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草业学报 ›› 2014, Vol. 23 ›› Issue (6): 355-360.DOI: 10.11686/cyxb20140643

• 论文 • 上一篇    

小花碱茅组织培养植株再生体系的建立

王雪芳1,王春梅2,张金林1,段丽婕1,王锁民1,*   

  1. 1.兰州大学草地农业科技学院 草地农业生态系统国家重点实验室,甘肃 兰州 730020;
    2.中国农业科学院兰州畜牧与兽药研究所,甘肃 兰州 730050
  • 收稿日期:2012-12-03 出版日期:2014-12-20 发布日期:2014-12-20
  • 通讯作者: E-mail:smwang@lzu.edu.cn
  • 作者简介:王雪芳(1986-),女,山西忻州人,在读硕士
  • 基金资助:
    国家自然科学基金项目(31170431,31201841)和教育部博士点基金优先发展领域项目(20130211130001)资助

Development of a plant regeneration system via tissue culture in Puccinellia tenuiflora

WANG Xue-fang1,WANG Chun-mei2,ZHANG Jin-lin1,DUAN Li-jie1,WANG Suo-min1   

  1. 1.State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
    2.Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China
  • Received:2012-12-03 Online:2014-12-20 Published:2014-12-20

摘要: 以小花碱茅成熟种子为外植体,研究了脱颖处理和不同激素配比对愈伤组织诱导和分化的影响,成功建立了其组织培养植株再生体系。结果表明,种子脱颖处理显著提高了种子发芽率,降低了污染率;诱导愈伤组织的最佳培养基为添加5.0 mg/L 2,4-D的MS培养基,诱导率可达55.2%,2周可见白色愈伤组织;最佳芽分化培养基为添加1.0 mg/L 6-BA和0.5 mg/L NAA的MS培养基,分化率可达31.7%,同时伴随根毛的发生,生根率达81.7%,诱导时间为2周;分化后的再生苗移植于1/2MS培养基上,100%生根,炼苗移栽后可全部成活。从小花碱茅种子诱导愈伤组织到植株再生共需16周左右。小花碱茅组织培养植株再生体系的建立为进一步探究小花碱茅耐盐碱分子机制奠定了基础。

Abstract: The effects of husk-off treatment and various hormone ratios on callus induction and differentiation were investigated using mature seeds of Puccinellia tenuiflora as explants. Finally, a plant regeneration system via tissue culture was established. The husk-off treatment increased seed germination rate and deceased contamination rate significantly. The optimal medium for callus induction was MS medium supplemented with 5.0 mg/L 2,4-D with the highest callus induction rate of 55.2%. The optimal medium for bud differentiation and rooting was MS medium supplemented with 1.0 mg/L 6-BA+0.5 mg/L NAA with the highest bud differentiation rate of 31.7%, the highest rooting rate of 81.7% within a period of two weeks. The rooting rate of regenerated plants was up to 100% on half strength MS medium and their transplant survival rate in soil was also up to 100%. The whole process of plant regeneration via tissue culture lasted for about 16 weeks totally. The establishment of plant regeneration system via tissue culture in P. tenuiflora has laid a foundation for further exploring the molecular mechanism of its salt tolerance.

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