鱼腥草不同地理群体遗传结构与变异的研究

草业学报 ›› 2011, Vol. 20 ›› Issue (2): 227-233.

鱼腥草不同地理群体遗传结构与变异的研究

钟军^1,3,王坤²,仇萍³,曾维军³,熊兴耀^4*

1.湖南农业大学农学院,湖南长沙 410128;
2.湖南农业大学生命科学与技术学院,湖南长沙 410128;
3.湖南正清制药集团股份有限公司,湖南怀化 418000;
4.湖南农业大学园林园艺学院,湖南长沙 410128

收稿日期:2010-03-10 出版日期:2011-02-25 发布日期:2011-04-20
通讯作者: E-mail:xiongxingyao@yahoo.cn
作者简介:钟军(1973-),女,湖南沅江人,副教授,博士。
基金资助:
湖南省科技计划基金(2010KJ008),湖南省教育厅基金(2010JY111)和湖南农业大学人才引进基金(2003YJ007)资助。

Genetic structure and variation in different geographical populations of Houttuynia cordata

ZHONG Jun^1,3, WANG Kun², QIU Ping³, ZENG Wei-jun³, Xiong Xing-yao⁴

1.College of Agriculture, Hunan Agricultural University, Changsha 410128, China;
2.College of Bioscience and technology, Hunan Agricultural University, Changsha 410128, China;
3.Hunan Zhengqing Pharmaceutical Co.LTD,Huaihua 418000, China;
4.College of Horticulture and Gardening, Hunan Agricultural University, Changsha 410128, China

Received:2010-03-10 Online:2011-02-25 Published:2011-04-20

摘要/Abstract

摘要： 采用SRAP分子标记技术对湖南省怀化地区16个野生鱼腥草群体的遗传特征进行了分析。结果表明,16个鱼腥草群体的多态位点比例(PPB)为53.66%~85.35%,Nei基因多样性指数(h)为0.07~0.23,Shannon表型信息指数(I)为0.10~0.34,说明在鱼腥草群体水平上有相对较高的遗传变异;群体间的基因分化系数(Gst)为0.51,表明大部分的遗传变异均存在于群体间,同时群体内具有一定频率的基因流(Nm=0.326)。UPGMA聚类分析表明,16个群体在遗传距离为0.5处分为五大类群,并且有一定的地缘关系。相关性分析表明,虽然16个鱼腥草群体的遗传结构与生境因子相关均不显著,但基因分化系数(Gst)和基因流(Nm)随海拔的增加有上升而随着纬度和经度的增加有下降的趋势。

Abstract: The genetic diversity and genetic structure of Houttuynia cordata populations in Huaihua were analyzed by SRAP (sequence-related amplified polymorphism). The percentages of polymorphy(PPB)of the sixteen populations varied from 53.66% to 85.35% and the average heterozygosities ranged from 0.07 to 0.23,The Shannon index of the total population was 0.10-0.34, which suggested relatively high genetic variability in H. cordata. The genetic differentiation coefficient (Gst) was 0.51 so it is proposed that most of the genetic variation occurred between populations with a certain frequency of gene flow within populations(Nm=0.326). A cluster analysis based on the UPGMA method grouped the majority of the 16 populations at D=0.5 into five main clusters that corresponded to their geographic regions. The results suggested there was a lack of significant association between genetic diversity and environmental factors among the 16 H. cordata populations, although Gst and Nm were enhanced at higher altitudes, where were declining along with raising latitude and longitude.

中图分类号:

钟军,王坤,仇萍,曾维军,熊兴耀. 鱼腥草不同地理群体遗传结构与变异的研究[J]. 草业学报, 2011, 20(2): 227-233.

ZHONG Jun, WANG Kun, QIU Ping, ZENG Wei-jun, Xiong Xing-yao. Genetic structure and variation in different geographical populations of Houttuynia cordata[J]. Acta Prataculturae Sinica, 2011, 20(2): 227-233.

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