盐胁迫下豌豆幼苗对内外源NO的生理生化响应

doi:10.11686/cyxb20140522

摘要/Abstract

摘要：

以豌豆品种“陇豌一号”为材料,用NO供体硝普钠、NO清除剂c-PTIO及硝普钠类似物亚铁氰化钠处理豌豆幼苗,研究了内外源NO对盐胁迫下豌豆幼苗生长的影响。结果表明:添加外源NO能明显使盐胁迫下豌豆幼苗胚芽和胚根长、幼苗干重、根冠比及叶绿素含量增加(P<0.05),可溶性蛋白和可溶性糖含量升高,豌豆幼苗叶片SOD、POD和CAT 活性增加(P<0.05),叶片质膜相对透性降低,还能减缓MDA含量的上升并提高脯氨酸含量。内源NO对盐胁迫下豌豆幼苗胚芽生长、幼苗干重、可溶性糖含量、MDA含量以及SOD、CAT活性均具有明显的调节作用。因此,内外源NO均可缓解盐胁迫对豌豆幼苗的伤害。

Abstract:

A pea variety ‘longwan1’ was used as the experimental material to study the effect of endogenous and exogenous NO on the growth of pea seedlings under NaCl stress with NO donor sodium nitroprusside (SNP), NO scavenger c-PTIO and sodium nitroprusside analogues sodium ferrocyanide. It was found that adding exogenous NO increased the plumule and radicle length, seedling dry weight, root-shoot ratio, chlorophyll content, soluble protein and sugar levels, and the activities of SOD, POD and CAT of the pea seedlings. The content of proline was also increased under salt stress. Meanwhile, exogenous NO also alleviated the accumulation of MDA and decreased the membrane relative permeability in leaves of pea seedlings under salt stress. Endogenous NO had a significant effect on growth of germ, seedling dry weight, soluble sugar content, and MDA, SOD and CAT activity. In summary, exogenous and endogenous NO both alleviated the damage to pea seedlings caused by salt stress.

中图分类号:

S520.1

时振振,李胜,杨柯,马绍英,刘会杰,张品南,杨晓明. 盐胁迫下豌豆幼苗对内外源NO的生理生化响应[J]. 草业学报, 2014, 23(5): 193-200.

SHI Zhen-zhen,LI Sheng,YANG Ke,MA Shao-ying,LIU Hui-jie,ZHANG Pin-nan,YANG Xiao-ming. Physiological and biochemical response of pea seedlings to endogenous and exogenous NO under salt stress[J]. Acta Prataculturae Sinica, 2014, 23(5): 193-200.

参考文献

Reference:

[1] Zhu J K. Plant salt tolerance[J]. Trends in Plant Science, 2001, 6(2): 66-71.

[2] Zhao K F. Crop salt resistance crop physiology[M]. Beijing: Agricultural Press, 1990: 249-313.

[3] Ma X F, Zhang J Z, Song F B. Research advances in physiological ecology adaptation of plant salt-tolerance[J]. Science & Technology Review, 2011, 29(14): 76-79.

[4] Jing Y X, Yuan Q H. Effects of salt stress on seedling growth of alfalfa (Medicago sativa)and ion distribution in different alfalfa organs[J]. Acta Prataculturae Sinica, 2011, 20(2): 134-139.

[5] Zhang Y F, Liang Z W, Sui L, et al. Effect on physiological characteristic of Medicago sativa under saline-alkali stress at seeding stage[J]. Acta Prataculturae Sinica, 2009, 18(4): 230-235.

[6] Wang Z Q. China saline soil[M]. Beijing: Science Press, 1993.

[7] Toshio Yamaguchi, Eduardo Blumwald. Developing salt tolerant crop plants:challenges and opportunities[J]. Trends in Plant Science, 2005, 10(12): 616-620.

[8] Qin F M, Zhang H X, Wu Y, et al. Effects of salt stress on germination and seedling growth of Medicago falcata[J]. Acta Prataculturae Sinica, 2010, 19(4): 71-78.

[9] Liu A R, Zhang Y B, Zhong Z H, et al. Effects of salt stress on the growth and osmotica accumulation of Coleus blumei[J]. Acta Prataculturae Sinica, 2013, 22(2): 211-218.

[10] Liu J, Cai H, Liu Y, et al. A study on physiological characteristics and comparison of salt tolerance of two Medicago sativa at the seedling stage[J]. Acta Prataculturae Sinica, 2013, 22(2): 250-256.

[11] Zhang J L, Flowers T J, Wang S M. Mechanisms of sodium uptake by roots of higher plant[J]. Plant and Soil, 2010, 326(1): 45-60.

[12] Zhang J L, Shi H Z. Physiological and molecular mechanisms of plant salt tolerance[J]. Photosynthesis Research, 2013, 115(1): 1-22.

[13] Zou L N, Zhou Z Y, Yan S Y, et al. Effect of salt stress on physiological and biochemical characteristics of Amorpha fruticosa seedlings[J]. Acta Prataculturae Sinica, 2011, 20(3): 84-90.

[14] Zheng Z J, Wang S M, Zong C X. Chinese edible beans learn[M]. Beijing: China Agriculture Press, 1997.

[15] Zong C X, Wang Z G, Guan J P, et al. Pea seed Resource Description Specifications and Date Standard[M]. Beijing: China Agriculture Press, 2005.

[16] Qu X X, Huang Z Y. The adaptive strategies of halophyte seed germination[J]. Acta Ecologica Sinica, 2005, 25(9): 2389-2398.

[17] Lamattina L, Garca M C, Graziano M, et al. Nitricoxide: the versatility of an extensive signal molecule[J]. Annual Review of Plant Biology, 2003, 54: 109-136.

[18] Beligni M V, Lamattina L. Nitricoxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues[J]. Planta, 1999, 208: 337-344.

[19] Zhao M G, Tian Q Y, Zhang W H. Nitric oxide synthase dependent nitric oxide productions associated with salt tolerance in Arabidopsis[J]. Plant Physiology, 2007, 144(1): 206-217.

[20] Wang X Y, Shen W B, Xu L L. Exogenous nitric oxide alleviates osmotic stress-induced membrane lipid peroxidation in wheat seedling leaves[J]. Journal of Plant Physiology and Molecular Biology, 2004, 30(2): 195-200. 

[21] Akio U, Andre T J, Takashi H, et al. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J]. Plant Science, 2002, 163(3): 515-523. 

[22] Yong S Y. Effects of exogenous nitric oxide on seed germination and physiological characteristics of Pepper seedling under salt stress[D]. Lanzhou: Gansu Agricultural University, 2007.

[23] Wang F, Chang P P, Chen Y P, et al. Effect of exogenous nitric oxide on seedling growth and physiological characteristics of maize seedlings under cadmium stress[J]. Acta Prataculturae Sinica, 2013, 22(2): 178-186.

[24] Zhang Y Y, Liu J, Liu Y L. Nitric oxide alleviates growth inhibition of maize seedlings under NaCl stress[J]. Journal of Plant Physiology and Molecular Biology, 2004, 30(4): 455-459.

[25] Zhang S Z, Li P Y, Wang J H, et al. Effect of soaking seeds in exogenous NO donor SNP on catalpa speciosa seedling under salt stress[J]. Seed, 2013, 32(7): 22-24. 

[26] Zhao Y, Ai J, Wang Z X, et al. Effects of exogenous nitric oxide on chlorophyll fluorescence and antioxidant enzymes activity in amur grape (Vitis Amurensis Rupr) leaves under salt stress[J]. Journal of Nuclear Agricultural Sciences, 2013, 27(6): 867-872.

[27] Xie Y Z, He P, Wang C Y, et al. The effects of exgeneous CaCl2, SA and SNP on physiological traits of cassia obtusifolia L. seedlings under NaCl stress[J]. Journal of Southwest University(Natural Science), 2013, 35(3): 36-42.

[28] Liu J X, Hu H B, Wang X, et al. Effect of nitric oxide on proline accumulation in ryegrass seedlings subjected to salt stress[J]. Acta Agrectir Sinica, 2010, 18(6): 787-791.

[29] Li H S, Sun Q, Zhao S J, et al. Plant physiology and biochemistry experimental principles and techniques[M]. Beijing: Higher Education Press, 2000: 164-169, 260. 

[30] Niu J Y, Yang Q F. Crop cultivation methods[M]. Lanzhou: Gansu Ethnic Publishing House, 1998: 85-88.

[31] Zhang Z L, Qu W J. Plant physiology guide[M]. Beijing: Higher Education Press, 2003.

[32] Bohra J S, D rffling H, D rffling K. Salinity tolerance of rice with reference to endogenous and exogenous abscisic acid[J]. Journal of Agronomy and Crop Science, 1995, 174: 79-86.

[33] Wang F H, Chen S C, Li C H, et al. Effect of exogenous NO donor on seeds germination of cabbage under NaCl stress[J]. Seed, 2010, 29(2): 9-12.

[34] Jiang M Y, Yang W Y, Xu J, et al. Active oxygen damage effect of chlorophyll degradation in rice seedlings under osmotic stress[J]. Acta Botanica Sinica, 1994, 36(4): 289-295. 

[35] Li W Y, Xu W H, Hu X F, et al. Effects of Zinc stress on growth, physiological and biochemical and Zn uptake of Ryegrass (Lolium perenne L.)[J]. Transactions of the Chinese Society of Agricultural Engineering, 2007, 23(5): 190-194.

[36] Ruan H H, Shen W B, Ye M B, et al. Nitric oxide protective effect against oxidative damage under salt stress in wheat leaves[J]. Chinese Science Bulletin, 2001, 46(23): 1993-1997.

[37] Wu X X, Zhu Y L, Zhu W M, et al. Protective effects of exogenous nitric oxide on oxidative damage in tomato seedling leaves under NaCl stress[J]. Scientia Agricultura Sinica, 2006, 39(3): 575-581. 

[38] Zhang Y K, Cui X M, Yang S X, et al. Effects of exogenous nitric oxide on active oxygen metabolism and photosynthetic characteristics of tomato seedlings under cadmium stress[J]. Chinese Journal of Appled Ecology, 2010, 21(6): 1432-1438. 

[39] Zhu X J, Liang Y C, Yang J S, et al. Effect of exogenous calcium on antioxidant enzyme activity and lipid peroxidation of rice seedlings under salt stress[J]. Acta Pedologica Sinica, 2005, 42(3): 453-458.

[40] Jiao J, Wang X F, Yang F J, et al. Effects of exogenous NO on the growth and antioxidant enzyme activities of cucumber seedlings under NO_3~- stress[J]. Chinese Journal of Applied Ecology, 2009, 20(12): 3009-3012.

[41] Yu J H, Yong S Y, Zhang J B, et al. Protective effects of exogenous nitric oxide on oxidative damage in pepper seedlings under NaCl stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2007, 27(9): 1801-1806.

[42] Feng R Z, Wei Q.Alleviative effects of exogenous nitric oxide on oxidative damage metabolism in A vena staiva seedings under NaCl stress[J].Grassland and Turf, 2012, 32(6): 7-10.

[43] Wu X X, Chen J L, Cha D S, et al. Effects of exogenous nitric oxide on reactive oxygen metabolism in tomato seedlings under NaCl stress[J]. Plant Nutrition and Fertilizer Science , 2009, 15(2): 422-428.

[44] Xiao Q, Zheng H L. Nitric oxide and plant stress response[J]. Plant Physiology Communications, 2004, 40(3): 379-384. 

[45] Lu X, Shi W D, Wang Y L, et al. Effects of exogenous nitric oxide on activity of antioxidative enzyme and growth of oat seedlings under salt stress[J]. Pratacultural Science, 2011, 28(12): 2150-2156.

[46] Zhou W H, Shi S L, Kou J T. Effect of nitric oxide on alfalfa seed germination under Nacl stress[J]. Journal of Nuclear Agricultural Sciences , 2012, 26(4): 710-716.

[47] Fan H F, Guo S R, DuAN J J, et al. Effects of nitric oxide on the growth and glutathione dependent antioxidative system in cucumber(Cucum is sativus L.)[J]. Acta Ecologica Sinica, 2008, 28(6): 2511-2517.

参考文献：

[1] Zhu J K. Plant salt tolerance[J]. Trends in Plant Science, 2001, 6(2): 66-71.

[2] 赵可夫. 作物抗盐生理[M]. 北京: 农业出版社, 1990: 249-313.

[3] 马献发, 张继舟, 宋凤斌. 植物耐盐的生理生态适应性研究进展[J]. 科技导报, 2011, 29(14): 76-79.

[4] 景艳霞, 袁庆华. NaCl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J]. 草业学报, 2011, 20(2): 134-139.

[5] 张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响[J]. 草业学报, 2009, 18(4): 230-235.

[6] 王遵亲. 中国盐渍土[M]. 北京: 科学出版社, 1993.

[7] Toshio Yamaguchi, Eduardo Blumwald. Developing salt tolerant crop plants:challenges and opportunities[J]. Trends in Plant Science, 2005, 10(12): 616-620.

[8] 秦峰梅, 张红香, 武祎, 等. 盐胁迫对黄花苜蓿发芽及幼苗生长的影响[J]. 草业学报, 2010, 19(4): 71-78.

[9] 刘爱荣, 张远兵, 钟泽华, 等. 盐胁迫对彩叶草生长和渗透调节物质积累的影响[J]. 草业学报, 2013, 22(2): 211-218.

[10] 刘晶, 才华, 刘莹, 等. 两种紫花苜蓿苗期耐盐生理特性的初步研究及其耐盐性比较[J]. 草业学报, 2013, 22(2): 250-256.

[11] Zhang J L, Flowers T J, Wang S M. Mechanisms of sodium uptake by roots of higher plant[J]. Plant and Soil, 2010, 326(1): 45-60.

[12] Zhang J L, Shi H Z. Physiological and molecular mechanisms of plant salt tolerance[J]. Photosynthesis Research, 2013, 115(1): 1-22.

[13] 邹丽娜, 周志宇, 颜淑云, 等. 盐分胁迫对紫穗槐幼苗生理生化特性的影响[J]. 草业学报, 2011, 20(3): 84-90.

[14] 郑卓杰, 王述民, 宗绪晓. 中国食用豆类学[M]. 北京: 中国农业出版社, 1997.

[15] 宗绪晓, 王志刚, 关建平, 等. 豌豆种资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2005.

[16] 渠晓霞, 黄振英. 盐生植物种子萌发对环境的适应对策[J]. 生态学报, 2005, 25(9): 2389-2398.

[17] Lamattina L, Garca M C, Graziano M, et al. Nitricoxide: the versatility of an extensive signal molecule[J]. Annual Review of Plant Biology, 2003, 54: 109-136.

[18] Beligni M V, Lamattina L. Nitricoxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues[J]. Planta, 1999, 208: 337-344.

[19] Zhao M G, Tian Q Y, Zhang W H. Nitric oxide synthase dependent nitric oxide productions associated with salt tolerance in Arabidopsis[J]. Plant Physiology, 2007, 144(1): 206-217.

[20] 王宪叶, 沈文飚, 徐朗莱. 外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的缓解作用[J]. 植物生理与分子生物学学报, 2004, 30(2): 195-200. 

[21] Akio U, Andre T J, Takashi H, et al. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J]. Plant Science, 2002, 163(3): 515-523. 

[22] 雍山玉. 外源一氧化氮对盐胁迫下辣椒种子萌发和幼苗生理特性的影响[D]. 兰州: 甘肃农业大学, 2007.

[23] 王芳, 常盼盼, 陈永平, 等. 外源NO对镉胁迫下玉米幼苗生长和生理特性的影响[J]. 草业学报, 2013, 22(2): 178-186.

[24] 张艳艳, 刘俊, 刘友良. 一氧化氮缓解盐胁迫对玉米生长的抑制作用[J]. 植物生理与分子生物学学报, 2004, 30(4): 455-459.

[25] 张宋智, 李平英, 王军辉, 等. 外源 NO 供体 SNP 浸种对盐胁迫下黄金树幼苗生长的影响[J]. 种子, 2013, 32(7): 22-24. 

[26] 赵滢, 艾军, 王振兴, 等. 外源 NO 对 NaCl 胁迫下山葡萄叶片叶绿素荧光和抗氧化酶活性的影响[J]. 核农学报, 2013, 27(6): 867-872.

[27] 谢英赞, 何平, 王朝英, 等. 外源Ca2+, SA, NO对盐胁迫下决明幼苗生理特性的影响[J]. 西南大学学报, 2013, 35(3): 36-42.

[28] 刘建新, 胡浩斌, 王鑫, 等. 一氧化氮参与盐胁迫下黑麦草幼苗脯氨酸积累的调控[J]. 草地学报, 2010, 18(6): 787-791.

[29] 李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理和技术[M]. 北京：高等教育出版社, 2000: 164-169, 260. 

[30] 牛俊义, 杨祈峰. 作物栽培学研究方法[M]. 兰州：甘肃民族出版社, 1998: 85-88.

[31] 张志良, 瞿伟菁. 植物生理学指导[M]. 北京：高等教育出版社, 2003.

[32] Bohra J S, Drffling H, Drffling K. Salinity tolerance of rice with reference to endogenous and exogenous abscisic acid[J]. Journal of Agronomy and Crop Science, 1995, 174: 79-86.

[33] 王凤华, 陈双臣, 李春花, 等. 外源一氧化氮供体SNP对盐胁迫下甘蓝种子萌发的影响[J]. 种子, 2010, 29(2): 9-12.

[34] 蒋明义, 杨文英, 徐江, 等. 渗透胁迫下水稻幼苗中叶绿素降解的活性氧损伤作用[J]. 植物学报, 1994, 36(4): 289-295. 

[35] 李文一, 徐卫红, 胡小凤, 等. Zn胁迫对黑麦草幼苗生长、生理生化及Zn吸收的影响[J]. 农业工程学报, 2007, 23(5): 190-194.

[36] 阮海华,沈文飚, 叶茂炳,等. 一氧化氮对盐胁迫下小麦叶片的氧化损伤的保护效应[J]. 科学通报， 2001, 46(23): 1993-1997.

[37] 吴雪霞, 朱月林, 朱为民, 等. 外源一氧化氮对NaCl胁迫下番茄幼苗叶片氧化损伤的保护效应[J]. 中国农业科学, 2006, 39(3): 575-581. 

[38] 张义凯, 崔秀敏, 杨守祥, 等. 外源NO对镉胁迫下番茄活性氧代谢及光合特性的影响[J]. 应用生态学报, 2010, 21(6): 1432-1438. 

[39] 朱晓军, 梁永超, 杨劲松, 等. 钙对盐胁迫下水稻幼苗抗氧化酶活性和膜脂过氧化作用的影响[J]. 土壤学报, 2005, 42(3): 453-458.

[40] 焦娟, 王秀峰, 杨凤娟, 等. 外源一氧化氮对硝酸盐胁迫下黄瓜幼苗生长及抗氧化酶活性的影响[J]. 应用生态学报, 2009, 20(12): 3009-3012.

[41] 郁继华, 雍山玉, 张洁宝, 等. 外源NO对NaCl胁迫下辣椒幼苗氧化损伤的保护效应[J]. 西北植物学报, 2007, 27(9): 1801-1806.

[42] 冯瑞章, 魏琴. 外源NO对NaCl胁迫下燕麦幼苗氧化损伤的缓解效应[J]. 草原与草坪, 2012, 32(6): 7-10.

[43] 吴雪霞, 陈建林, 查丁石, 等. 外源一氧化氮对NaCl胁迫下番茄幼苗活性氧代谢的影响[J]. 植物营养与肥料学报, 2009, 15(2): 422-428.

[44] 肖强, 郑海雷. 一氧化氮与植物胁迫响应[J]. 植物生理学通讯, 2004, 40(3): 379-384. 

[45] 芦翔, 石卫东, 王宜伦, 等. 外源NO对NaCl胁迫下燕麦幼苗抗氧化酶活性和生长的影响[J]. 草业科学, 2011, 28(12): 2150-2156.

[46] 周万海, 师尚礼, 寇江涛. 一氧化氮对NaCl胁迫下苜蓿种子萌发的影响[J]. 核农学报, 2012, 26(4): 710-716.

[47] 樊怀福, 郭世荣, 段九菊, 等. 外源NO对NaCl胁迫下黄瓜幼苗生长和谷胱甘肽抗氧化酶系统的影响[J]. 生态学报, 2008, 28(6): 2511-2517.