Abstract: The effect of compounding proportions of different hormones on explants (cotyledon, stem, eustipes, and hypocotyl) were studied in tissue cultures of the succulent xerophyte Zygophyllum xanthoxylum, and a stable, high-efficiency plant regeneration system was established. Cotyledons were the best type of explant for callus induction and eustipes were the best for adventitious bud induction. The best callus inducing medium for Z. xanthoxylum was MS containing 0.1 mg/L 6-BA and 1.0 mg/L NAA, in which the callus induction rate for cotyledons was 100% with an induction time of 20 d, shorter than the previously reported 30 d. The best medium to induce adventitious buds was MS containing 1.5 mg/L 6-BA and 0.5 mg/L NAA, in which shoot differentiation of eustipes reached 82.5% and the induction time wais 7-weeks, shorter than the earlier report of 10-weeks. The best medium for rooting was 1/2 MS containing 1.0 mg/L NAA and 0.5 mg/L IBA, in which the frequency of rooting reached 86.7%, 13.4% greater than the previous result of 73.3%. In this medium, adventitious roots began to appear in 13 d (earlier than the reported 20 d) and the transition from callus to regeneration plant was completed in (84±4) d.