Mn2+、Pb2+对野生垂穗披碱草种子萌发与幼苗生长的影响

doi:10.11686/cyxb2017387

草业学报 ›› 2018, Vol. 27 ›› Issue (3): 194-200.DOI: 10.11686/cyxb2017387

Mn²⁺、Pb²⁺对野生垂穗披碱草种子萌发与幼苗生长的影响

徐雅梅¹, 王传旗¹, 武俊喜², 张文静¹, 王小川¹, 赤列催珍¹, 徐德飞¹, 包赛很那¹, 苗彦军^{1, *}

1.西藏农牧学院,西藏林芝 860000;
2.中国科学院地理科学与资源研究所,北京 100101;

收稿日期:2017-09-14 修回日期:2017-10-27 出版日期:2018-03-20 发布日期:2018-03-20
通讯作者: * E-mail:myj666@126.com
作者简介:徐雅梅(1971-),女,内蒙古牙克石人,副教授,硕士。E-mail:670003231@qq.com
基金资助:
国家重点研发计划项目(2016YFC0502002),西藏自治区科技厅重点项目(2016ZDKJZC-15)和国家自然基金(31560142)资助

Effects of Mn²⁺ and Pb²⁺ on seed germination and seedling growth of Elymus nutans

XU Ya-mei¹, WANG Chuan-qi¹, WU Jun-xi², ZHANG Wen-jing¹, WANG Xiao-chuan¹, Chilie Cuizhen¹, XU De-fei¹, Baosai Heina¹, MIAO Yan-jun^1,*

1.Tibet Agriculture and Animal Husbandry College, Nyingchi 860000, China;
2.Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;

Received:2017-09-14 Revised:2017-10-27 Online:2018-03-20 Published:2018-03-20

摘要/Abstract

摘要： 研究了西藏巴青县野生垂穗披碱草种子萌发与幼苗生长对重金属Mn²⁺、Pb²⁺胁迫的生理响应机制,为西藏矿区周边污染土壤和植被修复提供依据。选取西藏巴青县野生垂穗披碱草为试验材料,采用纸上发芽法研究了重金属Mn²⁺、Pb²⁺对其种子萌发和幼苗生长的影响。结果表明,Mn²⁺、Pb²⁺离子胁迫对垂穗披碱草种子萌发和幼苗生长具有极显著的影响(P<0.01);随着Mn²⁺、Pb²⁺浓度的增大,垂穗披碱草种子发芽率、发芽势、发芽指数、活力指数及胚芽长表现为下降的趋势;在200 mg·L^-1 Mn²⁺、Pb²⁺溶液浓度培养下垂穗披碱草种子根较对照略长;随着Mn²⁺、Pb²⁺溶液浓度的升高,垂穗披碱草幼苗叶片叶绿素a和叶绿素b含量持续降低而电导率和MDA含量呈上升趋势。垂穗披碱草对Mn²⁺、Pb²⁺具有一定的耐受性,可适用于Mn²⁺、Pb²⁺轻度污染土壤。

Abstract: In order to provide reference for contaminated soil and vegetation restoration in the Tibetan mining area, a study has been undertaken of the physiological response of Elymus nutans’ seed germination and seedling growth to heavy metal Mn²⁺ and Pb²⁺ stress in Baqing county, Tibet. Select wild E. nutans from the county were used as test materials for investigation using germination methods. The results showed that Mn²⁺ and Pb²⁺ ion stress had a significant effect on seed germination and seedling growth. With increasing Mn²⁺ and Pb²⁺ concentrations, the germination percentage, germination vigor, germination index, vigor index and bud length of E. nutans showed a declining trend. Under a solution concentration of 200 mg·L^-1 Mn²⁺ and Pb²⁺, the shoot length of E. nutans was slightly longer than that of the control. With increasing Mn²⁺ and Pb²⁺ concentrations, the contents of chlorophyll a and chlorophyll b in leaves decreased while electrical conductivity and malondialdehyde content showed a rising trend. This study suggests that E. nutans has some tolerance to Mn²⁺ and Pb²⁺ ion stress that makes it a useful application for soils that are mildly contaminated with these toxins.

徐雅梅, 王传旗, 武俊喜, 张文静, 王小川, 赤列催珍, 徐德飞, 包赛很那, 苗彦军. Mn²⁺、Pb²⁺对野生垂穗披碱草种子萌发与幼苗生长的影响[J]. 草业学报, 2018, 27(3): 194-200.

XU Ya-mei, WANG Chuan-qi, WU Jun-xi, ZHANG Wen-jing, WANG Xiao-chuan, Chilie Cuizhen, XU De-fei, Baosai Heina, MIAO Yan-jun. Effects of Mn²⁺ and Pb²⁺ on seed germination and seedling growth of Elymus nutans[J]. Acta Prataculturae Sinica, 2018, 27(3): 194-200.

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Mn²⁺、Pb²⁺对野生垂穗披碱草种子萌发与幼苗生长的影响

Effects of Mn²⁺ and Pb²⁺ on seed germination and seedling growth of Elymus nutans

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