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草业学报 ›› 2013, Vol. 22 ›› Issue (5): 113-119.DOI: 10.11686/cyxb20130513

• 研究论文 • 上一篇    下一篇

花苜蓿内生放线菌多样性及群落结构

张波1,李小林1,2,江华明1,3,张小平1*   

  1. 1.四川农业大学资源环境学院,四川 成都 611130;
    2.四川省农业科学院土壤肥料研究所,四川 成都 610066;
    3.四川职业技术学院建筑与环境工程系,四川 遂宁 629000
  • 出版日期:2013-10-20 发布日期:2013-10-20
  • 通讯作者: E-mail:zhangxiaopingphd@126.com
  • 作者简介:张波(1987-),男,四川成都人,硕士。
  • 基金资助:
    国家863计划(2013AA102802-05)资助。

Diversity and community structures of the endophytic actinomycetes isolated from Medicago ruthenica

ZHANG Bo1, LI Xiao-lin1,2, JIANG Hua-ming1,3, ZHANG Xiao-ping1   

  1. 1.Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University, Chengdu 611130, China;
    2.Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;
    3.Department of Construction and Environmental Engineering, Sichuan Vocational and Technical College, Suining 629000, China
  • Online:2013-10-20 Published:2013-10-20

摘要: 为了了解药用植物花苜蓿内生放线菌的多样性和群落结构,将采自四川省甘孜地区的药用植物花苜蓿进行严格的表面消毒灭菌后,运用变性梯度凝胶电泳(denaturing gradient gel electrophoresis, DGGE)技术对其根茎叶花等样品的内生放线菌进行分析。结果表明,花苜蓿不同组织中内生放线菌多样性和群落结构存在明显差异。其内生放线菌的多样性指数(H)和丰富度(S)均是花>根>茎>叶,而均匀度(EH)则是叶>根>茎>花。不同组织样品之间的放线菌群落结构相似性差异明显,其中茎与叶的相似性最高,根与叶之间相似性最低,其他组织之间相似性均很低。DGGE条带回收测序结果显示,大部分克隆条带属于链霉菌属(Streptomyces),但仍有超过43%的条带属于稀有放线菌,且主条带A23和共有条带A12属于稀有放线菌,说明部分稀有放线菌在花苜蓿样品内是优势放线菌。该研究表明了花苜蓿根茎叶花组织的内生放线菌存在丰富的多样性,且不同组织内生放线菌多样性存在明显差异,其中花组织多样性最丰富。说明甘孜地区的药用植物花苜蓿可作为一种较理想的分离内生放线菌的植物资源。

Abstract: The denaturing gradient gel electrophoresis (DGGE) method was applied to investigate the diversity and community structures of the endophytic actinomycetes isolated from the different tissue parts of Medicago ruthenica grown in Ganzi, Sichuan Province. The result indicated that the diversity and community structures of the endophytic actinomycetes were significantly different among the plant tissue parts of root, stem, leaf and flower. The highest diversity index (H) and richness (S) were found in the plant flower, then, in the root, stem and leaf, respectively. The eveness (EH) of the actinomycetes was higher in the leaf, than that in the root, stem and flower, respectively. The similarity of the community structures was significantly different among the plant tissue parts, except that between the leaf and stem. The results of DGGE bands sequencing showed that the majority of the clones were the genus Streptomyces, the rest of 43% clones were rare actinomycetes. In addition, the dominant band A23 and common band A12 also showed the clone of rare actinomycetes, which indicated that some rare actinomycetes were the dominant species in the medicinal plant M. ruthenica. All of the results in this work revealed the high diversity of endophytic actinomycetes in the plant tissues of M. ruthenica, and the richest was found in the flower. M. ruthenica in Ganzi should be a good plant resource for isolation of endophytic actinomycetes.

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