日粮与牛组织中稳定性碳同位素的相关性研究

草业学报 ›› 2012, Vol. 21 ›› Issue (2): 205-211.

日粮与牛组织中稳定性碳同位素的相关性研究

孙丰梅^1,2,王慧文³,石光雨¹,杨曙明^2*

1.河北北方学院食品科学系,河北张家口075131;
2.中国农业科学院农业质量标准与检测技术研究所,北京100081;
3.河北北方学院牧业工程系,河北张家口075131

收稿日期:2011-01-10 出版日期:2012-02-25 发布日期:2012-04-20
通讯作者: E-mail:yangshumingcaas@sina.com
作者简介:孙丰梅(1977-),女,河北新乐人,副教授,博士。E-mail:sunfengmei1977@yahoo.com.cn
基金资助:
国际科技合作项目“牛产品溯源检测技术研究”(2006DFA32170)资助。

Relationship of stable carbon isotope concentration in diet and tissues of beef cattle

SUN Feng-mei^{1, 2}, WANG Hui-wen³, SHI Guang-yu¹, YANG Shu-ming²

1.Department of Food Science, Hebei North University, Zhangjiakou 075131, China;
2.Institute of Quality Standard and Testing Technology for Agro-Product, Chinese Academy of Agricultural Science, Beijing 100081, China;
3.Department of Animal Husbandry and Engineering, Hebei North University, Zhangjiakou 075131, China

Received:2011-01-10 Online:2012-02-25 Published:2012-04-20

摘要/Abstract

摘要： 研究了日粮中C₃和C₄饲料组成不同时,牛不同组织中δ¹³C值的变化规律。18头12~14月龄的青年牛随机分为6组,分别饲喂C₄植物含量为52.0%,61.6%,71.2%,80.8%,90.4%,100.0%的饲料,其他日粮成分为C₃植物,132 d后屠宰。利用同位素比值质谱仪测定了牛尾毛、脱脂牛肉、粗脂肪、各种饲料的δ¹³C值。结果表明,牛组织中碳同位素组成主要受饲料的影响,随着时间的延长,饲喂同一种饲料的牛个体间碳同位素组成差异减小;牛尾毛、脱脂肌肉、粗脂肪中的δ¹³C值随着C₄植物含量在牛饲料中的比例增大而升高,并且均与C₄植物含量呈极显著的相关性(P＜0.01);牛尾毛、脱脂肌肉、粗脂肪中的δ¹³C值依次递减,三者之间相关性达到极显著水平(P＜0.01)。用牛组织中的δ¹³C值可以预测日粮中C₄植物所占的比例,牛尾毛、脱脂肌肉、粗脂肪均可作为牛肉溯源的材料。

Abstract: The effect of quantity of C₃ or C₄ plants in diets on the carbon (δ¹³C) stable isotope composition of different types of beef tissues were investigated. Eighteen (12 to 14 months old) young bulls were randomly divided into six groups and fed with a diet consisting of 52.0%,61.6%,71.2%,80.8%,90.4%,or 100% C₄ plant material. The remainder of the dietary constituents was from C₃ plants. The cattle were butchered after 132 days. The δ¹³C values of cattle tail hair, defatted muscle, crude fat and each feed material were determined by isotope ratio mass spectrometry (IRMS). The stable carbon isotope compositions of the beef tissues were primarily affected by diet, and the difference of δ¹³C value of cattle tail hair fed on the same diet decreased with time. The δ¹³C values of cattle tail hair, defatted muscle and crude fat all become enriched with increasing proportions of C₄ constituents in the diet. Moreover, all δ¹³C values of the different tissues (cattle tail hair＞defatted muscle＞crude fat) were significantly correlated with the content of C₄ plant material. Based on these results, it is possible for the proportion of C₄ plant material to be estimated from the δ¹³C values of the different tissue samples, thus the cattle tail hair, defatted muscle and crude fat can all be used to trace back to diet.

中图分类号:

S823.4

孙丰梅,王慧文,石光雨,杨曙明. 日粮与牛组织中稳定性碳同位素的相关性研究[J]. 草业学报, 2012, 21(2): 205-211.

SUN Feng-mei, WANG Hui-wen, SHI Guang-yu, YANG Shu-ming. Relationship of stable carbon isotope concentration in diet and tissues of beef cattle[J]. Acta Prataculturae Sinica, 2012, 21(2): 205-211.

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