两个高羊茅无性系的营养器官组织培养及再生体系的建立

草业学报 ›› 2009, Vol. 18 ›› Issue (5): 168-175.

两个高羊茅无性系的营养器官组织培养及再生体系的建立

赵智燕,潘俊松,何亚丽^*,王琛,闫军辉

上海交通大学农业与生物学院,上海 200240

收稿日期:2008-12-15 出版日期:2009-10-20 发布日期:2009-10-20
作者简介:赵智燕(1982-), 女,山东胶州人,在读硕士。E-mail:zhaozhiyan009@hotmail.com
基金资助:
上海市农业委员会科技兴农重点攻关项目和国家科技部“十一五”科技支撑计划(2006BAD01A19-4-6)资助。

Tissue culture and plantlet regeneration from vegetative organs of two clones of tall fescue (Festuca arundinacea)

ZHAO Zhi-yan, PAN Jun-song, HE Ya-li, WANG Chen, YAN Jun-hui

Department of Plant Science, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2008-12-15 Online:2009-10-20 Published:2009-10-20

摘要/Abstract

摘要： 以2个高羊茅无性系上农矮生高羊茅(SACD)和98-19的幼穗、叶尖、幼茎和幼节等营养器官作为外植体,在MS培养基上分别添加不同浓度的2,4-二氯苯氧乙酸(2,4-D),探索其对愈伤组织的诱导、继代和分化的最佳浓度,以建立高羊茅无性系的高效再生体系。结果表明,幼穗是诱导愈伤组织的最好的营养器官外植体,出愈率最高达94%,而幼叶尖、幼茎、幼节则未能诱导出愈伤组织;不同浓度2,4-D诱导愈伤组织的出愈率存在显著差异,最佳诱导浓度范围为7～9 mg/L,出愈率可以达83%～94%;2个无性系愈伤诱导率间有显著差异,但与2,4-D浓度之间的互作不显著;继代培养基以MS加入2,4-D 4 mg/L的处理方案最好,胚性愈伤组织出愈率和绿色芽点诱导率最高,分别达到98%和78%,所得的胚性愈伤组织在MS+2,4-D 2 mg/L+6-苄氨基嘌呤(6-BA )1 mg/L分化培养基中的绿苗分化率较高,为57%;生根培养基采用1/2 MS+α-萘乙酸(NAA) 0.5 mg/L,生根率达100%。

Abstract: In an effort to optimize the tissue culture response of a tall fescue (Festuca arundinacea) regeneration system, the effects of 2,4-D on tissue culture responses were investigated using immature inflorescences, leaf tips, young stems, and young nodes as explants. Two clones (SACD and 98-19), which are self-bred dwarf clones of tall fescue were used. Immature inflorescences were the best vegetative organ for callus induction, with the highest callus induction rate of 94%. For tips of leaves, stems and nodes, the callus induction rate was 0%; the optimal concentration of 2,4-D was from 7 to 9 mg/L in MS with a callus induction rate of 83% to 94%. Significant differences were also observed between the tested clones but the interaction between 2,4-D concentrations and the clones was not significant. Subculture medium of 2,4-D 4 mg/L in MS resulted in the highest embryogenic callus induction rate (98%), highest green spot induction rate (78%) during subculture, and high green plant regeneration rate (57%) in regeneration medium of “MS+2,4-D 2 mg/L+6-BA 1 mg/L”. The rooting medium used (“1/2 MS+NAA 0.5 mg/L”), gave a rooting rate of 100%.

中图分类号:

赵智燕,潘俊松,何亚丽,王琛,闫军辉. 两个高羊茅无性系的营养器官组织培养及再生体系的建立[J]. 草业学报, 2009, 18(5): 168-175.

ZHAO Zhi-yan, PAN Jun-song, HE Ya-li, WANG Chen, YAN Jun-hui. Tissue culture and plantlet regeneration from vegetative organs of two clones of tall fescue (Festuca arundinacea)[J]. Acta Prataculturae Sinica, 2009, 18(5): 168-175.

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