低温处理下外源水杨酸和脱落酸与内生真菌互作对醉马草共生体的影响

doi:10.11686/cyxb2017106

摘要/Abstract

摘要： 醉马草是我国西北地区的一种天然烈性毒草,其所携带的内生真菌(Epichloё)能促进共生体的生长和提高共生体的生物与非生物抗性。以5 ℃下正常生长一个月的带内生真菌(E⁺)醉马草幼苗和不带内生真菌(E^-)醉马草幼苗为实验材料,测定了外源SA和ABA处理下醉马草幼苗生理生化指标。结果表明,与对照比较,SA和ABA处理增加了叶绿素含量、脯氨酸含量、可溶性糖含量、超氧化物歧化酶活性和过氧化物酶活性,降低了丙二醛含量;SA处理下,在胁迫第4天时,内生真菌显著(P<0.05)提高了脯氨酸含量和超氧化物歧化酶活性,分别提高了51.8%和62.1%。胁迫第6天时,叶绿素含量、丙二醛含量、可溶性糖含量和过氧化物酶活性均达到显著(P<0.05)水平,且SA⁺比SA^-分别显著(P<0.05)提高了23.0%,25.4%,23.7%,18.0%;ABA处理下,内生真菌能显著(P<0.05)提高叶绿素含量、脯氨酸含量、可溶性糖含量、超氧化物歧化酶活性和过氧化物酶活性,但在胁迫第6和8天时,内生真菌却显著(P<0.05)降低了丙二醛含量,ABA⁺比ABA^-分别显著(P<0.05)降低了21.1%和34.3%。表明一定浓度的SA或ABA与内生真菌互作能缓解低温胁迫对醉马草幼苗的伤害。

Abstract: Achnatherum inebrians is an intoxicating grass found in the native grasslands of Northwest China. It forms a symbiotic relationship with Epichloё endophyte which can promote plant growth and improve abiotic and biotic stress resistance. In the present study, endophyte-infected (E⁺) and endophyte-free (E^-) seedlings of A. inebrians were grown at 5 ℃ for one month and exposed to exogenous salicylic acid (SA) and abscisic acid (ABA); the physiological response of seedlings was assessed. The results showed that, comparing with the control, ABA and SA treatments increased the total chlorophyll content, proline acid content, soluble sugar content, superoxide dismutase activity and peroxidase activity but decreased malondialdehyde content. After 4 days of growth under SA stress, Epichloё significantly (P<0.05) increased proline content and superoxide dismutase activity, by 51.8% and 62.1%, respectively. After 6 days of SA stress, the chlorophyll content, malondialdehyde content, soluble sugar content and peroxidase activity were significantly (P<0.05) improved in E⁺ seedlings (23.0%, 25.4%, 23.7% and 18.0% higher than those of E^- seedlings, respectively). Epichloё significantly (P<0.05) increased the chlorophyll content, proline acid content, soluble sugar content, superoxide dismutase activity and peroxidase activity under ABA treatment but significantly (P<0.05) reduced malondialdehyde content after 6 and 8 days of stress; the malondialdehyde content of E⁺ seedlings treated with ABA⁺ were 21.1% and 34.3% lower respectively than E^- seedlings treated with ABA^-. In conclusion, the interactions between ABA and SA and Epichloё endophytes alleviated the damage of low temperature stress on A. inebrians seedlings.

刘静, 陈振江, 李秀璋, 周景乐, 柳莉, 李春杰. 低温处理下外源水杨酸和脱落酸与内生真菌互作对醉马草共生体的影响[J]. 草业学报, 2018, 27(1): 142-151.

LIU Jing, CHEN Zhen-jiang, LI Xiu-zhang, ZHOU Jing-le, LIU Li, LI Chun-jie. Interaction effects of exogenous salicylic acid, abscisic acid and Epichloё on Achnatherum inebrians symbiosis under low temperature stress[J]. Acta Prataculturae Sinica, 2018, 27(1): 142-151.

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