甜高粱根际溶磷菌溶磷能力及菌株特性研究

doi:10.11686/cyxb20140633

草业学报 ›› 2014, Vol. 23 ›› Issue (6): 274-278.DOI: 10.11686/cyxb20140633

甜高粱根际溶磷菌溶磷能力及菌株特性研究

雷学军^1,2,吴毅¹,黄馨¹,蔡建波^1,2,李卓²,吴林世¹

1.中南林业科技大学碳循环研究中心,湖南长沙 410004;
2.湖南绿心农林科技有限公司,湖南长沙 410117

收稿日期:2014-04-17 出版日期:2014-12-20 发布日期:2014-12-20
作者简介:雷学军(1958-),男,湖南安乡人,教授
基金资助:
湖南省科技厅重点项目(S2014S2032)和长沙市科技局重点项目(k1301061-21)资助

Phosphate dissolving capability and the affect of phosphate-dissolving bacteria in the rhizosphere on Sorghum bicolor

LEI Xue-jun^1,2,WU Yi¹,HUANG Xin¹,CAI Jian-bo^1,2,LI Zhuo²,WU Lin-shi¹

1.Research Center of Carbon Cycle, Central South University of Forestry & Technology, Changsha 410004, China;
2.Hunan Lvxin Agriculture and Forestry Science &Technology Co. Ltd., Changsha 410117, China

Received:2014-04-17 Online:2014-12-20 Published:2014-12-20

摘要/Abstract

摘要： 利用蒙金娜培养基,从甜高粱根际分离具有溶磷能力的菌株,通过溶磷圈法筛选出5株溶磷能力较强的菌株,利用钼锑抗比色法和Salkowski比色法分别对溶磷特性与分泌IAA能力进行测定,结果表明,筛选的菌株分解磷酸钙的能力存在显著差异,溶磷量在107.26~233.95 μg/mL之间,各菌株均具有分泌IAA能力,IAA分泌量在15.23~27.79 μg/mL之间,接种溶磷菌后甜高粱株高、茎粗、干重都比对照明显提升。因此, 溶磷能力和分泌IAA能力较强的菌株(WD₅₁、WD₂₀) 可作为研制微生物肥料的优良菌株。

Abstract: Phosphorus is the second of the most necessary elements for plant growth.The phosphorus nutrients needed by plants are mainly sourced from rhizospheric soil, and phosphorus deficiency in the soil is an important limiting factor for agricultural production in many developing countries. Some micro-organisms can convert insoluble phosphate to soluble forms and research on such organisms therefore has considerable practical significance. Five bacterial strains were screened from Mengjinna inorganic mediums. The phosphate-solubilizing characteristics and IAA secretion ability of these strains of bacteria were studied using both the Molybdenum antimony resistance colorimetric method and Salkowski colorimetric method. There were notable differences in the micro-organisms’ phosphate dissolving capability. Phosphate-dissolving capability was 107.26-233.95 μg/mL. All the strains had the ability to produce IAA and IAA capability was 15.23-27.79 μg/mL. In comparison with the control, every strain showed significant benefits to Sorghum bicolor height, stem diameter and dry weight. In summary, strains WD₅₁ and WD₂₀ showed strong capability for phosphate dissolving and IAA secretion and could be used as potential strains for biofertilizer manufacture.

中图分类号:

S514.06

雷学军,吴毅,黄馨,蔡建波,李卓,吴林世. 甜高粱根际溶磷菌溶磷能力及菌株特性研究[J]. 草业学报, 2014, 23(6): 274-278.

LEI Xue-jun,WU Yi,HUANG Xin,CAI Jian-bo,LI Zhuo,WU Lin-shi. Phosphate dissolving capability and the affect of phosphate-dissolving bacteria in the rhizosphere on Sorghum bicolor[J]. Acta Prataculturae Sinica, 2014, 23(6): 274-278.

参考文献

Reference：
[1]Raghothama K G. Phosphate transport and signaling[J]. Current Opinion in Plant Biology, 2000, 3(3):182-187.
[2]Kabznelson H, Peterson E A, Rouatt J W.Phosphate-dissoloving microorganisms on seed and the root zone of plants[J]. Canadian Journal of Botany, 1962, 40(9):1181-1186.
[3]Xie Y P, Li A R, Yan Z L, et al. Effect of different phosphorus levels on phosphorus nutrient uptake, transformation and phosphorus utilization efficiency of oil flax[J]. Acta Prataculturae Sinica, 2014, 23(1):158-166.
[4]Qin F L, Wang J G, Li X L, et al. Effect of VA mycorrhizal fungi and phosphate-solubilizing bacteria on growth and phosphorus uptake of red clover[J]. Acta Prataculturae Sinica, 2000, 9(1):9-14.
[5]Yu W B, Yang X M, Shen Q R, et al. Mechanism on phosphate solubilization of pseudomonas sp. K3 and its phosphate solubilization ability under buffering condition[J].Plant Nutrition and Fertilizer Science, 2010,16(2): 354-361.
[6]Peng Z P, Zhang J T, Yuan S, et al. Effects of different phosphrus application rates on the dynamic accumulation and distribution of dry matter and phosphrus in maize[J]. Plant Nutrition and Fertilizer Science, 2009, 15(4):793-798.
[7]Wu D T, Zhang X X, Gong Z P, et al. Effects of phosphorus nutrition on P absorption and yields of soybean[J].Plant Nutrition and Fertilizer Science, 2012, 18(3): 670-677.
[8]Win M,Nakasathien S,Sarobol E.Effects of phosphorus on seed oil and protein contents and phosphorus use efficiency in some soybean varieties[J].Kasetsart Journal, 2010, 44:1-9.
[9]Li R, Zhou D M, Wu Y, et al. Selection and characteristics of phosphate-solubilizing bacteria in rhizosphere of Cunninghaimia lanceolata[J]. Journal of Central South University of Forestry & Technology, 2012, 32(4):95-99.
[10]Zhao X R, Lin Q M, Li B G.The Solubilization of Four Insoluble Phosphates by Some Microorganisms[J]. Acta Microbiologica Sinica,2002, 42(2): 236-241.
[11]Hariprased P,Niranjana S R.Isolation and characterization of phosphate-dissoloving rhisobacteria to improve plant health of tomato[J]. Plant and Soil, 2009, 316:13-24.
[12]Yao T. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region ⅡPhosphate-solubilizing power and auxin production[J]. Acta Prataculturae Sinica, 2004, 13(3):85-90.
[13]Wang M J. Energy plant-sweet sorghum and ethanol production technology by sweet sorghum stalk[C]. 2005 Shenzhen International Symposium on Renewable Energy Technology and Investment， 2005.
[14]Zhu P M, Yang X M, Xu Y C, et al. High effective phosphate-solubilizing bacteria:Their isolation and promoting effect on corn seedling growth[J].Chinese Journal of Applied Ecology, 2007, 18(1):107-112.
[15]Li Y E, Yao T, Zhu Y, et al. Isolation and characteristics of phosphorus-solubilizing bacteria in rhizosphere of alfalfa and sainfoin in Lanzhou[J]. Chinese Journal of Grassland, 2009, 31(1):45-51.
[16]Lin Q M, Zhao X R. The methods for quantifying capacity of bacteria in dissolving P compounds [J]. Microbiology,2001, 28(1):1-4.
[17] Masoni A,Ercoli L,Mariotti M,et al.Post-anthesis accumulation and remobilization of dry matter,nitrogen and phosphorus in durum wheat as affected by soil type[J].European Journal of Agronomy, 2007, 26: 179-186.
[18] Dordas C.Dry matter,nitrogen and phosphorus accumulation,partitioning and remobilization as affected by N and P fertilization and source-sink relations[J].European Journal of Agronomy,2009, 30:129-139.
[19]Lu R X, Wang X L, Li X G, et al. Capability of dissolving phosphate and characteristics of phosphate-dissolving bacteria in rhizosphere of astragalus bhotanensis in Guizhou[J]. Chinese Journal of Grassland, 2012, 34(4):101-108.
参考文献：
[1]Raghothama K G. Phosphate transport and signaling[J]. Current Opinion in Plant Biology, 2000, 3(3):182-187.
[2]Kabznelson H, Peterson E A, Rouatt J W.Phosphate-dissoloving microorganisms on seed and the root zone of plants[J]. Canadian Journal of Botany, 1962, 40(9):1181-1186.
[3]谢亚萍, 李爱荣, 闫志利, 等. 不同供磷水平对胡麻磷素养分转运分配及其磷肥效率的影响[J]. 草业学报, 2014, 23(1):158-166.
[4]秦芳玲, 王敬国, 李晓林, 等. VA菌根真菌和解磷细菌对红三叶草生长和氮磷营养的影响[J]. 草业学报, 2000, 9(1):9-14.
[5]虞伟斌, 杨兴明, 沈其荣, 等. K3 解磷菌的解磷机理及其对缓冲容量的响应[J]. 植物营养与肥料学报, 2010,16(2): 354-361.
[6]彭正萍, 张家铜, 袁硕, 等. 不同供磷水平对玉米干物质和磷动态积累及分配的影响[J]. 植物营养与肥料学报, 2009, 15(4):793-798.
[7]吴冬婷, 张晓雪, 龚振平, 等. 磷素营养对大豆磷素吸收及产量的影响[J]. 植物营养与肥料学报, 2012, 18(3): 670-677.
[8]Win M,Nakasathien S,Sarobol E.Effects of phosphorus on seed oil and protein contents and phosphorus use efficiency in some soybean varieties[J].Kasetsart Journal, 2010, 44:1-9.
[9]李蓉, 周德明, 吴毅, 等. 杉木根际溶磷菌筛选及其部分特性的初步研究[J]. 中南林业科技大学学报, 2012, 32(4):95-99.
[10]赵小蓉, 林启美, 李保国. 溶磷菌对4种难溶性磷酸盐溶解能力的初步研究[J]. 微生物学报,2002, 42(2): 236-241.
[11]Hariprased P,Niranjana S R.Isolation and characterization of phosphate-dissoloving rhisobacteria to improve plant health of tomato[J]. Plant and Soil, 2009, 316:13-24.
[12]姚拓. 高寒地区燕麦根际联合固氮菌研究Ⅱ固氮菌的溶磷性和分泌植物生长素特性测定[J]. 草业学报, 2004, 13(3):85-90.
[13]王孟杰. 能源作物甜高粱良种培育及其茎秆制取乙醇技术[C]. 2005年度深圳可再生能源技术与投资国际研讨会， 2005.
[14]朱培淼, 杨兴明, 徐阳春, 等. 高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J]. 应用生态学报, 2007, 18(1):107-112.
[15]李玉娥, 姚拓, 朱颖, 等. 兰州地区苜蓿和红豆草根际溶磷菌筛选及菌株部分特性研究[J]. 中国草地学报, 2009, 31(1):45-51.
[16]林启美, 赵小蓉. 细菌解磷能力测定方法的研究[J]. 微生物学通报,2001, 28(1):1-4.
[17] Masoni A,Ercoli L,Mariotti M,et al.Post-anthesis accumulation and remobilization of dry matter,nitrogen and phosphorus in durum wheat as affected by soil type[J].European Journal of Agronomy, 2007, 26: 179-186.
[18] Dordas C.Dry matter,nitrogen and phosphorus accumulation,partitioning and remobilization as affected by N and P fertilization and source-sink relations[J].European Journal of Agronomy,2009, 30:129-139.
[19]陆瑞霞, 王小利, 李显刚, 等. 地八角根际溶磷菌溶磷能力及菌株特性研究[J]. 中国草地学报, 2012, 34(4):101-108.

甜高粱根际溶磷菌溶磷能力及菌株特性研究

Phosphate dissolving capability and the affect of phosphate-dissolving bacteria in the rhizosphere on Sorghum bicolor

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