假苍耳不同部位水浸提液对五种十字花科植物化感作用的研究

doi:10.11686/cyxb2017398

摘要/Abstract

摘要： 探讨假苍耳根、茎浸提液对萝卜、白菜、油菜、芥菜、甘蓝5种十字花科植物化感效应和作用规律。本试验采用室内生物学测定法,研究不同浓度(10、20、30、40 g·L^-1)假苍耳不同部位浸提液对5种十字花科植物种子的萌发及幼苗生长的影响,阐明假苍耳浸提液对5种十字花科植物的化感作用及作用机制。结果表明:假苍耳根、茎浸提液对5种十字花科植物发芽率、发芽势、发芽指数、活力指数、根长、苗高、苗鲜重具有显著的化感作用(P<0.05),且5种植物之间存在一定的差异,对萝卜、白菜、油菜总体趋势表现为“低促高抑”的双重效应,而对芥菜、甘蓝表现为抑制作用;不同部位浸提液对5种十字花科植物抑制作用强弱茎浸提液>根浸提液,说明假苍耳茎浸提液中含有抑制5种植物种子萌发及幼苗生长的主要化感物质;随着浸提液浓度升高,假苍耳对十字花科植物化感作用增强。综合化感指数评价结果显示,假苍耳植株浸提液对供试植物种子萌发和幼苗生长均有显著影响。

关键词: 假苍耳, 浸提液, 化感作用, 种子萌发, 幼苗生长

Abstract: This study investigated the allelopathic effect of extracts of Iva xanthiifolia root and stem tissues on Raphanus sativus, Brassica campestris, Brassica pekinensis, Brassica juncea, and Brassica oleracea. In this study, the effects of different concentrations (10, 20, 30, 40 g·L^-1) aqueous extract on the germination and seedling growth of the above 5 Brassicaceae species were studied indoors to elucidate allelopathic effects of I. xanthiifolia on these crops indoors. Significant (P<0.05) allelopathic effects on the germination rate, germination potential, germination index, vigor index, root length, seedling height and fresh weight were found, and some differences in response among the five tested brassica species. A notable feature of the results were that growth promoted effects on seed germination and growth of R. sativus, B. pekinensis and B. campestris at the lowest treatment rate of 10 g·L^-1 I. xanthiifolia extract and inhibitory effects at higher rates, while B. juncea and B. oleracea were inhibited even at the lowest treatment rates. Stem extract of I. xanthiifolia was more potent than root extract, which indicated that the aqueous extract of the stem contained the main allelochemicals with inhibitory effects on seed germination and seedling growth. The results of this comprehensive allelopathy index evaluation, demonstrated that aqueous extracts of I. xanthiifolia exert a strong inhibitory effect on seedling growth of the five Brassicaceae species tested.

Key words: Iva xanthiifolia, aqueous extract, allelopathy, seed germination, seedling growth

姚树宽, 李凤兰, 彭丽娜, 冯旭, 董佳敏, 冯哲, 张俊峰, 赵巧芩, 冯珊珊, 徐永清, 胡宝忠. 假苍耳不同部位水浸提液对五种十字花科植物化感作用的研究[J]. 草业学报, 2018, 27(9): 56-66.

YAO Shu-kuan, LI Feng-lan, PENG Li-na, FENG Xu, DONG Jia-min, FENG Zhe, ZHANG Jun-feng, ZHAO Qiao-qin, FENG Shan-shan, XU Yong-qing, HU Bao-zhong. A study of the allelopathic effect of extracts from different parts of Iva xanthiifolia on five Brassicaceae species[J]. Acta Prataculturae Sinica, 2018, 27(9): 56-66.

参考文献

[1] Yue J H.Study on allelopathic effect of bamboo water extracts to Chinese fir seed germination and seedling. Fuzhou: Fujian Agriculture and Forestry University, 2010.
岳建华. 毛竹水浸提液对杉木种子发芽和幼苗化感作用研究. 福州: 福建农林大学, 2010.
[2] Rice E L.Allelopathy. Ecology, 1984, (1): 292-293.
[3] Yan F, Yang Z M.Review on research methods for alelopathy and allelochemicals in plants. Acta Ecologica Sinica, 2000, 20(4): 692-696.
阎飞, 杨振明. 植物化感作用(Allelopathy)及其作用物的研究方法. 生态学报, 2000, 20(4): 692-696.
[4] Inderjit, Callaway R M. Experimental designs for the study of allelopathy. Plant & Soil, 2003, 256(1): 1-11.
[5] Das R, Geethangili M, Majhi A, et al. A new highly oxygenated pseudoguaianolide from a collection of the flowers of Parthenium hysterophorus. Chemical & Pharmaceutical Bulletin, 2005, 53(7): 861-862.
[6] Wan H H.Allelopathic effect and decompositon dynamics of leaf litter of an invasive plant: Ageratina adenophora Sprengel (Compositae). Beijing: Chinese Academy of Agricultural Sciences, 2010.
万欢欢. 入侵植物紫茎泽兰叶片凋落物的化感作用及其降解动态. 北京: 中国农业科学院, 2010.
[7] Yang Y B, Lian J W, Chen H Y, et al. Allelopathic effects of invasive weeds Mikania micrantha on 2 species of forage grass in Southern China area. Bulletin of Biology, 2015, 50(5): 52-56.
杨依贝, 连嘉雯, 陈泓钰, 等. 华南地区2种优质牧草对入侵杂草薇甘菊的化感作用. 生物学通报, 2015, 50(5): 52-56.
[8] Wang R, Chen X, Ding G H.Comparison of the allelopathic effects between native and invasive Chromolaena odorata. Northern Horticulture, 2013, (10): 66-69.
王锐, 陈曦, 丁国华. 原产地和入侵地飞机草化感作用比较. 北方园艺, 2013, (10): 66-69.
[9] Lu Y M, Li H F.The allelopathy of Trifolium repens aqueous extract on ryegrass seeds. Jiangsu Agricultural Sciences, 2012, 40(5): 367-369.
卢艳敏, 李会芬. 白三叶草水浸液对黑麦草种子的化感作用. 江苏农业科学, 2012, 40(5): 367-369.
[10] Wang X F, Liu C S, Gao P, et al. Allelopathy of Panax notoginseng aqueous extracts on seed germination and seedling growth of several crops seeds. Chinese Agricultural Science Bulletin, 2014, (4): 299-303.
王雄飞, 刘春生, 高鹏, 等. 三七水提液对几种植物种子萌发和幼苗生长的化感作用. 中国农学通报, 2014, (4): 299-303.
[11] Dong Q F, Cheng Z H.Study on allelopathy of top part aqueous extracts of lily plant. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(2): 144-147.
董沁方, 程智慧. 百合地上部分水浸液的化感效应研究. 西北农业学报, 2006, 15(2): 144-147.
[12] Liu X D, Chen L, Yang X G, et al. Allelopathic effects of aqueous extracts from Caragana intermedia’s flower on seed germination and seeding growth of three kinds of crop. Northern Horticulture, 2016, (12): 65-70.
刘学东, 陈林, 杨新国, 等. 中间锦鸡儿花水浸提液对三种作物种子萌发及幼苗生长的化感作用. 北方园艺, 2016, (12): 65-70.
[13] Guan G Q.A new invasive weed in our country-Iva xanthiifolia. Plant Quarantine, 1983, (5): 44-49.
关广清. 一种新侵入我国的杂草——假苍耳. 植物检疫, 1983, (5): 44-49.
[14] Milanova S D.Iva xanthiifolia Nutt.-a new species in the adventive flora in Bulgaria. llth European Weed Research Society Symposium, Basiel, Switzerland, 1999: 40.
[15] Zhao W, Tao B.Chemical control of Iva xanthifolia. Journal of Northeast Agricultural University, 2010, 41(6): 32-36.
赵微, 陶波. 假苍耳的化学防除. 东北农业大学学报, 2010, 41(6): 32-36.
[16] Jia J.The invasive characteristics of the forestry harmful plant Iva xanthifolia Nutt. Harbin: Northeast Forestry University, 2007.
贾晶. 林业有害植物假苍耳的入侵特性研究. 哈尔滨: 东北林业大学, 2007.
[17] Wang J.Studies on the allelopathy effects of Iva xanthifolia on organism growth. Harbin: Northeast Forestry University, 2012.
王晶. 假苍耳化感作用对生物生长发育的研究. 哈尔滨: 东北农业大学, 2012.
[18] Zu Y G, Jia J, Wang W J, et al. Changes in several physiological-biochemical parameters during the cycle of Iva xanthifolia. Chinese Bulletin of Botany, 2006, 23(4): 348-355.
祖元刚, 贾晶, 王文杰, 等. 假苍耳的生活史进程中几种生理生化指标的变化. 植物学报, 2006, 23(4): 348-355.
[19] Wang L J, Zhao X H, Yu J H, et al. Experiment study on Iva xanthifolia to feed young rabbit. Bulletin of Botanical Research, 2007, 27(5): 630-635.
王丽娟, 赵修华, 于景华, 等. 假苍耳喂饲幼兔试验研究. 植物研究, 2007, 27(5): 630-635.
[20] Zhao W, Tao B.Study on allelopathy effects of Iva xanthifolia on plants. Journal of Northeast Agricultural University, 2009, 40(4): 21-24.
赵微, 陶波. 外来杂草假苍耳(Iva xanthiifolia)化感作用研究. 东北农业大学学报, 2009, 40(4): 21-24.
[21] Bruce W G, Richardson D.Bioassays for allelopathy: measuring treatment responses with independent controls. Journal of Chemical Ecology, 1988, 14(1): 181-187.
[22] Liao Z Y, Zhao Z H, Hou Y P, et al. Allelopathic effects of Ipomoea cairica on Mikania micrantha. Ecology and Environmental Sciences, 2007, 16(3): 939-943.
廖周瑜, 赵则海, 侯玉平, 等. 五爪金龙对薇甘菊的化感效应研究. 生态环境学报, 2007, 16(3): 939-943.
[23] Huang Y M, Zhang Y X, Liu Q L, et al. Research on allelopathy of aqueous extract from Tagetes patula to four garden plants. Acta Prataculturae Sinica, 2015, 24(6): 150-158.
黄玉梅, 张杨雪, 刘庆林, 等. 孔雀草水浸提液对4种园林植物化感作用的研究. 草业学报, 2015, 24(6): 150-158.
[24] Hu Y B, Chen J, Xiao T H, et al. Research on allelopathy of aquous extract from Astragalus strictus. Acta Prataculturae Sinica, 2013, 22(6): 136-142.
胡远彬, 陈俊, 肖天昊, 等. 劲直黄芪水浸提液化感作用研究. 草业学报, 2013, 22(6): 136-142.
[25] Cao Z L, Wang X L, Tu J.Allelopathic effect of aqueous extracts of Eupatorium adenophorum Spreng by different treatment methods on seed germination of Pinus yunanensis Franch. Seed, 2011, 30(8): 46-49.
曹子林, 王晓丽, 涂璟. 紫茎泽兰不同处理方法水提液对云南松种子萌发的化感作用. 种子, 2011, 30(8): 46-49.
[26] Zhang R M, Wang Y Z, Hou P, et al. Physiological responses to allelopathy of aquatic stem and leaf extract of Artemisia frigida in seedling of several pasture plants. Acta Ecologica Sinica, 2010, 30(8): 2197-2204.
张汝民, 王玉芝, 侯平, 等. 几种牧草幼苗对冷蒿茎叶水浸提液化感作用的生理响应. 生态学报, 2010, 30(8): 2197-2204.
[27] Zheng L, Feng Y L.Allelopathic effects of Eupatorium adenophorum spreng on seed germination and seedling growth in ten herbaceous species. Acta Ecologica Sinica, 2005, 25(10): 2782-2787.
郑丽, 冯玉龙. 紫茎泽兰叶片化感作用对10种草本植物种子萌发和幼苗生长的影响. 生态学报, 2005, 25(10): 2782-2787.
[28] Liu C.Allelopathy of Phragmites australis and the isolation and identification of allelochemicals in P.australis. Chongqing: Southwest University, 2014.
刘成. 芦苇化感作用及其化感物质分离与鉴定. 重庆: 西南大学, 2014.
[29] Rudrappa T, Bonsall J, Gallagher J L, et al. Root-secreted allelochemical in the noxious weed Phragmites australis, deploys a reactive oxygen species response and microtubule assembly disruption to execute rhizotoxicity. Journal of Chemical Ecology, 2007, 33(10): 1898-1918.
[30] Wang B, Cai J, Jiang Z M, et al. Allelopathy effects of aqueous extracts of Juglans regia on four crops. Agricultural Research in the Arid Areas, 2011, 29(4): 47-52.
王蓓, 蔡靖, 姜在民, 等. 核桃叶水浸液对四种作物的化感作用. 干旱地区农业研究, 2011, 29(4): 47-52.