Kunitz型胰蛋白酶抑制剂缺失大豆新品系培育及其饲草价值分析

doi:10.11686/cyxb2019547

草业学报 ›› 2020, Vol. 29 ›› Issue (10): 91-98.DOI: 10.11686/cyxb2019547

Kunitz型胰蛋白酶抑制剂缺失大豆新品系培育及其饲草价值分析

姜妍^1,2, 薛恩玉³, 鹿文成⁴, 崔国文⁵, 李远明^1,2, 韩天富⁶, 王绍东^1,2,*

1.东北农业大学农学院,大豆生物学教育部重点实验室,黑龙江哈尔滨 150030;
2.黑龙江省绿色食品科学研究院,国家大豆工程技术研究中心,黑龙江哈尔滨 150028;
3.黑龙江省绿色食品发展中心,黑龙江哈尔滨 150036;
4.黑龙江省农业科学院黑河分院,黑龙江黑河 164300;
5.东北农业大学动物科技学院,黑龙江哈尔滨 150030;
6.中国农业科学院作物科学研究所,北京 100081

收稿日期:2019-12-16 出版日期:2020-10-20 发布日期:2020-10-20
通讯作者: *.E-mail: wsdhlj@neau.edu.cn
作者简介:姜妍(1979-),女,吉林辽源人,副研究员,博士。E-mail: yanjiang416@163.com
基金资助:
科技部“十三五”七大作物育种专项(2017YFD0101306-05)和国家大豆良种重大科研联合攻关项目(19190479)资助

Breeding and feeding quality analysis of a new soybean strain deficient in Kunitz trypsin inhibitor

JIANG Yan^1,2, XUE En-yu³, LU Wen-cheng⁴, CUI Guo-wen⁵, LI Yuan-ming^1,2, HAN Tian-fu⁶, WANG Shao-dong^1,2,*

1. College of Agriculture, Key Laboratory of Soybean Biology Ministry of Education, Northeast Agricultural University, Harbin 150030, China;
2. Heilongjiang Green Food Science Institute, National Soybean Engineering Technology Research Center, Northeast Agricultural University, Harbin 150028, China;
3. Heilongjiang Green Food development Center, Harbin 150036, China;
4. Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe 164300, China;
5. Animal Science and Techonology college, Northeast Agricultural University, Harbin 150030, China;
6. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2019-12-16 Online:2020-10-20 Published:2020-10-20
Contact: *.E-mail: wsdhlj@neau.edu.cn

摘要/Abstract

摘要： 大豆作为饲料蛋白质的重要来源,既含有丰富的营养成分,也有影响蛋白质消化吸收的胰蛋白酶抑制剂等抗营养因子。本研究以合丰50为母本,中黄16为父本,通过常规杂交育种及非变性聚丙烯酰胺凝胶电泳的分子辅助育种技术,经过多年选育,获得适合黑龙江省栽培的Kunitz型胰蛋白酶抑制剂缺失稳定遗传的大豆新品系“HZ8009”。该品系生育期120 d,植株高大茎叶繁茂,无限结荚习性,籽粒蛋白质含量42%,脂肪含量20%。利用饲草质量检测技术对高代品系“HZ7009”植株饲草营养价值分析表明,与牡丹江秣食豆相比,“HZ7009”干草产量高21.39%,干物质中粗蛋白质含量高3.92%,且最佳刈割时期为花期至结荚期。大豆新品系“HZ8009”具有高蛋白、高生物量的特性,可作饲草资源品种加以广泛利用。

关键词: 大豆, Kunitz型胰蛋白酶抑制剂, 营养价值

Abstract: As a source of feed protein, soybean contains rich nutritional components, but also have trypsin inhibitors that affect protein digestibility and absorption. Through conventional breeding and Native-PAGE molecular assisted breeding technology, a new soybean strain ‘HZ8009’ deficient in Kunitz type trypsin inhibitors was selected, with Hefeng50 as the female parent and Zhonghuang16 as the male parent. The new soybean strain HZ8009 is suitable for cultivation in Heilongjiang Province and has a maturity time of 120 days. The plant is tall with luxuriant foliage and an indeterminate podding habit. The protein content of the seed is 42%, and the fat content is 20%. The feed nutritional value of advanced generation strain was analyzed using feed quality testing technology. Hay yield was 21.39% greater and the dry matter crude protein content was 3.92% greater than for Mudanjiang fodder soybean. The best cutting period was from flowering to podding. The new soybean strain HZ8009 has a high protein content and high crop biomass, indicating wide potential for use as a forage resource.

Key words: soybean, Kunitz trypsin inhibitor, nutritive value

姜妍, 薛恩玉, 鹿文成, 崔国文, 李远明, 韩天富, 王绍东. Kunitz型胰蛋白酶抑制剂缺失大豆新品系培育及其饲草价值分析[J]. 草业学报, 2020, 29(10): 91-98.

JIANG Yan, XUE En-yu, LU Wen-cheng, CUI Guo-wen, LI Yuan-ming, HAN Tian-fu, WANG Shao-dong. Breeding and feeding quality analysis of a new soybean strain deficient in Kunitz trypsin inhibitor[J]. Acta Prataculturae Sinica, 2020, 29(10): 91-98.

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Kunitz型胰蛋白酶抑制剂缺失大豆新品系培育及其饲草价值分析

Breeding and feeding quality analysis of a new soybean strain deficient in Kunitz trypsin inhibitor

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