围封条件下荒漠草原两种植物群落土壤理化性状与酶活性的研究

doi:10.11686/cyxb2018392

摘要/Abstract

摘要： 围栏封育是退耕还林还草工程措施之一,不仅有助于草场植被的恢复,也有助于土壤特性的改善。以宁夏盐池县荒漠草原沙芦草群落和苦豆子群落为研究对象,通过对土壤基本理化性状及4种常见土壤酶活性的测定,研究围封内外两种植物群落的土壤理化性状与酶活性的变化特征。结果表明: 1)较围栏外,围栏内的沙芦草群落0~10 cm土壤含水量、全氮、全磷均显著提高,土壤容重、pH值、碱解氮和速效磷含量显著降低,10~20 cm和20~40 cm土层的土壤理化性状之间无显著差异;苦豆子群落除土壤容重显著降低外,围封显著提高了土壤含水量、pH值、有机碳、全氮、全磷、碱解氮和速效磷含量。2)围栏内沙芦草群落中土壤脲酶活性显著降低,而土壤蔗糖酶活性在表层(0~10 cm)显著提高,过氧化氢酶和磷酸酶活性变化不显著;苦豆子群落围栏内土壤磷酸酶活性显著降低,蔗糖酶和脲酶的活性显著提高,过氧化氢酶活性变化不显著。3)两种植物群落中的土壤酶活性与土壤理化性状之间的相关性不同。苦豆子群落中土壤蔗糖酶活性、土壤脲酶活性与土壤养分之间均极显著正相关(P<0.01),而磷酸酶活性与之呈负相关关系;沙芦草群落中脲酶活性与土壤有机碳、全磷、速效磷含量均为极显著正相关关系(P<0.01),而过氧化氢酶活性与土壤有机碳、全氮、碱解氮含量之间呈显著正相关关系(P<0.05)。研究证实,围封尽管能够增加2种植物群落的表层土壤(0~10 cm)含水量和土壤养分,但对4种土壤酶活性的影响没有表现出明显的规律性。

关键词: 植物群落, 荒漠草原, 封育, 土壤酶活性, 沙芦草, 苦豆子

Abstract: Enclosure (fencing) is a fundamental part of China’s current ‘Grain for green’ program, and facilitates both the recovery of vegetation, and the improvement of soil properties. This paper, reports research into the recovery of soil physical and chemical properties and changes in activities of 4 soil enzymes after fencing in Agropyron mongolicum, and Sophora alopecuroides plant communities in the desert steppe of Ningxia province. Adopting the method of space as a substitute for time, the typical sites of two communities were selected and investigated in Yanchi County of Ningxia. The results showed that: 1) After fencing, soil water content, total N and total P increased significantly; while soil bulk density, pH, alkali hydrolysable N and available P contents were significantly reduced in the 0-10 cm soil layer of the A. mongolicum community studied. However, there were no significant differences in soil physical and chemical properties in the 10-40 cm soil depth. In the S. alopecuroides community soil bulk density decreased significantly, while soil water content, pH, organic carbon, total N, total P, available N and available P were significantly increased in the 5-10 cm soil depth. 2) After fencing, in the A. mongolicum community soil urease activity decreased significantly, while soil sucrase activity in the surface layer (0-10 cm) significantly increased, and the activity of catalase and phosphatase did not change significantly. In the S. alopecuroides community soil phosphatase activity decreased significantly, the activities of sucrase and urease were significantly increased, and the activity of catalase changed non-significantly. 3) There were different correlations in the two plant communities between soil enzyme activities and soil physical and chemical properties. In the S. alopecuroides community there was a significant positive correlation between soil sucrase activity, soil urease activity and soil nutrient content (P<0.01), while the activity of phosphatase showed a remarkably negative correlation. In A. mongolicum communities, there were significant positive correlations between urease activity and soil organic carbon, total P and available P content, and also, significant positive correlations between catalase activity and soil organic carbon, total N, and alkali hydrolysable N (P<0.05). In conclusion, although the water content and soil nutrient status of surface soil (0-10 cm) of the two communities were increased by fencing, no consistent changes in activities of the 4 soil enzymes studied were found.

Key words: plant communities, desert steppe, fencing, soil enzyme activity, Agropyron mongolicum, Sophora alopecuroides

李国旗, 赵盼盼, 邵文山, 靳长青. 围封条件下荒漠草原两种植物群落土壤理化性状与酶活性的研究[J]. 草业学报, 2019, 28(7): 49-59.

LI Guo-qi, ZHAO Pan-pan, SHAO Wen-shan, JIN Chang-qing. Studies on the soil physical and chemical properties and enzyme activities of two fenced plant communities in desert steppe grassland[J]. Acta Prataculturae Sinica, 2019, 28(7): 49-59.

参考文献

[1] Lü G F, Wu Y S, Li H, et al. Microorganisms, soil nutrient and enzyme activity changing with the proceeding of desert steppe degradation in Inner Mongolia. Journal of Desert Research, 2010, 30(1): 104-109.
吕桂芬, 吴永胜, 李浩, 等. 荒漠草原不同退化阶段土壤微生物、土壤养分及酶活性的研究. 中国沙漠, 2010, 30(1): 104-109.
[2] Liu R T, Yang X G, Song N P, et al. Soil properties following growing process of artificial forests (Caragana microphylla) in desert steppe. Journal of Soil and Water Conservation, 2012, 26(4): 108-112.
刘任涛, 杨新国, 宋乃平, 等.荒漠草原区固沙人工柠条林生长过程中土壤性质演变规律.水土保持学报, 2012, 26(4): 108-112.
[3] Shirato Y, Zhang T H, Ohkuro T, et al. Changes in topographical features and soil properties after exclosure combined with sand-fixing measures in Horqin sandy land, northern China. Soil Science & Plant Nutrition, 2005, 51(1): 61-68.
[4] Suo N J, Tan Y R, Zhu W X, et al. A study on soil enzyme activity in four different grasslands of the Easter Tibetan Plateau. Acta Prataculturae Sinica, 2012, 21(4): 10-15.
索南吉, 谈嫣蓉, 朱炜歆, 等. 青藏高原东缘不同草地类型土壤酶活性研究. 草业学报, 2012, 21(4): 10-15.
[5] Shu Y Y, Huang J S, Zhao G J, et al. Effects of afforestation with different tree species on soil enzyme activities and nutrient content in eastern Qinghai-Tibetan Plateau, China. Acta Ecologica Sinica, 2016, 36(2): 394-402.
舒媛媛, 黄俊胜, 赵高卷, 等. 青藏高原东缘不同树种人工林对土壤酶活性及养分的影响. 生态学报, 2016, 36(2): 394-402.
[6] Ye X F, Yang C, Li Z, et al. Effects of green manure in corporation on soil enzyme activities and fertility in tobacco-planting soil. Plant Nutrition and Fertilizer Science, 2013, 19(2): 445-454.
叶协锋, 杨超, 李正, 等. 绿肥对植烟土壤酶活性及土壤肥力的影响. 植物营养与肥料学报, 2013, 19(2): 445-454.
[7] He D J, You H M, Xiao S H, et al. Soil enzyme activities under fallen logs and their environmental gradient analysis in the Tsuga longibracteata forest, Tianbaoyan national nature reserve. Acta Ecologica Sinica, 2017, 37(1): 118-126.
何东进, 游惠明, 肖石红, 等. 天宝岩长苞铁杉林倒木接触处土壤酶活性变化及其环境效应. 生态学报, 2017, 37(1): 118-126.
[8] Liu S, Xia X, Chen G, et al. Study progress on functions and affecting factors of soil enzymes. Chinese Agricultural Science Bulletin, 2011, 27(21): 1-7.
[9] Boerner R E J, Brinkman J A, Smith A. Seasonal variations in enzyme activity and organic carbon in soil of a burned and unburned hardwood forest. Soil Biology & Biochemistry, 2005, 37(8): 1419-1426.
[10] Yang N, Zou D S, Yang M Y, et al. Soil enzyme activities in different re-vegetation stages on sloping-land with purple soils in Hengyang of Hunan Province, China. Journal of Plant Nutrition and Fertilizer, 2013, (6): 1516-1524.
杨宁, 邹冬生, 杨满元, 等. 衡阳紫色土丘陵坡地不同植被恢复阶段土壤酶活性特征研究. 植物营养与肥料学报, 2013, (6): 1516-1524.
[11] Banerjee S, Bora S, Thrall P H, et al. Soil C and N as causal factors of spatial variation in extracellular enzyme activity across grassland-woodland ecotones. Applied Soil Ecology, 2016, 105: 1-8.
[12] Tang Y S, Ci E, Yan T M, et al. Relationship between soil enzyme activity and soil fertility of paddy fields under wheat-rice cropping system in a long-term experiment in Taihu lake region. Acta Pedologica Sinica, 2008, 45(5): 1000-1006.
唐玉姝, 慈恩, 颜廷梅, 等. 太湖地区长期定位试验稻麦两季土壤酶活性与土壤肥力关系. 土壤学报, 2008, 45(5): 1000-1006.
[13] Tan Y R, Du G Z, Chen D D, et al. Impact of grazing on the activities of soil enzymes and soil nutrient factors in an alpine meadow on the Qinghai-Tibetan plateau. Journal of Lanzhou University (Natural Sciences Edition), 2012, 48(1): 86-91.
谈嫣蓉, 杜国祯, 陈懂懂, 等. 放牧对青藏高原东缘高寒草甸土壤酶活性及土壤养分的影响. 兰州大学学报(自然科学版), 2012, 48(1): 86-91.
[14] Gao F, Wang B, Shi Y X, et al. The response of alpine grasslands ecosystem in the north Tibet to short-term enclosure. Acta Ecologica Sinica, 2017, 37(13): 1-9.
高凤, 王斌, 石玉祥, 等. 藏北古露高寒草地生态系统对短期围封的响应. 生态学报, 2017, 37(13): 1-9.
[15] Hewins D B, Fatemi F, Adams B, et al. Grazing, regional climate and soil biophysical impacts on microbial enzyme activity in grassland soil of western Canada. Pedobiologia, 2015, 58(5/6): 201-209.
[16] Shao W S, Li G Q, Chen K Y, et al. Comparative studies on soil enzymic activities of four kinds of common plant communities in desert steppe. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(3): 579-587.
邵文山, 李国旗, 陈科元, 等. 荒漠草原4种常见植物群落土壤酶活性比较. 西北植物学报, 2016, 36(3): 579-587.
[17] Li G Q, Shao W S, Zhao P P, et al. Effects of enclosure on the soil seed bank of two plant communities on the desert steppe. Acta Prataculturae Sinica, 2018, 27(6): 52-61.
李国旗, 邵文山, 赵盼盼, 等. 封育对荒漠草原两种植物群落土壤种子库的影响. 草业学报, 2018, 27(6): 52-61.
[18] Bao S D.Soil agro-chemistrical analysis (Third Edition). Beijing: China Agricultural Press, 2000: 30-81.
鲍士旦. 土壤农化分析(第3版). 北京: 中国农业出版社, 2000: 30-81.
[19] Guan S Y.Soil enzyme and its research method. Beijing: Agricultural Publishing House, 1986: 260-346.
关松荫. 土壤酶及其研究法. 北京: 农业出版社, 1986: 260-346.
[20] Yan Y C, Tang H P, Xing X P, et al. Advances in research on the effects of exclosure on grasslands. Acta Ecologica Sinica, 2009, 29(9): 5039-5046.
闫玉春, 唐海萍, 辛晓平, 等. 围封对草地的影响研究进展. 生态学报, 2009, 29(9): 5039-5046.
[21] Mekuria W, Veldkamp E, Haile M, et al. Effectiveness of exclosure to restore degraded soils as a result of overgrazing in Tigray, Ethiopia. Journal of Arid Environments, 2007, 69(2): 270-284.
[22] Li J B, Cao Q X, Tursunay R, et al. Effects of enclosure on soil physical and chemical quality and enzymic activity in grassland of Yili Valley in spring-autumn. Chinese Journal of Grassland, 2014, 36(1): 84-89.
李军保, 曹庆喜, 吐尔逊娜依·热依木江, 等. 围封对伊犁河谷春秋草场土壤理化性质及酶活性的影响. 中国草地学报, 2014, 36(1): 84-89.
[23] Huang R, Wang H, Wang H, et al. Effects of enclosure year on soil physicochemical properties of sandy grassland. Journal of Soil and Water Conservation, 2014, 28(1): 183-188.
黄蓉, 王辉, 王蕙, 等. 围封年限对沙质草地土壤理化性质的影响. 水土保持学报, 2014, 28(1): 183-188.
[24] Yan Y C, Tang H P, Chang R Y, et al. Study on the difference of vegetation and soil in typical steppe communities under different fenced time. Journal of Arid Land Resources & Environment, 2008, 22(2): 146-151.
[25] Li Y, Yan Z Y, Guo D, et al. Effects of fencing and grazing on vegetation and soil physical and chemical properties in an alpine meadow in the Qinghai Lake Basin. Acta Prataculturae Sinica, 2015, 24(10): 33-39.
李洋, 严振英, 郭丁, 等. 围封对青海湖流域高寒草甸植被特征和土壤理化性质的影响. 草业学报, 2015, 24(10): 33-39.
[26] Pei S, Fu H, Wan C.Changes in soil properties and vegetation following exclosure and grazing in degraded Alxa desert steppe of Inner Mongolia, China. Agriculture Ecosystems & Environment, 2008, 124(1): 33-39.
[27] Li X B, Chen L, Fan R X, et al. Effects of four typical plant community litter in put on soil physical and chemical properties under the fenced condition in desert steppe. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(1): 101-110.
李学斌, 陈林, 樊瑞霞, 等. 围封条件下荒漠草原4种典型植物群落枯落物输入对土壤理化性质的影响. 浙江大学学报(农业与生命科学版), 2015, 41(1): 101-110.
[28] Song Y.The allelopathic effects of Sophora alopecuroides L. and its influence on community diversity. Beijing: Beijing Forestry University, 2012.
宋洋. 苦豆子的化感作用及其对群落多样性的影响. 北京: 北京林业大学, 2012.
[29] Jia X H, Li Y S, Wu B, et al. Effects of plant restoration on soil microbial biomass in an arid desert in northern China. Journal of Arid Environments, 2017, 144: 192-200.
[30] Shan G L, Chu X H, Tian Q S, et al. Research on the dynamic changes of soil properties of typical steppe in the restoring process. Acta Prataculturae Sinica, 2012, 21(4): 1-9.
单贵莲, 初晓辉, 田青松, 等. 典型草原恢复演替过程中土壤性状动态变化研究. 草业学报, 2012, 21(4): 1-9.
[31] Bai Y F, Xu Z X, Li D X.On the small scale spatial heterogeneity of soil moisture, carbon and nitrogen in Stipa communities of the Inner Mongolia Plateau. Acta Ecologica Sinica, 2002, 22(8): 1215-1223.
白永飞, 许志信, 李德新. 内蒙古高原针茅草原群落土壤水分和碳、氮分布的小尺度空间异质性. 生态学报, 2002, 22(8): 1215-1223.
[32] Ding X H, Gong L, Wang D B, et al. Grazing effects on eco-stoichiometry of plant and soil in Hulunbeir, Inner Mogolia. Acta Ecologica Sinica, 2012, 32(15): 4722-4730.
丁小慧, 宫立, 王东波, 等. 放牧对呼伦贝尔草地植物和土壤生态化学计量学特征的影响. 生态学报, 2012, 32(15): 4722-4730.
[33] Yang H Y, Liu C Z, Liu Y M, et al. Impact of human trampling on biological soil crusts determined by soil microbial biomass, enzyme activities and nematode communities in a desert ecosystem. European Journal of Soil Biology, 2018, 87: 61-71.
[34] Wang X T, Nielsen U N, Yang X L, et al. Grazing induces direct and indirect shrub effects on soil nematode communities. Soil Biology and Biochemistry, 2018, 121: 193-201.
[35] Lu P, Guo J X, Zhu L.Soil catalase activity of main plant communities in Leymus chinensis grassland in northeast China. Chinese Journal of Applied Ecology, 2002, 13(6): 675-679.
鲁萍, 郭继勋, 朱丽. 东北羊草草原主要植物群落土壤过氧化氢酶活性的研究. 应用生态学报, 2002, 13(6): 675-679.
[36] Wen D R L, Zhang J N, Li G, et al. Effect of grazing disturbance on soil microorganisms and soil enzyme activities of Stipa baicalensis Rosev. Steppe. Acta Agrestia Sinica, 2010, 18(4): 517-522.
文都日乐, 张静妮, 李刚, 等. 放牧干扰对贝加尔针茅草原土壤微生物与土壤酶活性的影响. 草地学报, 2010, 18(4): 517-522.
[37] Shu S Y, Wang K L, Zhang W, et al. Soil alkaline phosphatase activity at different vegetation succession stages in karst peak-cluster depression. Chinese Journal of Ecology, 2010, 29(9): 1722-1728.
舒世燕, 王克林, 张伟, 等. 喀斯特峰丛洼地植被不同演替阶段土壤磷酸酶活性. 生态学杂志, 2010, 29(9): 1722-1728.
[38] Nausch M, Nausch G.Stimulation of peptidase activity in nutrient gradients in the Baltic Sea. Soil Biology & Biochemistry, 2000, 32(13): 1973-1983.
[39] Qiu L P, Zhang X C, Cheng J M.Influence of slope direction, position and fallow measurement of soil enzymatic activities in grassland at Yunwu Mountain. Acta Prataculturae Sinica, 2007, 16(1): 87-93.
邱莉萍, 张兴昌, 程积民. 坡向坡位和撂荒地对云雾山草地土壤酶活性的影响. 草业学报, 2007, 16(1): 87-93.
[40] Luo D, Wang M J, Zheng S L, et al. Effects of enclosure on soil microbial quantity and enzyme activity in desert steppe. Ecology and Environmental Sciences, 2016, 25(5): 760-767.
罗冬, 王明玖, 郑少龙, 等. 围封对荒漠草原土壤微生物数量及其酶活性的影响. 生态环境学报, 2016, 25(5): 760-767.
[41] Yang Y D, Feng X M, Hu Y G, et al. Effects of legume-oat intercropping on abundance and community structure of soil N₂-fixing bacteria. Chinese Journal of Applied Ecology, 2017, 28(3): 957-965.
杨亚东, 冯晓敏, 胡跃高, 等. 豆科作物间作燕麦对土壤固氮微生物丰度和群落结构的影响. 应用生态学报, 2017, 28(3): 957-965.
[42] Li L X.Introduction test of four perennial grass species. Acta Agrestia Sinica, 2018, 35(1): 264-266.
李莲香. 四种多年生禾本科牧草引种试验. 草地学报, 2018, 35(1): 264-266.
[43] Yang W Q, Zhong Z C, Tao J P, et al. Study on relationship between soil enzymic activities and plant species diversity in forest ecosystem of Jinyun Mountain. Scientia Silvae Sinicae, 2001, 37(4): 124-128.
杨万勤, 钟章成, 陶建平, 等. 缙云山森林土壤酶活性与植物多样性的关系. 林业科学, 2001, 37(4): 124-128.
[44] Zhang Q Z, Wu W L, Wang M X, et al. The effects of crop residue amendment and N rate on soil respiration. Acta Ecologica Sinica, 2005, 25(11): 2883-2887.
张庆忠, 吴文良, 王明新, 等. 秸秆还田和施氮对农田土壤呼吸的影响. 生态学报, 2005, 25(11): 2883-2887.
[45] Zhao S, Zhang J N, Lai X, et al. Effects of grazing and fencing on soil enzyme activity and soil physicochemical properties in Stipa Steppe in Hulunbeier, Inner Mongolia. Chinese Journal of Grassland, 2011, 33(1): 71-76.
赵帅, 张静妮, 赖欣, 等. 放牧与围封对呼伦贝尔针茅草原土壤酶活性及理化性质的影响. 中国草地学报, 2011, 33(1): 71-76.
[46] Si G C, Yuan Y L, Wang J, et al. Effects of fencing on microbial communities and soil enzyme activities in Damxung alpine grassland. Pratacultural Science, 2015, 32(1): 1-10.
斯贵才, 袁艳丽, 王建, 等. 围封对当雄县高寒草原土壤微生物和酶活性的影响. 草业科学, 2015, 32(1): 1-10.