玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析

草业学报 ›› 2009, Vol. 18 ›› Issue (3): 126-135.

玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析

柴兆祥^1,2,3,4,李金花^1,2,楼兵干⁵,李唯^6*,甘辉林¹,郭成¹,赵玲¹,董迪¹

1.甘肃农业大学草业学院,甘肃兰州 730070;
2.甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州 730070;
3.草业生态系统教育部重点
实验室,甘肃兰州 730070;
4.中-美草地畜牧业可持续发展研究中心,甘肃兰州 730070;
5.浙江大学生物技术研究所,
浙江杭州 310029;
6.甘肃农业大学生命科学技术学院,甘肃兰州 730070

收稿日期:2008-08-16 出版日期:2009-06-20 发布日期:2009-06-20
作者简介:柴兆祥(1968-),男,甘肃通渭人,教授,在职博士。E-mail:chaizx@yeah.net
基金资助:
甘肃省科学事业费项目(甘科计]2001]21号)和甘肃省教育厅项目(032-08)资助。

Isolation, identification, and sequence analysis of rDNA-ITS of
Pythium species sampled from rhizosphere soil of corn

CHAI Zhao-xiang^1,2,3,4, LI Jin-hua^1,2, LOU Bing-gan⁵, LI Wei⁶, GAN Hui-lin1,
GUO Cheng¹, ZHAO Ling¹, DONG Di¹

1.College of Grassland Sciences, Gansu Agricultural University, Lanzhou 730070, China;
2.Gansu Key
Lab of Crop improvement and Germplasm Enhancement, Lanzhou 730070, China;
3.Key Laboratory
of Grassland Ecosystem, Ministry of Education, Lanzhou 730070, China;
4.Sino-U.S. Centers for
Grazingland Ecosystem Sustainability, Lanzhou 730070, China;
5.Institute of Biotechnology,
Zhejiang University, Hangzhou 310029, China;
6.College of Life Science and
Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2008-08-16 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 从兰州地区的红古、七里河等地采集玉米根围土壤标样,诱饵法与组织分离法相结合分离纯化到23株腐霉菌株,通过形态特征、培养特性、生长速度和对温度适应范围的研究,结合rDNA-ITS序列分析,将分离到的腐霉菌株鉴定为硬腐霉、终极腐霉和一个腐霉待定种。这是首次报道在我国分离到硬腐霉,为我国新记录种;终极腐霉也系首次在甘肃从玉米根围土壤中分离到。统计表明,3种腐霉菌种在兰州地区玉米根围土壤中的分离频率不同,终极腐霉出现次数最多,为优势种群,分离频率为60.9%;其次为腐霉待定种,分离频率为26.1%;而硬腐霉分离频率最低,为13.0%。

Abstract: Pythium spp. were isolated from the rhizosphere soils of corn sampled from Honggu and Qilihe districts of Lanzhou using a baiting method. Twenty three isolates were obtained after subculture and purification. Based on the morphology, culture characteristics, growth rate, adaptation to temperatures and sequence analysis of rDNA-ITS, the 23 isolates were all identified as P. scleroteichum, P. ultimum or Pythium sp. This is the first report of P. scleroteichum in China and of P. ultimum from corn in Gansu. P. ultimum was the dominant population of the three species (60.9% of the isolations), while Pythium sp. (26.1%) was second, and, P. scleroteichum (13.0%) was third.

中图分类号:

S435.131

柴兆祥,李金花,楼兵干,李唯,甘辉林,郭成,赵玲,董迪. 玉米根围土壤中腐霉菌的分离鉴定及核糖体DNA-ITS序列分析[J]. 草业学报, 2009, 18(3): 126-135.

CHAI Zhao-xiang, LI Jin-hua, LOU Bing-gan, LI Wei, GAN Hui-lin1,
GUO Cheng, ZHAO Ling, DONG Di. Isolation, identification, and sequence analysis of rDNA-ITS of
Pythium species sampled from rhizosphere soil of corn[J]. Acta Prataculturae Sinica, 2009, 18(3): 126-135.

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