老芒麦种质资源遗传多样性的SRAP分析

doi:10.11686/cyxb20140125

摘要/Abstract

摘要： 利用SRAP标记,对来自亚洲的84份老芒麦种质的遗传多样性和遗传关系进行了分析。23个引物组合共产生337条扩增带,其中203条为多态性带,多态性比率为60.24%。各种质间遗传相似系数的变幅为0.783~0.965,平均值为0.865。来自于青藏高原和蒙古的种质间的平均遗传相似性(GS)值最小(0.830),而来自于俄罗斯和蒙古的种质间的平均GS值最大(0.897)。对84份种质的聚类分析表明,供试种质可以划分成2大类,而且聚类结果与原始相似性矩阵间具有很高的吻合度(r=0.88)。同时,主向量分析(PCoA)也得到了与聚类分析类似的结果。方差分析(AMOVA)表明在总的遗传变异中有79.62%发生在地理类群内,有20.38%发生在类群间(Φ_ST=0.204),类群间和类群内的变异均为极显著(P<0.0001)。基于各地理类群间Φ_ST值进行的聚类分析也表明青藏高原类群明显区别于其他地理类群。这种聚类模式可能依赖于种质地理来源赋予其的特殊生态地理适应性。本研究结果对于今后老芒麦种质的利用和品种选育提供了有益信息。

Abstract: SRAP markers were used to analyze genetic diversity and genetic relationship among eighty-four Elymus sibiricus accessions from Asia. A set of 23 primer combinations yielded 337 bands,of which 203 were polymorphic (60.24%). Genetic similarity values (GS) among the accessions ranged between 0.783 and 0.965 with a mean of 0.865. On the average,Mongolian and Russian accessions were the most similar while,Mongolian and Qinghai-Tibet Plateau accessions were the most distant ones. Cluster analysis grouped the 84 accessions into two clusters,which has quite a high fit (r=0.88) to the original similarity matrix. Results of cluster analysis which was supported strongly by the principal coordinate analysis. The molecular variance analysis (AMOVA) showed that the proportion of variation explained by within geographic group and between geographic groups diversity was 0.7962 and 0.2038,respectively. Similar results were obtained when genetic diversity was estimated using the Shannon’s index of diversity. Based on pairwise Φ_ST between geographic groups,cluster analysis showed a clear demarcation between accessions from Qinghai-Tibet Plateau and the others as separate groups. The clustering pattern was probably dependent on geographic origin and ecological adaptability of the accessions. The results of present study can be useful in collecting germplasm and the establishment of core collections of E. sibiricus. The results in this study will provide useful information for the use of E. sibiricus germplasm and variety breeding.

中图分类号:

S543.03

顾晓燕，郭志慧，张新全，周永红，白史且，张昌兵，蒋忠荣，刘新，周朝杰，马啸. 老芒麦种质资源遗传多样性的SRAP分析[J]. 草业学报, 2014, 23(1): 205-216.

GU Xiao-yan,GUO Zhi-hui,ZHANG Xin-quan,ZHOU Yong-hong,BAI Shi-qie,ZHANG Chang-bing,JIANG Zong-rong,LIU Xin,ZHOU Chao-jie,MA Xiao. Genetic diversity of Elymus sibiricus germplasm resources revealed by SRAP markers[J]. Acta Prataculturae Sinica, 2014, 23(1): 205-216.

参考文献

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参考文献：
[1]Moreno-González J, Cubero J I. Selection strategies and choice of breeding methods[A]. In: Hayward M D, Bosemark N O, Romagosa I. Plant Breeding, Principles and Prospects[M]. London: Chapman & Hall, 1993: 281-313.
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[3]Bowden W M, Cody W J. Recognition of Elymus sibiricus L. from Alaska and the district of Mackenzie[J]. Bulletin of the Torrey Botanical Club, 1961, 88: 153-155. 
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[9]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theoretical and Applied Genetics, 2001, 103: 455-461.
[10]Williams J G K, Kubelik A R, Livak K J,et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Research, 1990, 18: 6531-6535.
[11]Vos P, Hogers R, Bleeker M,et al. AFLP: A new technique for DNA finger printing[J]. Nucleic Acids Research, 1995, 23: 4407-4414.
[12]Ferriol M, Pico B, Nuze F. Genetic diversity of a germplasm collection of Cucurbita pepousing SRAP and AFLP markers[J]. Theoretical and Applied Genetics, 2003, 107: 271-282.
[13]Budak H, Shearman R C, Parmaksiz I,et al. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers[J]. Theoretical and Applied Genetics, 2004, 108: 328-334.
[14]Vandemark G J, Ariss J J, Bauchan G A,et al. Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms[J]. Euphytica, 2006, 152: 9-16.
[15]李杰勤, 王丽华, 詹秋文, 等. 20个黑麦草品系的SRAP遗传多样性分析[J]. 草业学报, 2013, 22(2): 158-164.
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[17]Budak H, Shearman R C, Parmaksiz I,et al. Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs[J]. Theoretical and Applied Genetics, 2004, 109: 280-288.
[18]Doyle J J. DNA protocols for plants-CTAB total DNA isolation[A]. In: Hewitt G M, Johnston A. Molecular Techniques in Taxonomy[M]. Berlin, Germany： Springer-Verlag， 1991: 283-293.
[19]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Sciences, 1979, 76: 5269-5273.
[20]Mantel N. The detection of disease clustering and a generalized regression approach[J]. Cancer Research, 1967, 27: 209-220. 
[21]Yap I, Nelson R J. WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms[C]. Manila, Philippines: International Rice Research Institute (IRRI), 1995.
[22]Felsenstein J. Confidence limits on phylogenesis: an approach using the bootstrap[J]. Evolution, 1985, 39: 783-791.
[23]Rolf J F. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.1[M]. New York, USA：Exeter Software, Setaukel, 2000.
[24]Powell W, Morgante M, Andre C,et al. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) marker for germplasm analysis[J]. Molecular Breeding, 1996, 2: 225-238.
[25]Schneider S, Roessli D, Excoffier L. ARLEQUIN version 3.1: A Software for Population Genetics Data Analysis[M]. Switzerland：Genetics and Biometry Laboratory, University of Geneva, 2006.
[26]Excoffier L, Smouse P E, Quattro J M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction data[J]. Genetics, 1992, 131:479-491.
[27]Mizianty M. Variability and structure of natural populations of Elymus caninus (L.) L. based on morphology[J]. Plant Systematics and Evolution, 2005, 251: 199-216.
[28]Díaz O, Sun G L, Salomon B,et al. Levels and distribution of allozyme and RAPD variation in populations of Elymus fibrosis (Schrenk) Tzvel. (Poaceae)[J]. Genetic Resources and Crop Evolution, 2000, 47: 11-24. 
[29]马啸, 周永红, 于海清, 等. 野生垂穗披碱草种质的醇溶蛋白遗传多样性分析[J]. 遗传, 2006, 28(6): 699-706.
[30]Ruiz M, Aguiriano E. Analysis of duplication in the Spanish durum wheat collection maintained in the CRF-INIA on the basis of agro-morphological traits and gliadin proteins[J]. Genetic Resources and Crop Evolution, 2004, 51: 231-235.
[31]Zhang X Q, Salomom B, von Bothmer R. Application of random amplified polymorphic DNA markers to evaluate intraspecific genetic variation in the Elymus alaskanus complex (Poaceae)[J]. Genetic Resources and Crop Evolution, 2002, 49: 397-407.
[32]Sun G L, Díaz O, Salomon B,et al. Genetic diversity in Elymus caninus as revealed by isozyme, RAPD and microsatellite markers[J]. Genome, 1999, 42: 420-431.
[33]Agafonov A V, Baum B R, Bailey L G,et al. Differentiation in the Elymus dahuricuscomplex (Poaceae): evidence from grain proteins, DNA, and crossability[J]. Hereditas, 2002, 135: 277-289.
[34]Xu D H, Ban T. Phylogenetic and evolutionary relationships between Elymus humidusand other Elymus species based on sequencing of non-coding regions of cpDNA and AFLP of nuclear DNA[J]. Theoretical and Applied Genetics, 2004, 108: 1443-1448.
[35]Zeng B, Zhang X Q, Lan Y,et al. Evaluation of genetic diversity and relationships in orchardgrass（Dactylis glomerataL.) germplasm based on SRAP markers[J]. Canadian Journal of Plant Science, 2008, 88: 53-60. 
[36]鄢家俊，白史且，张新全, 等. 青藏高原老芒麦种质基于SRAP标记的遗传多样性研究[J]. 草业学报, 2010, 19(1): 173-183.
[37]Hamrick J L, Godt M J W. Conservation genetics of endemic plant species[A]. In: Avise J C, Hamrick J L. Conservation Genetics, Case Histories from Nature[M]. New York: Chapman and Hall, 1996: 281-304.
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[44]陈智华, 苗佳敏, 钟金城, 等. 野生垂穗披碱草种质遗传多样性的SRAP研究[J]. 草业学报, 2009, 18(5): 192-200.
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[46]Fu Y B, Peterson G W, Williams D,et al. Patterns of AFLP variation in a core subset of cultivated hexaploid oat germplasm[J]. Theoretical and Applied Genetics, 2005, 530: 530-539.
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