棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建

草业学报 ›› 2010, Vol. 19 ›› Issue (3): 170-176.

棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建

孟亚雄^1,2,张金文³,马小乐³,张金林⁴,仲军³,王化俊^2*

1.甘肃农业大学研究测试中心, 甘肃兰州 730070;
2.甘肃省作物遗传改良与创新重点实验室, 甘肃兰州 730070;

3.甘肃农业大学农学院,甘肃兰州 730070;
4.兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2009-11-20 出版日期:2010-03-25 发布日期:2010-06-20
作者简介:孟亚雄(1977-),男,甘肃会宁人,助理研究员,在读博士。E-mail:yxmeng@163.com
基金资助:
甘肃省农业生物技术研究与应用开发项目(GNSW-2007-01)资助

Constructing expression vector of genes phaB and phaC drived by specific promoter LTP12 for cotton fiber

MENG Ya-xiong^1,2, ZHANG Jin-wen³, MA Xiao-le³, ZHANG Jin-lin⁴, ZHONG Jun³, WANG Hua-jun²

1.Instrumental Research and Analysis Center, Gansu Agricultural University, Lanzhou 730070, China;

2.Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China;

3.College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
4.College of
Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2009-11-20 Online:2010-03-25 Published:2010-06-20

摘要/Abstract

摘要： 聚羟基丁酸酯(PHB)具有抗皱、保暖等特性,若棉纤维中存在PHB则棉纤维保暖性和抗皱性将会提高,因此通过基因工程技术手段将聚羟基丁酸酯的合成酶基因导入棉花中,就有可能在棉纤维发育过程中合成PHB,提高棉纤维的抗皱性、保暖性和防水等特性,从而达到改良棉纤维的品质。本研究分离了棉纤维特异启动子LTP12和聚羟基丁酸酯合成酶基因phaB和phaC,并构建了由LTP12驱动的基因phaB和phaC的双价植物表达载体pBLTPPBPC。

Abstract: The poly-3-hydroxybutyrate(PHB) possesses anti-wrinkle and stay-warm characteristics. It has been hypothesized that the anti-wrinkle and stay-warm ability of cotton fiber would be improved if fiber contains PHB. The genetic engineered cotton plant containing the PHB synthesis would theoretically increase the incorporation of PHB into cotton fiber. As a result, the waterproof ability, anti-wrinkle and stay-warm characteristics of cotton fiber should be improved. In this study, the cotton fiber specific promoter LTP12 and two PHB synthesis genes (phaB and phaC) were integrated into a pBI121. Therefore, a new plant expression vector pBLTPPBPC was constructed.

中图分类号:

Q943.2

孟亚雄,张金文,马小乐,张金林,仲军,王化俊. 棉纤维特异启动子LTP12驱动的基因phaB、phaC双价载体构建[J]. 草业学报, 2010, 19(3): 170-176.

MENG Ya-xiong, ZHANG Jin-wen, MA Xiao-le, ZHANG Jin-lin, ZHONG Jun, WANG Hua-jun. Constructing expression vector of genes phaB and phaC drived by specific promoter LTP12 for cotton fiber[J]. Acta Prataculturae Sinica, 2010, 19(3): 170-176.

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