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草业学报 ›› 2012, Vol. 21 ›› Issue (1): 220-228.

• 研究论文 • 上一篇    下一篇

一年生黑麦草制取燃料酒精发酵工艺的初步研究

代王莹,郭和蓉*,张兴龙,李强   

  1. 华南农业大学农学院,广东 广州 510642
  • 出版日期:2012-02-20 发布日期:2012-02-20
  • 通讯作者: E-mail:guoherong@scau.edu.cn
  • 作者简介:代王莹(1986-),女,江西樟树人,在读硕士。
  • 基金资助:
    广东省科技计划项目(工业攻关计划,能源技术)(2006A10703003)和广东生物质产业化发展研究(广东软科学计划项目)(2007B070900111)资助。

A preliminary study on fermentation technology in the manufacture of fuel alcohol using annual ryegrass

DAI Ying, GUO He-rong, ZHANG Xing-long, LI Qiang   

  1. Agricultural Science College, South China Agricultural University, Guangzhou 510642, China
  • Online:2012-02-20 Published:2012-02-20

摘要: 以分别经过酸预处理(1% H2SO4)和碱预处理(10% NaOH),再用绿色木酶酶解的一年生黑麦草邦德(Abundant)为材料,进行了燃料酒精发酵工艺研究。结果表明,通过预处理后,方案1(普通酵母+毕赤酵母,体积比7∶3)的相对最优发酵条件为:温度37℃,菌株浓度10%,pH 5.5,时间48 h。方案2(普通酵母+毕赤酵母+黑曲霉, 体积比7∶3∶2)的相对最优发酵条件为:温度37℃,菌株浓度12%,pH 5.5,时间48 h。2种方案中,菌株浓度和发酵温度是比较重要的影响因素。对于酸碱预处理而言,均是发酵方案2的原料酒精转化率相对较高,分别为8.36%和8.39%,差异不显著。

Abstract: The fermentation technology of fuel alcohol manufacturing transformation was studied using an annual ryegrass (Lolium multiflorum) variety for abundant material. It was pretreated with 1% H2SO4 and 10% NaOH then hydrolyzed by Trichoderma viride. The relative optimum conditions of program 1 (a volume ratio of Saccharomyces cerevisiae to Pichia pastoris of 7∶3) were a 10% concentration of the yeasts at 37℃, pH 5.5 for 48 h. For program 2 (a ratio of S. cerevisiae, P. pastoris, and Aspergullus niger of 7∶3∶2) a 12% concentration of the mixture at 37℃, pH 5.5 was tested for 48 h. The concentration of strains and the fermentation temperature were the more important factors in both programs. For pretreatments with H2SO4 and NaOH, program 2 achieved relatively higher alcohol conversion rates (8.36% and 8.39%, respectively) but there was no significant difference.

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