锰胁迫对黄花草种子萌发及幼苗生理生化特征的影响

doi:10.11686/cyxb2019041

摘要/Abstract

摘要： 为探明锰胁迫下黄花草的抗性生理适应机制,以该植物种子为试验材料,研究了不同浓度锰(0,1000,5000,10000,15000和20000 μmol·L^-1)胁迫对黄花草种子萌发、幼苗生长的影响及胁迫7、15、30 d生理生化特征的变化。结果表明:1)随着锰浓度的增加,黄花草种子的发芽势、发芽率、发芽指数和活力指数均呈下降趋势;幼苗芽长、根长、生物量和根冠比则先增加后下降,呈现出“低促高抑”现象;2)1000~5000 μmol·L^-1锰处理增加了黄花草幼苗叶片叶绿素a、叶绿素b、叶绿素a+b含量和叶绿素a/b值;当浓度继续增加且胁迫时间延长时,叶绿素含量和叶绿素a/b值降低;3)在胁迫第7天,黄花草叶片超氧化物歧化酶(SOD)活性随锰浓度增加逐渐下降,过氧化物酶(POD)和过氧化氢酶(CAT)活性随锰浓度增加呈上升趋势;在胁迫第30天,SOD和POD活性均显著下降,CAT活性无明显下降;4)黄花草幼苗叶片中脯氨酸、可溶性蛋白和可溶性糖含量在胁迫第7、15天随锰浓度增加而增加,在胁迫30 d后则均下降;5)丙二醛(MDA)含量随着锰浓度的增加和胁迫时间的延长而显著增加。研究结果表明,1000~5000 μmol·L^-1锰处理会促进黄花草幼苗的生长;该植物可以通过不同抗氧化酶差异化响应及渗透调节物质含量的提高,减轻高浓度锰胁迫所造成的损伤,提高对锰的耐性。

关键词: 锰胁迫, 黄花草, 种子萌发, 抗氧化系统, 渗透调节

Abstract: In order to investigate the resistance and physiological adaption mechanisms of Cleome viscosa to manganese (Mn) stress, the effects of different concentrations of Mn²⁺ (0, 1000, 5000, 10000, 15000 and 20000 μmol·L^-1) on seed germination, seedling growth and the changes in physiological and biochemical characteristics of C. viscosa after 7, 15 and 30 days of stress, were assessed. The results showed that: 1) With the increasing Mn²⁺ concentration, the germination potential, germination rate, germination index and vigor index of C. viscosa declined, although the seedling growth was increased at the 1000 and 5000 μmol·L^-1 Mn²⁺ concentrations, displaying a phenomenon of “low to promote and high inhibition”. 2) Compared with the control, chlorophyll a, chlorophyll b, chlorophyll a+b content and the ratio of chlorophyll a∶b increased at the Mn²⁺ concentrations of 1000 and 5000 μmol·L^-1, while the chlorophyll content and the ratio of chlorophyll a∶b decreased with rising of Mn²⁺ concentration above 5000 μmol·L^-1 and with exposure time. 3) Under Mn²⁺ stress at day 7, superoxide dismutase (SOD) activity decreased with rising Mn²⁺ concentration, while the activities of peroxidase (POD) and catalase (CAT) increased; Under Mn²⁺ stress at day 30, the activities of SOD and POD decreased, while the activity of CAT didn’t decline. 4) Proline, soluble protein and soluble sugar of seedlings increased significantly with rising Mn²⁺ concentration at day 7 and day 15 of exposure, but had decreased after 30 days of stress. 5) Malondialdehyde content increased significantly with increasing of Mn²⁺ concentration and exposure time. In summary, the results showed that exposure to Mn²⁺ concentrations of 1000 and 5000 μmol·L^-1 promoted seedling growth of C. viscosa, and at higher Mn²⁺ concentrations this plant species can adjust its antioxidant system and enhance its osmotic adjustment to alleviate the damage caused by high Mn stress, thus imparting C. viscosa with a measure of Mn tolerance.

Key words: manganese stress, Cleome viscosa, seed germination, antioxidant system, osmotic adjustment

肖泽华, 李欣航, 潘高, 吴耀文, 杨灿鑫, 匡雪韶, 刘文胜. 锰胁迫对黄花草种子萌发及幼苗生理生化特征的影响[J]. 草业学报, 2019, 28(12): 75-84.

XIAO Ze-hua, LI Xin-hang, PAN Gao, WU Yao-wen, YANG Can-xin, KUANG Xue-shao, LIU Wen-sheng. Effects of manganese stress on seed germination, and seedling physiological and biochemical characteristics of Cleome viscosa[J]. Acta Prataculturae Sinica, 2019, 28(12): 75-84.

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