早春短命植物绵果荠愈伤组织诱导相关因素研究

草业学报 ›› 2012, Vol. 21 ›› Issue (2): 116-122.

早春短命植物绵果荠愈伤组织诱导相关因素研究

曼苏尔·那斯尔,谭敦炎,廖康^*

新疆农业大学,新疆乌鲁木齐 830052

收稿日期:2011-01-13 出版日期:2012-02-25 发布日期:2012-04-20
通讯作者: E-mail:liaokang01@163.com
作者简介:曼苏尔·那斯尔(1983-),男,维吾尔族,新疆阿克苏人,在读硕士。E-mail:mansur813@163.com
基金资助:
新疆维吾尔自治区高技术研究与发展计划项目(200810102)资助。

The relevant factors of callus induction of early spring ephemeral Lachnoloma lehmannii (Brassicaceae)

MANSUR Nasir, TAN Dun-yan, LIAO Kang

Xinjiang Agricultural University, rümqi 830052, China

Received:2011-01-13 Online:2012-02-25 Published:2012-04-20

摘要/Abstract

摘要： 绵果荠是分布在新疆北部准噶尔荒漠中的十字花科一年生早春短命植物。本研究为探讨绵果荠愈伤组织诱导条件,以无菌苗子叶为外植体,研究了不同苗龄、培养基种类、蔗糖浓度和培养条件对愈伤组织的诱导及其生长的影响。结果表明,1)愈伤组织诱导的最佳条件为:苗龄14 d、培养基为MS+1.0 mg/L IBA+0.1 mg/L BAP、含糖量3%、温度25℃和光照为黑暗。2)愈伤组织生长的适宜条件是光照16 h/d和温度25℃。

Abstract: Lachnoloma lehmannii is an annual early spring ephemeral Brassicaceae species in the Junggar Desert of northern Xinjiang. The primary aims of this study were to research the conditions for callus induction in L. lehmannii. Using the aspect seedling cotyledon as explants, the effect of seedling age, media, sucrose concentration and the culture condition on callus induction and their growth was investigated. The most suitable condition for callus induction was seedlings of optimal age (14 days) on an MS culture medium containing 1.0 mg/L IBA+0.1 mg/L BAP, with a suitable concentration of sucrose (3%), at a temperature of 25℃ in the dark. The best condition for callus growth was a temperature of 25℃ in the light with a photoperiod of 16 h/day.

中图分类号:

Q944.6

曼苏尔·那斯尔,谭敦炎,廖康. 早春短命植物绵果荠愈伤组织诱导相关因素研究[J]. 草业学报, 2012, 21(2): 116-122.

MANSUR Nasir, TAN Dun-yan, LIAO Kang. The relevant factors of callus induction of early spring ephemeral Lachnoloma lehmannii (Brassicaceae)[J]. Acta Prataculturae Sinica, 2012, 21(2): 116-122.

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