藏系绵羊瘤胃细菌数量及其放牧地牧草养分随季节变化的研究

草业学报 ›› 2009, Vol. 18 ›› Issue (1): 100-104.

藏系绵羊瘤胃细菌数量及其放牧地牧草养分随季节变化的研究

淡瑞芳¹,张海涛¹,龙瑞军^2,3*,丁学智⁴,张欣⁵

1.江苏畜牧兽医职业技术学院,江苏泰州225300;
2.兰州大学青藏高原生态系统管理国际中心,甘肃兰州730020;
3.兰州大学草地农业

收稿日期:2008-05-09 出版日期:2009-01-25 发布日期:2009-02-20
作者简介:淡瑞芳(1974-),女,山西河曲人,讲师,博士。E-mail:danreifang@126.com
基金资助:
IAEA/FAO(CPR12666/RO)资助。

Seasonal shift of rumen bacteria quantity of grazing Tibetan sheep and forage nutrition by grazing sheep

DAN Rui-fang¹, ZHANG Hai-tao¹, LONG Rui-jun^2,3, DING Xue-zhi⁴, ZHANG Xin⁵

1.Jiangsu Animal Husbandry &Veterinary College, Taizhou 225300, China;
2.International Centre for Tibetan Ecosystem Management, Lanzhou University, Lanzhou 730020, China;
3.College of Pastoral
Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
4.Northwest
Plateau Institute of Biology, Chinese Academy of Science, Xining 810001, China;
5.Department
of Basic Science, Tianjin Agricultural University, Tianjin 300384, China

Received:2008-05-09 Online:2009-01-25 Published:2009-02-20

摘要/Abstract

摘要： 试验分别于春、夏、秋和冬季,随机选择3只同群常年放牧健康的藏系绵羯羊(2岁),屠宰后采集瘤胃内容物。采用瘤胃细菌通用引物和实时定量PCR技术,研究瘤胃细菌数量随季节的动态变化。结果表明,1)夏季瘤胃细菌数量显著(P＜0.05)高于其他3个季节,分别是春季、秋季、冬季细菌数量的5.56,2.35和2.49倍;秋、冬季是春季细菌数量的2.38和2.23倍;而秋和冬季瘤胃细菌的数量差异不显著(P＞0.05)。2)夏季藏系绵羊采食量分别为春季、冬季和秋季的1.98,1.61和1.80倍,饲料中的纤维性物质含量增高,采食量也随着降低,瘤胃细菌的数量也相应降低;3)实时定量PCR技术能准确的反映出放牧藏系绵羊瘤胃细菌数量随季节的动态变化。

Abstract: In this study, real time PCR were used to monitor changed quantity of bacteria in rumens of 12 Tibetan sheep with seasonal shift, by collecting rumen content of grazing Tibetan sheep in alpine meadow in spring, summer, autumn and winter, respectively. 1)The quantity of bacteria in grazing Tibetan sheep rumen did not have a significantly change between winter and autumn, and that of spring decreased 2.38, 2.23-fold of autumn and winter, meanwhile, that of summer was significantly higher 5.56-fold, 2.35-fold and 2.49-fold than that of autumn, winter and spring;2)Intake of the grazing sheep in summer was significantly higher 1.98-fold, 1.61-fold and 1.80-fold than that of spring, winter, autumn. Intake of the grazing sheep and quantity of bacteria in rumens of the grazing sheep were decreased with fibre increased of fodder. The quantity of bacteria in rumens of Tibetan sheep changed, which result from fodder, season shift. 3)Real time PCR validated for specific detection and quantification of bacteria in rumens of Tibetan sheep with seasonal shift.

中图分类号:

S826

淡瑞芳,张海涛,龙瑞军,丁学智,张欣. 藏系绵羊瘤胃细菌数量及其放牧地牧草养分随季节变化的研究[J]. 草业学报, 2009, 18(1): 100-104.

DAN Rui-fang, ZHANG Hai-tao, LONG Rui-jun, DING Xue-zhi, ZHANG Xin. Seasonal shift of rumen bacteria quantity of grazing Tibetan sheep and forage nutrition by grazing sheep[J]. Acta Prataculturae Sinica, 2009, 18(1): 100-104.

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