镉胁迫下蚕豆幼苗抗氧化能力对外源NO和H2O2的响应

摘要/Abstract

摘要： 以蚕豆品种临蚕2号为试验材料,采用土培法,研究了在镉胁迫期间蚕豆幼苗抗氧化酶(SOD、CAT和POD)活性、脯氨酸、丙二醛和活性氧含量的动态变化过程,以探讨外源NO和H₂O₂对镉毒害下蚕豆幼苗的保护效应。结果表明,0.1 mmol/L SNP(外源NO供体)和0.01 mmol/L H₂O₂单独处理均可缓解0.1 mmol/L镉对蚕豆幼苗的氧化损伤,而二者互作处理存在正协同效应,主要表现在增强了蚕豆幼苗的保护酶活性,其中SOD在镉胁迫72 h后活性达到峰值,比对照提高57.89%,CAT和POD均在镉胁迫48 h后活性达到最大值,分别比对照提高69.60%和101.18%;镉胁迫120 h时,与单镉胁迫处理相比,互作处理的脯氨酸含量提高了134.88%,而MDA含量、内源H₂O₂含量和体内O₂^·-的产生速率下降,有效地增强了蚕豆幼苗在镉胁迫期间的抗氧化能力。

Abstract: This study investigated the change of antioxidant enzymes (including SOD, CAT and POD), proline content, MDA and reactive oxygen species (ROS) in horsebean seedlings under Cd stress, and to study the protective effect of NO and H₂O₂. Sodium nitro presside (SNP) was used as a NO donor. Application of SNP and H₂O₂, alone and in mixtures helped protect horsebean seedling leaves from Cd oxidative damage. However, treatment E showed a much better protective impact than treatments C and D, which significantly increased activities of antioxidant enzymes. Seventy two hours after Cd stress, SOD reached its maximum (57.89% over the control). Forty eight hours after Cd stress, the activities of CAT and POD reached a maximum (69.60% and 101.18% over the control). One hundred and twenty hours after Cd stress, the proline content in treatment E increased by 134.88% compared with treatment B, and the contents of MDA, and H₂O₂ were largely reduced as was the production rate of O₂^·-.

中图分类号:

李源,李金娟,魏小红^*. 镉胁迫下蚕豆幼苗抗氧化能力对外源NO和H₂O₂的响应[J]. 草业学报, 2009, 18(6): 186-191.

LI Yuan,LI Jin-juan,WEI Xiao-hong. Responses of antioxidative capability in horsebean seedling to NO and H₂O₂ under Cd stress[J]. Acta Prataculturae Sinica, 2009, 18(6): 186-191.

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镉胁迫下蚕豆幼苗抗氧化能力对外源NO和H₂O₂的响应

Responses of antioxidative capability in horsebean seedling to NO and H₂O₂ under Cd stress

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