不同防褐化措施对苏丹草愈伤诱导以及抗褐化的效果研究

草业学报 ›› 2011, Vol. 20 ›› Issue (3): 174-181.

不同防褐化措施对苏丹草愈伤诱导以及抗褐化的效果研究

吕宗友,苏衍菁,赵国琦^*,邬彩霞

扬州大学动物科学与技术学院,江苏扬州 225009

收稿日期:2010-04-12 出版日期:2011-03-25 发布日期:2011-06-20
通讯作者: E-mail: jszhaoguoqi@sohu.com
作者简介:吕宗友(1983-),男,四川德阳人,硕士。E-mail:2zongyou@163.com
基金资助:
江苏省研究生科技创新基金资助。

Effects of anti-browning agents on the induction of sudangrass callus

LV Zong-you, SU Yan-jing, ZHAO Guo-qi, WU Cai-xia

Institute of Animal Science and Technology of Yangzhou University, Yangzhou 225009, China

Received:2010-04-12 Online:2011-03-25 Published:2011-06-20

摘要/Abstract

摘要： 苏丹草组织材料在组织培养过程中会产生酚类物质,影响愈伤组织的生长,甚至导致愈伤组织死亡。本试验以苏丹草幼穗为外植体,探讨了不同防褐化措施对苏丹草愈伤诱导的影响及抗褐化的效果防止。结果表明,活性炭、V_C、PVP和AgNO₃均能不同程度地提高愈伤诱导率和降低褐化率。其中,培养基中低浓度的蔗糖(10~20 g/L)能降低褐化率,但同时也降低了愈伤诱导率,而高浓度的蔗糖(40~50 g/L)能提高愈伤诱导率但同时也增加了褐化率。柠檬酸能提高愈伤诱导率但不能防止褐化。当活性炭的添加量为0.15 g/L时,愈伤的诱导及褐化防止效果最好。

Abstract: Sudangrass can easily produce phenolic compounds when it is grown in tissue culture. The browning phenomenon is harmful to callus growth, leading to callus death. Anti-browning agents were used to study browning. Active carbon(AC), vitamin C(V_C), polyvinyl pyrrolidone(PVP), and AgNO₃ all improved the number of inductions and reduced the numbers browning. Low concentrations of sucrose (10-20 g/L) reduced the numbers browning but did not improve the induction number, while high concentrations increased both. Citric acid improved only the induction numbers and did not affect the numbers browning. When the concentration of AC was 0.15 g/L, the callus was best both in terms of induction number and anti-browning.

中图分类号:

吕宗友,苏衍菁,赵国琦,邬彩霞. 不同防褐化措施对苏丹草愈伤诱导以及抗褐化的效果研究[J]. 草业学报, 2011, 20(3): 174-181.

LV Zong-you, SU Yan-jing, ZHAO Guo-qi, WU Cai-xia. Effects of anti-browning agents on the induction of sudangrass callus[J]. Acta Prataculturae Sinica, 2011, 20(3): 174-181.

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