日粮添加葡萄渣提取物对湖羊外周血液LH、T、E2浓度的影响

doi:10.11686/cyxb2019228

草业学报 ›› 2020, Vol. 29 ›› Issue (2): 193-198.DOI: 10.11686/cyxb2019228

• 研究简报 • 上一篇

日粮添加葡萄渣提取物对湖羊外周血液LH、T、E2浓度的影响

韩秉成^1,**, 申琴^1,**, 韩阳阳¹, 朱浦嘉¹, 李万宏^1,2,3,*, 翁秀秀^1,2,3, 李发弟^1,2,3, 王超¹, 任芳¹

1.兰州大学草业科学国家级实验教学示范中心,兰州大学草地农业科技学院, 甘肃兰州730020;
2.草地农业生态系统国家重点实验室, 农业农村部草牧业创新重点实验室, 甘肃兰州 730020;
3.甘肃省肉羊繁育生物技术工程实验室, 甘肃民勤 733300

收稿日期:2019-04-09 修回日期:2019-09-16 出版日期:2020-02-20 发布日期:2020-02-20
通讯作者: E-mail: limh@lzu.edu.cn
作者简介:韩秉成(1997-),男,甘肃兰州人,在读本科。E-mail: hanbch16@lzu.edu.cn;申琴(1997-),女,贵州务川人,在读本科。E-mail: shenq2016@lzu.edu.cn。**共同第一作者These authors contributed equally to this work.
基金资助:
甘肃省农业生物技术研究与应用开发项目(GNSW-2016-22),兰州大学学生创新创业行动计划项目(201710730107,20171073001018),中央高校基本科研业务费(lzujbky-2019-39)和国家肉羊产业技术体系(CARS-38)资助

Effect of grape pomace extract on serum luteinizing hormone, testosterone and estradiol concentrations in Hu rams

HAN Bing-cheng^1,**, SHEN Qin^1,**, HAN Yang-yang¹, ZHU Pu-jia¹, LI Wan-hong^1,2,3,*, WENG Xiu-xiu^1,2,3, LI Fa-di^1,2,3, WANG Chao¹, REN Fang¹

1.National Demonstration Center for Experimental Grassland Science Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2.State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou 730020, China;
3.Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin, 733300, China

Received:2019-04-09 Revised:2019-09-16 Online:2020-02-20 Published:2020-02-20
Contact: E-mail: limh@lzu.edu.cn

摘要/Abstract

摘要： 为探讨日粮添加葡萄渣提取物对湖羊生殖相关激素促黄体素(LH)、睾酮(T)和雌二醇(E2)分泌的影响。选择24只体重(22.74±0.23) kg相近的3月龄湖羊公羔,随机分为3组,每组8只。对照组饲喂含有4%亚麻籽油的基础日粮,T₁和T₂组日粮分别添加0.36%和0.72%葡萄渣提取物。试验预饲期7 d,过渡期14 d,正式期60 d。结果表明:1)添加葡萄渣提取物对宰前活重影响不显著(P>0.05);2)与对照组相比,初情期前湖羊日粮添加亚麻籽油同时添加0.36%葡萄渣提取物对睾丸重量、睾丸体积、睾丸指数和附睾重量影响不显著(P>0.05),添加0.72%的葡萄渣提取物可显著提高睾丸重量[(233.02±32.67) g vs. (347.82±31.82) g,P<0.05]、睾丸体积[(255.50±40.55) mL vs. (365.63±32.41) mL,P<0.05]、睾丸指数[(0.61±0.10) vs. (0.92±0.09), P<0.05]和附睾重量[(45.53±4.00) g vs. (54.38±4.03) g,P=0.088],且T₁[(182.76±8.26) μm]、T₂[(220.25±6.69) μm]组睾丸曲细精管直径显著高于对照组[(160.02±7.55) μm,P<0.05];3)与对照组相比较,T₂组睾丸组织中促黄体素受体(LHR)、类固醇激素合成急性调节蛋白(StAR)基因表达显著上调(P<0.05),芳香化酶CYP19A1基因表达下调(P<0.05);4)T₂组外周血液T浓度显著高于对照组[(803.22±145.74) pg·mL^-1 vs. (575.09±57.58) pg·mL^-1, P<0.05],但是LH浓度下降[(0.05±0.01) IU·L^-1 vs. (0.11±0.03) IU·L^-1, P<0.05],T₁组各激素浓度与对照组相比,差异不显著(P>0.05)。综上所述,在本试验条件下,初情期前湖羊公羔日粮添加0.72%的葡萄渣提取物有利于羔羊睾丸和附睾发育。

关键词: 葡萄渣提取物, 生殖激素, 初情期, 湖羊

Abstract: This study investigated the effect of grape pomace extract (GPE) on luteinizing hormone (LH), testosterone (T) and estradiol (E2) concentration in serum of Hu rams. Twenty-four Hu rams weighing (22.74±0.23 kg) were randomly divided into three groups (n=8). A control group of rams were fed a basal diet containing 4% linseed oil, while T₁ and T₂ groups were fed the basal diet supplemented with 0.36% and 0.72% GPE, respectively. The experiment duration was 81 days, comprising a 7-day pre-trial period, a 14-day transition period, and a 60-day formal experiment. At the conclusion of the feeding experiment, the rams were slaughtered and relevant data collected. The results showed: 1) supplementation with GPE did not significantly affect body weight (P>0.05). 2) Compared with the control group, supplementation with 0.72% GPE in diets significantly increased testicular weight (233.02±32.67 g vs. 347.82±31.82 g, P<0.05), testicular volume (255.50±40.55 mL vs. 365.63±32.41 mL, P<0.05), a testicular size index (0.61±0.10 vs. 0.92±0.09, P<0.05), diameter of seminiferous tubules (160.02±7.55 μm vs. 220.25±6.69 μm, P<0.05) and epididymis weight (45.53±4.00 g vs. 54.38±4.03 g, P=0.088). However, there was no significant difference between T₁ and control groups (P>0.05), except for the diameter of seminiferous tubules (160.02±7.55 μm vs. 182.76±8.26 μm, P<0.05). 3) Compared with the control group, the expression of steroidogenic acute regulatory protein and luteinizing hormone receptor genes in group T₂ was significantly up-regulated (P<0.05), while the cytochrome P450 family 19 subfamily A member 1 expression was inhibited (P<0.05). 4) The concentration of testosterone in group T₂ was significantly higher than in the control group (803.22±145.74 pg·mL^-1 vs. 575.09±57.58 pg·mL^-1, P<0.05), while the concentration of LH in group T₂ was decreased (0.05±0.01 IU·L^-1 vs. 0.11±0.03 IU·L^-1, P<0.05). The T₁ treatment did not significantly affect LH, T and E2 concentrations (P>0.05). In conclusion, the supplementation of 0.72% GPE in diets improved testis development during peri-puberty by regulating testosterone synthesis in Hu rams.

Key words: grape pomace extract, reproductive hormones, puberty, Hu sheep

韩秉成, 申琴, 韩阳阳, 朱浦嘉, 李万宏, 翁秀秀, 李发弟, 王超, 任芳. 日粮添加葡萄渣提取物对湖羊外周血液LH、T、E2浓度的影响[J]. 草业学报, 2020, 29(2): 193-198.

HAN Bing-cheng, SHEN Qin, HAN Yang-yang, ZHU Pu-jia, LI Wan-hong, WENG Xiu-xiu, LI Fa-di, WANG Chao, REN Fang. Effect of grape pomace extract on serum luteinizing hormone, testosterone and estradiol concentrations in Hu rams[J]. Acta Prataculturae Sinica, 2020, 29(2): 193-198.

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日粮添加葡萄渣提取物对湖羊外周血液LH、T、E2浓度的影响

Effect of grape pomace extract on serum luteinizing hormone, testosterone and estradiol concentrations in Hu rams

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