保护性耕作对菘蓝光合特性和保护酶活性的影响

草业学报 ›› 2010, Vol. 19 ›› Issue (1): 113-120.

保护性耕作对菘蓝光合特性和保护酶活性的影响

杨江山¹,张恩和^1*,黄高宝¹,张仁陟²

1.甘肃农业大学农学院,甘肃兰州 730070;
2.甘肃农业大学资源与环境学院,甘肃兰州 730070

收稿日期:2009-07-21 出版日期:2010-01-25 发布日期:2010-02-20
作者简介:杨江山(1969-),男,甘肃古浪人,副教授,在读博士。E-mail:yangjs@gsau.edu.cn
基金资助:
国家科技支撑计划项目(2006BAD15B06)资助。

Effects of conservation tillage on photosynthetic characteristics andprotective enzyme activity of Isatis indigotica

YANG Jiang-shan¹, ZHANG En-he¹, HUANG Gao-bao¹, ZHANG Ren-zhi²

1.Agronomy College of Gansu Agricultural University, Lanzhou 730070, China;
2.Resource
and Environment Faculty of Gansu Agricultural University, Lanzhou 730070, China

Received:2009-07-21 Online:2010-01-25 Published:2010-02-20

摘要/Abstract

摘要： 以T为对照,研究了干旱地区不同生育时期NT和NTS对菘蓝叶片光合特性、WUE、叶绿素含量和保护酶活性的影响。试验结果表明,6、8和10月菘蓝Pn日变化为典型的午休双峰曲线,日均和年均Pn均为NTS＞NT＞T。Gs早上出现高峰,中午和下午降至低水平小幅波动,日均和年均Gs都为NTS＞NT＞T。WUE变化趋势同Gs相似,与Pn日变化无相关性,6和8月菘蓝叶片WUE日均值NTS＞NT＞T,NTS处理效果显著,10月WUE日均值T＞NT＞NTS,WUE年均值NTS＞NT＞T,说明生育后期适度干旱提高菘蓝WUE作用较好。Ci与Pn日变化表现出明显的负相关性,说明菘蓝Pn午休为气孔限制所致。不同生育时期Tr日变化也为午休双峰曲线,与Pn日变化表现出明显的正相关性,日均和年均Tr值均为NTS＞NT＞T。Chla、Chlb、Chl(a+b)和Chla/Chlb年均值均为NTS＞NT＞T,说明NTS和NT有利于Chla和Chlb形成,NTS尤其延缓了生育后期Chla降解,从而提高了Pn。保护性耕作在6和10月干旱季节显著提高了CAT活性,8月高温季节显著提高了SOD活性,SOD、POD和CAT保护酶协同作用,显著降低了膜脂过氧化伤害,MDA年均值T＞NT＞NTS,说明NTS和NT保护酶活性的增强为菘蓝正常生长和Pn提高创造了良好的生理基础,NTS作用最显著。

Abstract: Effects of different tillage practices, i.e. T, NT and NTS, on photosynthetic characteristics, WUE, chlorophyll content and protective enzyme activity of Isatis indigotica were studied in different months. Diurnal change of Pn was a typical bimodal curve in different months, the diurnal average and annual average of Pn displayed NTS＞NT＞T. Gs had a flood tide in the morning,then falled to a low level fluctuation. The diurnal average and annual average of Gs displayed NTS＞NT＞T too.WUE diurnal change was similar to Gs, had no correlation to Pn. WUE diurnal average were NTS＞NT＞T in June and August,but T＞NT＞NTS in October,and WUE annual average were NTS＞NT＞T.It indicated that conservation tillage could storage water in soil,especially NTS.And in growing later season, moderate drought was propitious to improve WUE.Ci and Pn diurnal variation showed a marked negative correlation, it indicated that Pn lunch break due to stomatal limitation. Tr diurnal change was a typical bimodal curve in different months,and showed significant positive correlation with Pn. The diurnal average and annual average of Tr were NTS＞NT＞T also.Conservation tillage improved Chla and Chl(a+b) content significantly, and all the annual average of Chla, Chlb, Chl(a+b) and Chla/Chlb were NTS>NT>T. NT and NTS delayed Chla degradation,then enhanced Pn, NTS had the best effect of all. Conservation tillage improved CAT activity markedly in dry months of June and October,and improved SOD activity of NTS markedly in higher temperature.Because of SOD, POD and CAT coordinated protection,cellmembrane lipid peroxidation injury was reduced significantly.MDA annual average was T>NT>NTS.It illustrated that protective enzyme activity enhancing under NTS and NT,especially NTS,made a good physiological basis to Pn boost and normal growth of I. indigotica.

中图分类号:

S344
Q945.11

杨江山,张恩和,黄高宝,张仁陟². 保护性耕作对菘蓝光合特性和保护酶活性的影响[J]. 草业学报, 2010, 19(1): 113-120.

YANG Jiang-shan, ZHANG En-he, HUANG Gao-bao, ZHANG Ren-zhi. Effects of conservation tillage on photosynthetic characteristics andprotective enzyme activity of Isatis indigotica[J]. Acta Prataculturae Sinica, 2010, 19(1): 113-120.

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