Abstract: The stoichiometric elements of three dominant species, namely Stipa grandis, Artemisia frigida and Leymus chinensis were measured in different land use (grazing-exclusion, grazing and mowing) in the temperate grassland in Inner Mongolia. The seasonal variations of stoichiometric characteristic of plants and soil under different grassland uses were discussed. The stoichiometric characteristic response mechanisms of the plants and the nutrient limiting conditions were analyzed. The results show that obvious seasonal variations in C, N, P concentrations and their mass ratio of the three species exist in each grassland use type. Grassland use type, species and their interactions have significant impacts on N, P concentrations and the ratios C∶N, C∶P and N∶P, among which C concentration is relatively stable in the whole growing season and different uses show no significant impact on it. N concentration reduces overall. N and P concentrations in leaves are higher in grazing plot than those in grazing-exclusion and mowing plots. It shows that grazing can increased N and P concentrations. On the whole, plant growth is limited by N. Characteristics of soil nutrients among different grassland uses have certain differences, where comparably more serious N deficiency happens in the enclosed plot and P deficiency in the grazing plot. Generally the organic matter, C, N and P concentrations in the grazing plot are higher than those in the mowing and enclosed plots. It shows that soil nutrients can be increased which can promote plants growth in grazing plot.