	F	P
氮N	75.06	<0.001	45.50	<0.001	13.16	<0.001
磷P	71.33	<0.001	15.60	<0.001	8.80	<0.001
钾K	76.20	<0.001	27.23	<0.001	3.87	0.020
氮磷NP	45.71	<0.001	29.39	<0.001	8.44	<0.001
氮钾NK	67.77	<0.001	6.54	0.010	5.13	0.006
磷钾PK	53.27	<0.001	10.24	0.001	3.65	0.030
氮磷钾NPK	51.61	<0.001	8.63	0.004	2.74	0.070

施肥处理 Fertilization treatment	菌根状态 Mycorrhizal status		物种 Species		菌根状态与物种的交互作用Mycorrhizal status×species
施肥处理 Fertilization treatment	F	P	F	P	F	P
氮N	75.06	<0.001	45.50	<0.001	13.16	<0.001
磷P	71.33	<0.001	15.60	<0.001	8.80	<0.001
钾K	76.20	<0.001	27.23	<0.001	3.87	0.020
氮磷NP	45.71	<0.001	29.39	<0.001	8.44	<0.001
氮钾NK	67.77	<0.001	6.54	0.010	5.13	0.006
磷钾PK	53.27	<0.001	10.24	0.001	3.65	0.030
氮磷钾NPK	51.61	<0.001	8.63	0.004	2.74	0.070

施肥处理 Fertilization treatment	菌根状态 Mycorrhizal status		物种 Species		菌根状态与物种的交互作用Mycorrhizal status×species
施肥处理 Fertilization treatment	F	P	F	P	F	P
氮N	65.01	<0.001	40.10	<0.001	11.63	<0.001
磷P	64.69	<0.001	14.79	<0.001	7.03	0.001
钾K	73.02	<0.001	23.37	<0.001	2.54	0.080
氮磷NP	40.59	<0.001	6.91	<0.001	7.20	<0.001
氮钾NK	69.07	<0.001	6.40	0.010	4.82	0.009
磷钾PK	43.36	<0.001	8.96	0.003	1.92	0.150
氮磷钾NPK	43.97	<0.001	9.14	0.003	1.99	0.140