盐胁迫对扁蓿豆幼苗脯氨酸积累及其代谢关键酶活性的影响

doi:10.11686/cyxb2017464

摘要/Abstract

摘要： 以野生扁蓿豆为试验材料,采用盆栽试验方法研究不同盐(NaCl、Na₂CO₃)的不同浓度(0、50、100、200、300、400、500 mmol·L^-1)胁迫对扁蓿豆苗期脯氨酸积累及其代谢途径的关键酶活性的影响。结果表明,在不同盐胁迫下,扁蓿豆脯氨酸(Pro)含量、吡咯啉-5-羧酸合成酶(P5CS)活性、鸟氨酸-δ氨基转移酶(δ-OAT)活性均随盐浓度的升高而逐渐增加,而脯氨酸脱氢酶(ProDH)活性随胁迫浓度的升高而降低。扁蓿豆幼苗主动积累Pro是缓解其NaCl和Na₂CO₃胁迫的原因,并由Glu途径和Orn途径协同作用于Pro积累,但以Orn途径为主。试验中,扁蓿豆所承受的NaCl和Na₂CO₃浓度不宜超过400 mmol·L^-1。

关键词: 扁蓿豆, 盐胁迫, 脯氨酸, 吡咯啉-5-羧酸合成酶, 鸟氨酸-δ氨基转移酶, 脯氨酸脱氢酶

Abstract: In order to study the effects of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism a pot experiment was conducted using wild Medicago ruthenica stressed under different NaCl and Na₂CO₃ solutions; 0, 50, 100, 200, 300, 400 and 500 mmol·L^-1. The result showed that, under salt stress, proline content and P5CS and δ-OAT activity gradually increased while ProDH activity declined with increasing salt concentration. The initial accumulation of proline was a major reason for the reduced damage from NaCl and Na₂CO₃ stress in M. ruthenica seedlings. The Glu and Orn pathways were synergistic to the accumulation of proline, with the Orn pathway playing a leading role. This study suggests that the concentration of NaCl and Na₂CO₃ should not exceed 400 mmol·L^-1 for M. ruthenica.

Key words: Medicago ruthenica, salt stress, proline, pyrroline-5-carboxylate synthetase, ornithine-oxo-acid transaminase, proline dehydrogenase

钟华,董洁,董宽虎. 盐胁迫对扁蓿豆幼苗脯氨酸积累及其代谢关键酶活性的影响[J]. 草业学报, 2018, 27(4): 189-194.

ZHONG Hua, DONG Jie, DONG Kuan-hu. Effect of salt stress on proline accumulation and the activities of the key enzymes involved in proline metabolism in Medicago ruthenica seedlings[J]. Acta Prataculturae Sinica, 2018, 27(4): 189-194.

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