细叶百合LpWRKY20基因对非生物胁迫的响应及抗旱性分析

doi:10.11686/cyxb2019318

摘要/Abstract

摘要： 以细叶百合LpWRKY20基因为研究对象,通过荧光定量(qRT-PCR)分析该基因在不同非生物胁迫中的表达,结果表明,在不同非生物胁迫中该基因表达量存在差异。利用叶盘法将pBI121-LpWRKY20-GFP植物表达载体转入烟草并获得转基因株系。在干旱胁迫条件下,转基因植株的表型优于野生型;转基因植株超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性均高于野生型,且随着时间的延长这种清除体内自由基的能力在显著升高,而丙二醛(MDA)含量均低于野生型,说明转基因植株细胞膜受损程度较低,具有较强的自我修复能力。干旱条件下表型及生理指标的变化均表明转基因植株具有较强的抗旱性,初步推断LpWRKY20具有抗旱的功能。

关键词: 细叶百合, LpWRKY20, 非生物胁迫, 遗传转化, 抗旱性

Abstract: This research investigated the expression of the LpWRKY20 gene under different abiotic stresses, using quantitative reverse transcription PCR (qRT-PCR). The plant expression vector pBI121-LpWRKY20-GFP was transferred into tobacco by the leaf disc method and transgenic lines were obtained. The expression of this gene differed under different abiotic stresses. The phenotype of transgenic plants was better than that of wild plants under drought stress. The activities of superoxide dismutase and catalase of transgenic plants were higher than those of the wild type, and the ability to remove free radicals in vivo increased significantly over time. Tissue malondialdehyde level was lower than that of the wild type, indicating that the cell membrane of transgenic plants was less damaged and had a strong self-repairing ability. The change of phenotype and physiological indexes under drought conditions indicated that transgenic plants had strong drought resistance, and the preliminary conclusion was reached that the LpWRKY20 gene confers drought resistance.

Key words: Lilium pumilum, LpWRKY20, abiotic stress, genetic transformation, drought resistance

杨柳慧, 尹航, 黄沁梅, 张彦妮, 何淼, 周蕴薇. 细叶百合LpWRKY20基因对非生物胁迫的响应及抗旱性分析[J]. 草业学报, 2020, 29(1): 193-202.

YANG Liu-hui, YIN Hang, HUANG Qin-mei, ZHANG Yan-ni, HE Miao, ZHOU Yun-wei. An analysis of the response of the LpWRKY20 gene to abiotic stress and its role in drought resistance[J]. Acta Prataculturae Sinica, 2020, 29(1): 193-202.

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