2株盐碱地燕麦根际促生菌的筛选及其促生作用研究

草业学报 ›› 2013, Vol. 22 ›› Issue (2): 132-139.

2株盐碱地燕麦根际促生菌的筛选及其促生作用研究

刘佳莉,方芳,史煦涵,陈红艳,姚琳,郭长虹^*

哈尔滨师范大学生命科学与技术学院分子细胞遗传与遗传育种黑龙江省重点实验室,黑龙江哈尔滨150025

收稿日期:2012-04-24 出版日期:2013-02-25 发布日期:2013-04-20
通讯作者: E-mail:kaku2008@hotmail.com
作者简介:刘佳莉(1986-),女,黑龙江齐齐哈尔人,在读博士。E-mail:jingai7777@163.com
基金资助:
国家自然科学基金(31170479),国家科技支撑计划项目(2011BAD17B04-2-1)和黑龙江省自然科学基金(C201142)资助。

Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil

LIU Jia-li, FANG Fang, SHI Xu-han, CHEN Hong-yan, YAO Lin, GUO Chang-hong

Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China

Received:2012-04-24 Online:2013-02-25 Published:2013-04-20

摘要/Abstract

摘要： 以1-氨基环丙烷-1-羧酸(ACC)为唯一氮源,采用定向富集筛选方法,从盐碱地燕麦的根际土中筛选出2株具有ACC脱氨酶活性的植物促生菌A-2和A-4,经生理生化和16S rDNA鉴定,确定A-2为肠杆菌属,A-4为假单胞菌属。ACC脱氨酶活性分别为(1.89±0.12) μmol α-KA/(mg Pr·h)和(2.63±0.12) μmol α-KA/(mg Pr·h);随着色氨酸(L-Trp)浓度的增加,A-2的IAA合成量相应增加,A-4的合成量几乎没改变;A-2合成嗜铁素的能力高于A-4。用A-2及A-4处理的燕麦株高由12.24 cm增加到13.98和14.07 cm;植株鲜重由34.29 g增加到35.01和35.10 g;植株根长由14.13 cm增加到16.23和17.60 cm;根鲜重由7.79 g增加到8.07和8.19 g。说明用具有ACC脱氨酶活性的菌株A-2和A-4处理可有效地促进燕麦的生长并提高燕麦的盐碱抗性。

Abstract: Two strains of plant growth-promoting rhizobacteria (PGPR) containing ACC deaminase, A-2 and A-4 were isolated from saline-alkali rhizosphere soil of Avena sativa, based upon their ability to utilize 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source. Using morphology, physiological biochemical characteristics and 16S rDNA analysis, strain A-2 was identified as Enterobacter sp. and A-4 was confirmed as Pseudomonas sp.. The ACC deaminase activity of A-2 and A-4 were (1.89±0.12) μmol α-KA/(mg Pr·h) and (2.63±0.12) μmol α-KA/(mg Pr·h), respectively. When the concentration of L-Trp increased, the IAA synthesis of A-2 increased, while that of A-4 did not change significantly. The ability of A-2 to synthesize siderophore was greater than that of A-4. The height of oat plants treated with A-2 and A-4 were from 12.24 cm up to 13.98 and 14.07 cm, the plant fresh weights were from 34.285 g up to 35.012 and 35.102 g, respectively. The length of roots were from 14.13 cm up to 16.23 and 17.60 cm, and root fresh weights were from 7.790 g up to 8.065 and 8.187 g, respectively. These results suggest that strains A-2 and A-4 contain ACC deaminase that can increase resistance in oat to saline-alkali stress.

中图分类号:

Q945.3

刘佳莉,方芳,史煦涵,陈红艳,姚琳,郭长虹. 2株盐碱地燕麦根际促生菌的筛选及其促生作用研究[J]. 草业学报, 2013, 22(2): 132-139.

LIU Jia-li, FANG Fang, SHI Xu-han, CHEN Hong-yan, YAO Lin, GUO Chang-hong. Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil[J]. Acta Prataculturae Sinica, 2013, 22(2): 132-139.

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