氮素添加对贝加尔针茅草原土壤团聚体碳、氮和磷生态化学计量学特征的影响

doi:10.11686/cyxb2019297

摘要/Abstract

摘要： 大气氮沉降增加作为全球气候变化的重要现象, 其对草原生态系统的影响成为生态学研究热点之一。掌握氮沉降对草原土壤团聚体碳(C)、氮(N)和磷(P)生态化学计量学特征的影响,可为全面分析和评估氮沉降对草原生态系统的影响提供基础资料。自2010年起,在内蒙古贝加尔针茅草原典型地段设置N₀ (0 kg N·hm^-2·yr^-1)、N₁₅ (15 kg N·hm^-2·yr^-1)、N₃₀ (30 kg N·hm^-2·yr^-1)、N₅₀ (50 kg N·hm^-2·yr^-1)、N₁₀₀ (100 kg N·hm^-2·yr^-1)、N₁₅₀ (150 kg N·hm^-2·yr^-1) 6个氮素添加处理模拟氮沉降野外控制试验。结果表明:氮素添加极显著提高了土壤团聚体的稳定性和>2 mm团聚体比例(P<0.01);各氮素添加处理中0.25~2 mm团聚体有机碳、全氮含量均显著高于其他粒径(P<0.05),全磷含量在各粒径团聚体中差异不显著;与对照相比,氮素添加显著提高了>0.25 mm土壤大团聚体有机碳和全氮含量(P<0.05),对全磷无显著影响;氮素添加导致>0.25 mm土壤大团聚体C/N降低,0.25~2 mm土壤团聚体 C/P、N/P升高(P<0.05)。综合分析,氮添加在一定程度上促进了土壤的固碳潜力,提高了土壤团聚体有机质的矿化速率,随着氮素添加水平的提高,土壤团聚体中P元素成为限制草原植物生长的主要限制因子。

关键词: 土壤团聚体, 生态化学计量特征, 氮沉降, 贝加尔针茅草原

Abstract: The influence of atmospheric nitrogen deposition on grassland ecosystems is an important component process of global climate change, and therefore has become a research hotspot in ecology. Our study aimed to understand the influence of nitrogen deposition on the carbon (C), nitrogen (N) and phosphorus (P) ecological stoichiometric characteristics of grassland soil aggregates, in order to provide fundamental research data for the comprehensive analysis and assessment of the impact of nitrogen deposition on grassland ecosystems. Since 2010, six simulated nitrogen deposition field control experiments of N₀ (0 kg N·ha^-1·yr^-1), N₁₅ (15 kg N·ha^-1·yr^-1), N₃₀ (30 kg N·ha^-1·yr^-1), N₅₀ (50 kg N·ha^-1·yr^-1), N₁₀₀ (100 kg N·ha^-1·yr^-1) and N₁₅₀ (150 kg N·ha^-1·yr^-1) were set up in an area of typical Stipa baicalensis grassland in Inner Mongolia. It was found that nitrogen addition significantly (P<0.01) increased the stability of soil aggregates and the proportion of aggregates >2 mm in diameter. In addition, the organic carbon and total nitrogen contents of 0.25-2 mm aggregates were significantly (P<0.05) higher than those of other particle sizes, but the total phosphorus contents did not differ significantly with different particle sizes. Compared with the control group, nitrogen addition significantly (P<0.05) increased the organic carbon and total nitrogen content of soil macroaggregates >0.25 mm diameter, but had no significant effect on total phosphorus. Nitrogen addition reduced the C∶N ratio of >0.25 mm aggregates, but increased C∶P and N∶P ratios of 0.25-2 mm aggregates (P<0.05). A comprehensive analysis shows that nitrogen addition promotes the carbon sequestration potential of soil to a certain degree and increases the mineralization rate of organic matter in soil aggregates. With the increase of nitrogen addition level, the elemental P level in soil aggregates becomes the main limiting factor restricting the growth of grassland plants.

Key words: soil aggregates, ecological stoichiometric characteristics, nitrogen deposition, Stipa baicalensis grassland

李明, 秦洁, 红雨, 杨殿林, 周广帆, 王宇, 王丽娟. 氮素添加对贝加尔针茅草原土壤团聚体碳、氮和磷生态化学计量学特征的影响[J]. 草业学报, 2019, 28(12): 29-40.

LI Ming, QIN Jie, HONG Yu, YANG Dian-lin, ZHOU Guang-fan, WANG Yu, WANG Li-juan. Effects of nitrogen addition on ecological stoichiometric characteristics of carbon, nitrogen and phosphorus in Stipa baicalensis grassland soil aggregates[J]. Acta Prataculturae Sinica, 2019, 28(12): 29-40.

参考文献

[1] Chen H, Mo J M, Zhang W, et al. The effects of nitrogen deposition on forest carbon sequestration: A review. Acta Ecologica Sinica, 2012, 32(21): 6864-6879.
陈浩, 莫江明, 张炜, 等. 氮沉降对森林生态系统碳吸存的影响. 生态学报, 2012, 32(21): 6864-6879.
[2] Holland E A, Dentener F J, Braswell B H, et al. Contemporary and pre-industrial global reactive nitrogen budgets. Biogeochemistry, 1999, 46: 7-43.
[3] Guo R Q, Xiong D C, Song T T, et al. Effects of simulated nitrogen deposition on stoichiometry of fineroots of Chinese fir (Cunninghamia lanceolata) seedlings. Acta Ecologica Sinica, 2018, 38(17): 6101-6110.
郭润泉, 熊德成, 宋涛涛, 等. 模拟氮沉降对杉木幼苗细根化学计量学特征的影响. 生态学报, 2018, 38(17): 6101-6110.
[4] Chen J, Cao J J, Liu Y, et al. Research progress and prospect of nitrogen fertilization on soil respiration. Grassland and Turf, 2013, 33(6): 87-93.
陈骥, 曹军骥, 刘玉, 等. 氮素添加对土壤呼吸影响的研究进展. 草原与草坪, 2013, 33(6): 87-93.
[5] Mo J M, Xue J H, Fang Y T.Litter decomposition and its responses to simulated N deposition for the major plants of Dinghushan forests in subtropical China. Acta Ecologica Sinica, 2004, 24(7): 1413-1420.
莫江明, 薛璟花, 方运霆. 鼎湖山主要森林植物凋落物分解及其对N沉降的响应. 生态学报, 2004, 24(7): 1413-1420.
[6] Bergstrom A, Jansson M.Atmospheric nitrogen deposition has caused nitrogen enrichment and eutrophication of lakes in the northern hemisphere. Global Change Biology, 2006, 12(4): 635-643.
[7] Sun Z G, Sun C M, Li J L, et al. Retrospect and prospect of carbon circle mechanism and carbon storage calculation of grassland ecosystem in China. Pratacultural Science, 2011, 28(9): 1611-1616.
孙政国, 孙成明, 李建龙, 等. 我国草地生态系统碳循环机制及碳蓄积核算研究回顾与展望. 草业科学, 2011, 28(9): 1611-1616.
[8] Zhang X S, Tang H P, Dong X B, et al. The dilemma of steppe and it’s transformation in China. Chinese Science Bulletin, 2016, 61(2): 165-177.
张新时, 唐海萍, 董孝斌, 等. 中国草原的困境及其转型. 科学通报, 2016, 61(2): 165-177.
[9] Xu X, Cheng Y W, Jiang H L, et al. Research progress of the effects of wind speed change on grassland ecosystem. Acta Ecologica Sinica, 2017, 37(12): 4289-4298.
徐霞, 成亚薇, 江红蕾, 等. 风速变化对草原生态系统的影响研究进展. 生态学报, 2017, 37(12): 4289-4298.
[10] Wang X J, Zhang L M.Investigation report about Hulunbeir grassland desertification. Forestry Economics, 2006, (7): 8-10.
王信建, 张利明. 关于呼伦贝尔草原沙化情况的调查报告. 林业经济, 2006, (7): 8-10.
[11] Hou X Y.Developing grassland ecological animal husbandry is the effective way to settle the dilemma of grassland degradation. Chinese Journal of Grassland, 2010, 32(4): 3-11.
侯向阳. 发展草原生态畜牧业是解决草原退化困境的有效途径. 中国草地学报, 2010, 32(4): 3-11.
[12] Zhang C H, Zhang L M, Liu X R, et al. Root tissue and shoot litter decomposition of dominant species Stipa baicalensis in Hulun Buir meadow steppe of Inner Mongolia, China. Chinese Journal of Plant Ecology, 2011, 35(11): 1156-1166.
张彩虹, 张雷明, 刘杏认, 等. 呼伦贝尔草甸草原优势种贝加尔针茅根系组织和地上部分凋落物的分解. 植物生态学报, 2011, 35(11): 1156-1166.
[13] Zhu L X, Ma S M, Wang Z F.Soil eco-geochemical baseline in alluvial plains of eastern China. Geology in China, 2006, 33(6): 1400-1405.
朱立新, 马生明, 王之峰. 中国东部平原土壤生态地球化学基准值. 中国地质, 2006, 33(6): 1400-1405.
[14] Yang C, Liu N, Zhang Y J.Soil aggregates regulate the impact of soil bacterial and fungal communities on soil respiration. Geoderma, 2019, 337: 444-452.
[15] Li R, Jiang C S, Hao Q J.Impact of land utilization pattern on distributing characters of labile organic carbon in soil aggregates in Jinyun mountain. Environmental Science, 2015, 36(9): 3429-3437.
李睿, 江长胜, 郝庆菊. 缙云山不同土地利用方式下土壤团聚体中活性有机碳分布特征. 环境科学, 2015, 36(9): 3429-3437.
[16] Rahman M T, Zhu Q H, Zhang Z B, et al. The roles of organic amendments and microbial community in the improvement of soil structure of a vertisol. Applied Soil Ecology, 2017, 111: 84-93.
[17] Bodlák L, Krováková K, Kobesová M, et al. SOC content-An appropriate tool for evaluating the soil quality in a reclaimed post-mining landscape. Ecological Engineering, 2012, 43: 53-59.
[18] Lal R, Kimble J M.Conservation tillage for carbon sequestration. Nutrient Cycling in Agroecosystems, 1997, 49(1): 243-253.
[19] Angélica L G, John S K, Michael D, et al. Can ecological stoichiometry help explain patterns of biological invasions. Oikos, 2010, 119(5): 779-790.
[20] He J S, Han X G.Ecological stoichiometry: Searching for unifying principles from individuals to ecosystems. Chinese Journal of Plant Ecology, 2010, 34(1): 2-6.
贺金生, 韩兴国. 生态化学计量学: 探索从个体到生态系统的统一化理论. 植物生态学报, 2010, 34(1): 2-6.
[21] He H L, Yang X C, Wang D, et al. Ecological stoichiometric characteristics of soil carbon, nitrogen and phosphorus of Sibiraea angustata shrub in eastern Qinghai-Tibetan Plateau. Chinese Journal of Applied and Environmental Biology, 2015, 21(6): 1128-1135.
贺合亮, 阳小成, 王东, 等. 青藏高原东部窄叶鲜卑花灌丛土壤C、N、P生态化学计量学特征. 应用与环境生物学报, 2015, 21(6): 1128-1135.
[22] Yin X R, Liang C Z, Wang L X, et al. Ecological stoichiometry of plant nutrients at different restoration succession stages in typical steppe of Inner Mongolia, China. Chinese Journal of Plant Ecology, 2010, 34(1): 39-47.
银晓瑞, 梁存柱, 王立新, 等. 内蒙古典型草原不同恢复演替阶段植物养分化学计量学. 植物生态学报, 2010, 34(1): 39-47.
[23] Zhang H F, Liu H M, Zhao J N, et al. Response of soil fungal community structure to nitrogen and water addition in Stipa baicalensis steppe. Acta Ecologica Sinica, 2018, 38(1): 195-205.
张海芳, 刘红梅, 赵建宁, 等. 贝加尔针茅草原土壤真菌群落结构对氮素和水分添加的响应. 生态学报, 2018, 38(1): 195-205.
[24] Liu H M, Zhou G F, Li J, et al. Effects of nitrogen deposition on soil enzyme activities of Stipa baicalensis steppe. Ecology and Environmental Sciences, 2018, 27(8): 1387-1394.
刘红梅, 周广帆, 李洁, 等. 氮沉降对贝加尔针茅草原土壤酶活性的影响. 生态环境学报, 2018, 27(8): 1387-1394.
[25] Liu J, Zhang Y W, Zhang Q M, et al. Effect of different land use types on soil aggregates microbial biomass and community composition in the Loess Hilly region of west Henan. Pratacultural Science, 2018, 35(4): 771-780.
刘晶, 张跃伟, 张巧明, 等. 土地利用方式对豫西黄土丘陵区土壤团聚体微生物生物量及群落组成的影响. 草业科学, 2018, 35(4): 771-780.
[26] Guo J H, Chen X Y, Liu M Q, et al. Effects of fertilizer management practice on distribution of aggregates and content of organic carbon and nitrogen in red paddy soil. Soils, 2007, 39(5): 787-793.
郭菊花, 陈小云, 刘满强, 等. 不同施肥处理对红壤性水稻土团聚体的分布及有机碳、氮含量的影响. 土壤, 2007, 39(5): 787-793.
[27] Qi X X, Wang H Y, Lin F, et al. Effect of Flaveria bidentis invasion on the community composition and performance of native plants grown in invaded soil. Acta Ecologica Sinica, 2019, 39(12): 4463-4477.
祁小旭, 王红岩, 林峰, 等. 黄顶菊对入侵地群落动态及植物生长生理特征的影响. 生态学报, 2019, 39(12): 4463-4477.
[28] Six J, Bossuyt H, Degryze D, et al. A history of research on the link between (micro) aggregates, soil biota, and soil organic matter dynamics. Soil & Tillage Research, 2004, 79(1): 7-31.
[29] Goulet E, Dousset S, Chaussod R, et al. Water-stable aggregates and organic matter pools in a calcareous vineyard soil under four soil-surface management systems. Soil Use and Management, 2004, 20(3): 318-324.
[30] Xing W, Zhao H M, Xu Y, et al. Effect of short-term nitrogen addition on topsoil water-stable aggregate in poplar plantation. Guizhou Agricultural Sciences, 2014, 42(12): 129-133.
邢玮, 赵慧敏, 徐钰, 等. 短期氮添加对杨树人工林土壤表层水稳态团聚体的影响. 贵州农业科学, 2014, 42(12): 129-133.
[31] Guo H B, Yuan Y H, Wu J P, et al. Distribution of water-stable aggregates and organic carbon in response to simulated nitrogen deposition in a Chinese fir plantation. Journal of Soil and Water Conservation, 2013, 27(4): 268-272.
郭虎波, 袁颖红, 吴建平, 等. 氮沉降对杉木人工林土壤团聚体及其有机碳分布的影响. 水土保持学报, 2013, 27(4): 268-272.
[32] Liu H M, Li J, Wang L L, et al. Effects of nitrogen addition on the stoichiometric characteristics of plants and soil in the Stipa baicalensis grassland of Inner Mongolia, China. Acta Prataculturae Sinica, 2018, 27(7): 25-35.
刘红梅, 李洁, 王丽丽, 等. 氮添加对贝加尔针茅草原植物和土壤化学计量特征的影响. 草业学报, 2018, 27(7): 25-35.
[33] Liu X D, Yin G L, Wu J, et al. Effects of nitrogen addition on the physical properties of soil in an alpine meadow on the eastern Qinghai-Tibetan Plateau. Acta Prataculturae Sinica, 2015, 24(10): 12-21.
刘晓东, 尹国丽, 武均, 等. 青藏高原东部高寒草甸草地土壤物理性状对氮元素添加的响应. 草业学报, 2015, 24(10): 12-21.
[34] Qi Y, Mulder J, Duan L, et al. Short-term effects of simulating nitrogen deposition on soil organic carbon in a Stipa krylovii steppe. Acta Ecologica Sinica, 2015, 35(4): 1104-1113.
祁瑜, Mulder J, 段雷, 等. 模拟氮沉降对克氏针茅草原土壤有机碳的短期影响. 生态学报, 2015, 35(4): 1104-1113.
[35] Fornara D A, Tilman D.Soil carbon sequestration in prairie grasslands increased by chronic nitrogen addition. Ecological Society of America, 2012, 93(9): 2030-2036.
[36] Li W, Zhang Z C, Li T X.Ecological stoichiometry of soil carbon,nitrogen and phosphorus within soil aggregates in tea plantations with different ages. Chinese Journal of Applied Ecology, 2015, 26(1): 9-16.
李玮, 郑子成, 李廷轩. 不同植茶年限土壤团聚体碳氮磷生态化学计量学特征. 应用生态学报, 2015, 26(1): 9-16.
[37] Zhu Q L, Wang C, Tong C, et al. Effects of the waste and grade on the ecological stoichiometric ratios of soil aggregates in paddy field. Research and Exploration in Laboratory, 2016, 35(11): 16-21.
朱秋丽, 王纯, 仝川, 等. 废弃物与粒级对土壤团聚体生态化学计量比的影响. 实验室研究与探索, 2016, 35(11): 16-21.
[38] Zhang H F.Responses of plant and soil microbial communities to nitrogen and water addition on the Stipa baicalensis steppe. Beijing: Chinese Academy of Agricultural Sciences, 2017.
张海芳. 贝加尔针茅草原植物与土壤微生物群落对氮素和水分添加的响应. 北京: 中国农业科学院, 2017.
[39] Li R R, Lu Y, Wang Y M, et al. Effects of N addition on C,N and P stoichiometry and soil enzyme activities in Cupressuslusitanica Mill. plantation. Chinese Journal of Ecology, 2019, 38(2): 384-393.
李瑞瑞, 卢艺, 王益明, 等. 氮添加对墨西哥柏人工林土壤碳氮磷化学计量特征及酶活性的影响. 生态学杂志, 2019, 38(2): 384-393.
[40] Yang K, Zhu J J, Gu J C, et al. Changes in soil phosphorus fractions after 9 years of continuous nitrogen addition in a Larix gmelinii plantation. Annals of Forest Science, 2015, 72: 435-442.
[41] Sun J, Zhao F Z, Han X H, et al. Ecological stoichiometry of soil aggregates and relationship with soil nutrients of different-aged Robinia pseudoacacia forests. Acta Ecologica Sinica, 2016, 36(21): 6879-6888.
孙娇, 赵发珠, 韩新辉, 等. 不同林龄刺槐林土壤团聚体化学计量特征及其与土壤养分的关系. 生态学报, 2016, 36(21): 6879-6888.
[42] Scott D, Daniel M, Paul H, et al. Soil aggregates and associated organic matter under conventional tillage, no-tillage, and forest succession after three decades. PLoS One, 2014, 9(1): 1-12.
[43] Maysoon M M, Charles W R.Tillage and manure effects on soil and aggregate-associated carbon and nitrogen. Soil Science Society of America Journal, 2004, 68(3): 809-816.
[44] Kong Y G, Zhang J C, Zhang D H, et al. Effects of land-use change on soil and aggregate-associated organic carbon. Journal of Central South University of Forestry & Technology, 2009, 29(2): 39-44.
孔雨光, 张金池, 张东海, 等. 土地利用变化对土壤及团聚体结合有机碳的影响. 中南林业科技大学学报, 2009, 29(2): 39-44.
[45] Xie J S, Yang Y S, Xie M S, et al. Effect of vegetation restoration on soil organic matter of light fraction in eroded degraded red soil in subtropics of China. Acta Pedologica Sinica, 2008, 45(1): 170-175.
谢锦升, 杨玉盛, 解明曙, 等. 植被恢复对退化红壤轻组有机质的影响. 土壤学报, 2008, 45(1): 170-175.
[46] Li Q J, Xue Z J, Zhou Z C.Effects of vegetation restoration on nutrient and microbial properties of soil aggregates with different particle sizes in the loess hilly regions of Ningxia, Northwest China. Chinese Journal of Applied Ecology, 2019, 30(1): 137-145.
李秋嘉, 薛志婧, 周正朝. 宁南山区植被恢复对土壤团聚体养分特征及微生物特性的影响. 应用生态学报, 2019, 30(1): 137-145.
[47] Liu Y J, Ni J P, Zhang Y, et al. Effects of different crop-mulberry intercropping systems on nutrients in arid purple soils in the Three Gorges Reservoir Area. Acta Prataculturae Sinica, 2015, 24(12): 38-45.
刘月娇, 倪九派, 张洋, 等. 三峡库区紫色土旱坡地农桑配置模式对土壤养分的影响. 草业学报, 2015, 24(12): 38-45.
[48] Zhang C H, Wang Z M, Ju W M, et al. Spatial and temporal variability of soil C/N ratio in Songnen plain maize belt. Environmental Science, 2011, 32(5): 1407-1414.
张春华, 王宗明, 居为民, 等. 松嫩平原玉米带土壤碳氮比的时空变异特征. 环境科学, 2011, 32(5): 1407-1414.
[49] An S, Mentler A, Mayer H, et al. Soil aggregation, aggregate stability, organic carbon and nitrogen in different soil aggregate fractions under forest and shrub vegetation on the Loess Plateau, China. Catena, 2010, 81(3): 226-233.
[50] Liptzin C D.C∶N∶P stoichiometry in soil: Is there a “Redfield ratio” for the microbial biomass? Biogeochemistry, 2007, 85(3): 235-252.
[51] Chen L, Zhu G Y, Liu Y L, et al. Responses of soil carbon and nitrogen to short term nitrogen addition in Pinus tabulae formis carriere forest on the Loess Plateau. Journal of Soil and Water Conservation, 2018, 32(4): 346-352.
陈磊, 朱广宇, 刘玉林, 等. 黄土高原人工油松林土壤碳氮对短期氮添加的响应. 水土保持学报, 2018, 32(4): 346-352.