水淹对双穗雀稗抗氧化酶活性及碳水化合物含量的影响

草业学报 ›› 2013, Vol. 22 ›› Issue (1): 217-224.

水淹对双穗雀稗抗氧化酶活性及碳水化合物含量的影响

谭淑端^1,2,朱明勇¹,张克荣¹,朱佳文²,魏杲霞²,张全发^1*

1.中国科学院武汉植物园水生植物与流域生态重点实验室,湖北武汉 430074;
2.湖南农业大学生物科学技术学院,湖南长沙 410128

收稿日期:2011-12-22 出版日期:2013-01-25 发布日期:2013-02-20
通讯作者: E-mail:qzhang@wbgcas.cn
作者简介:谭淑端(1979-),女,湖南衡东人,博士。E-mail:tanshuduan@163.com
基金资助:
中国科学院水生植物与流域生态重点实验室开放课题(Y052631S03),湖南省教育厅科学研究项目(10C806),湖南省科技计划一般项目(2011NK3063)和湖南农业大学青年科学基金项目(09QN13)资助。

Effects of submergence on the antioxidative enzymes and carbohydrate contents of Paspalum distichum

TAN Shu-duan^1,2, ZHU Ming-yong¹, ZHANG Ke-rong¹, ZHU Jia-wen², WEI Gao-xia², ZHANG Quan-fa¹

1.Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;
2.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China

Received:2011-12-22 Online:2013-01-25 Published:2013-02-20

摘要/Abstract

摘要： 植物在水淹条件下其体内氧气和二氧化碳等气体扩散受到限制,但其体内的抗氧化酶防御系统为植株抵御厌氧或缺氧胁迫起着重要作用。本试验比较研究了双穗雀稗经受不同程度的水淹胁迫后,植株体内抗氧化酶活性及可溶性糖和淀粉含量的变化。结果表明,1)从抗氧化酶的保护机制来看,水淹处理植株CAT,SOD, POD,GR和APX活性都随着水淹深度和水淹持续时间的增加而增加,且其值都较未淹对照植株高。该结果支持在水淹胁迫条件下,植株通过包括涉及CAT,SOD,POD,GR和APX等多种抗氧化酶共同作用来保护植株免受活性氧分子的毒害。2)被淹双穗雀稗植株茎和根的可溶性糖和淀粉含量随着水淹持续时间的递增而递减。水淹深度越大的处理,随水淹持续时间的递增其下降幅度也相应增大。但从可溶性糖和淀粉含量的变化趋势可以初步说明,水淹双穗雀稗植株体内的淀粉能很好的水解成植株厌氧代谢所需的可溶性糖形式,产生水淹胁迫下维持植株生长或生存所需的能量,从而捍卫其耐水淹能力。

Abstract: Submergence is one of the major abiotic stresses, primarily through restriction in the diffusion of oxygen and carbon dioxide in plants. A complex anti-oxidative defensive system plays an important role in defending anoxia or hypoxia stress. In this study, the physiological responses in antioxidative enzymes and carbohydrate contents of Paspalum distichum to different submergence times were investigated. 1) The activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR) in roots increased with increased submergence duration and depth, implying an integrated pathway involving CAT, SOD, POD, GR, and APX for protection against the detrimental effects of activated oxygen species under submergence. 2) Total soluble carbohydrate and starch contents of shoots and roots decreased with increased submergence depth and duration, and the decrease was progressive and proportional to the magnitude of the submergence stress. The soluble carbohydrate of submerged plants necessary for survival can be replenished from starch hydrolyzation and this was critical for the survival of submerged P. distichum.

中图分类号:

谭淑端,朱明勇,张克荣,朱佳文,魏杲霞,张全发. 水淹对双穗雀稗抗氧化酶活性及碳水化合物含量的影响[J]. 草业学报, 2013, 22(1): 217-224.

TAN Shu-duan, ZHU Ming-yong, ZHANG Ke-rong, ZHU Jia-wen, WEI Gao-xia, ZHANG Quan-fa. Effects of submergence on the antioxidative enzymes and carbohydrate contents of Paspalum distichum[J]. Acta Prataculturae Sinica, 2013, 22(1): 217-224.

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