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草业学报 ›› 2018, Vol. 27 ›› Issue (3): 194-200.DOI: 10.11686/cyxb2017387

• 研究简报 • 上一篇    下一篇

Mn2+、Pb2+对野生垂穗披碱草种子萌发与幼苗生长的影响

徐雅梅1, 王传旗1, 武俊喜2, 张文静1, 王小川1, 赤列催珍1, 徐德飞1, 包赛很那1, 苗彦军1, *   

  1. 1.西藏农牧学院,西藏 林芝 860000;
    2.中国科学院地理科学与资源研究所,北京 100101;
  • 收稿日期:2017-09-14 修回日期:2017-10-27 出版日期:2018-03-20 发布日期:2018-03-20
  • 通讯作者: * E-mail:myj666@126.com
  • 作者简介:徐雅梅(1971-),女,内蒙古牙克石人,副教授,硕士。E-mail:670003231@qq.com
  • 基金资助:
    国家重点研发计划项目(2016YFC0502002),西藏自治区科技厅重点项目(2016ZDKJZC-15)和国家自然基金(31560142)资助

Effects of Mn2+ and Pb2+ on seed germination and seedling growth of Elymus nutans

XU Ya-mei1, WANG Chuan-qi1, WU Jun-xi2, ZHANG Wen-jing1, WANG Xiao-chuan1, Chilie Cuizhen1, XU De-fei1, Baosai Heina1, MIAO Yan-jun1,*   

  1. 1.Tibet Agriculture and Animal Husbandry College, Nyingchi 860000, China;
    2.Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
  • Received:2017-09-14 Revised:2017-10-27 Online:2018-03-20 Published:2018-03-20

摘要: 研究了西藏巴青县野生垂穗披碱草种子萌发与幼苗生长对重金属Mn2+、Pb2+胁迫的生理响应机制,为西藏矿区周边污染土壤和植被修复提供依据。选取西藏巴青县野生垂穗披碱草为试验材料,采用纸上发芽法研究了重金属Mn2+、Pb2+对其种子萌发和幼苗生长的影响。结果表明,Mn2+、Pb2+离子胁迫对垂穗披碱草种子萌发和幼苗生长具有极显著的影响(P<0.01);随着Mn2+、Pb2+浓度的增大,垂穗披碱草种子发芽率、发芽势、发芽指数、活力指数及胚芽长表现为下降的趋势;在200 mg·L-1 Mn2+、Pb2+溶液浓度培养下垂穗披碱草种子根较对照略长;随着Mn2+、Pb2+溶液浓度的升高,垂穗披碱草幼苗叶片叶绿素a和叶绿素b含量持续降低而电导率和MDA含量呈上升趋势。垂穗披碱草对Mn2+、Pb2+具有一定的耐受性,可适用于Mn2+、Pb2+轻度污染土壤。

Abstract: In order to provide reference for contaminated soil and vegetation restoration in the Tibetan mining area, a study has been undertaken of the physiological response of Elymus nutans’ seed germination and seedling growth to heavy metal Mn2+ and Pb2+ stress in Baqing county, Tibet. Select wild E. nutans from the county were used as test materials for investigation using germination methods. The results showed that Mn2+ and Pb2+ ion stress had a significant effect on seed germination and seedling growth. With increasing Mn2+ and Pb2+ concentrations, the germination percentage, germination vigor, germination index, vigor index and bud length of E. nutans showed a declining trend. Under a solution concentration of 200 mg·L-1 Mn2+ and Pb2+, the shoot length of E. nutans was slightly longer than that of the control. With increasing Mn2+ and Pb2+ concentrations, the contents of chlorophyll a and chlorophyll b in leaves decreased while electrical conductivity and malondialdehyde content showed a rising trend. This study suggests that E. nutans has some tolerance to Mn2+ and Pb2+ ion stress that makes it a useful application for soils that are mildly contaminated with these toxins.