干旱和盐胁迫对盐生植物盐生草种子萌发特性的影响

doi:10.11686/cyxb2020008

草业学报 ›› 2020, Vol. 29 ›› Issue (11): 129-140.DOI: 10.11686/cyxb2020008

干旱和盐胁迫对盐生植物盐生草种子萌发特性的影响

何建军^1,2, 姚立蓉^1,2, 汪军成^1,2, 边秀秀², 司二静^1,2, 杨轲^1,2, 王化俊^1,2, 马小乐^1,2, 李葆春^2,3, 尚勋武¹, 孟亚雄^1,2,*

1.甘肃农业大学农学院,甘肃兰州 730070;
2.甘肃省干旱生境作物学重点实验室,甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070;
3.甘肃农业大学生命科学技术学院,甘肃兰州 730070

收稿日期:2020-01-07 修回日期:2020-02-24 出版日期:2020-11-20 发布日期:2020-11-20
通讯作者: *E-mail: yxmeng1@163.com
作者简介:何建军(1995-),男,甘肃临洮人,在读硕士。E-mail: 3355829261@qq.com

Effects of drought and salt stress on seed germination characteristics of Halogeton glomeratus

HE Jian-jun^1,2, YAO Li-rong^1,2, WANG Jun-cheng^1,2, BIAN Xiu-xiu², SI Er-jing^1,2, YANG Ke^1,2, WANG Hua-jun^1,2, MA Xiao-le^1,2, LI Bao-chun^2,3, SHANG Xun-wu¹, MENG Ya-xiong^1,2,*

1. College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China;
2. Gansu Provincial Key Lab of Aridland Crop Science, Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, China;
3. College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-01-07 Revised:2020-02-24 Online:2020-11-20 Published:2020-11-20
Contact: *E-mail: yxmeng1@163.com
Supported by:
学科建设专项基金(GAU-XKJS-2018-082、083),国家大麦青稞产业技术体系(CARS-05-03B-03),甘肃省重大专项(17ZD2NA016)和盛彤笙创新基金(GSAU-STS-2018-33)资助

摘要/Abstract

摘要： 本研究以盐生植物盐生草为材料,以不同浓度的PEG-6000模拟干旱胁迫和以不同浓度的4种钠盐(NaCl、Na₂SO₄、NaHCO₃、Na₂CO₃)进行盐胁迫,探讨其对盐生草种子萌发特性的影响。结果表明:随着PEG-6000浓度的升高,盐生草种子发芽率、发芽势呈现下降的趋势,幼苗鲜重、干重、株高以及根系活力均呈现先上升后下降的趋势。聚类分析和主成分分析结果显示,以6% PEG-6000为结点,将不同浓度PEG-6000处理聚为两类,鲜重可被首选为评价盐生草萌发期耐干旱的重要参数;随着4种钠盐处理浓度的升高,发芽率、发芽势、幼苗鲜重、干重、株高以及根系活力均呈现不同程度的下降趋势,表明盐胁迫对种子的萌发以及幼苗的生长均有抑制作用,当Na⁺浓度相同时,4种钠盐对盐生草种子萌发及幼苗生长的抑制作用大小为Na₂CO₃>NaHCO₃>NaCl>Na₂SO₄,聚类分析和主成分分析结果显示,以50.00 mmol·L^-1 NaHCO₃,62.50 mmol·L^-1 Na₂SO₄,25.00 mmol·L^-1 Na₂CO₃ 和100.00 mmol·L^-1 NaCl为结点,将各钠盐胁迫浓度聚为两类,其中发芽指标可被首选为评价盐生草萌发期耐NaHCO₃、Na₂SO₄的重要参数,根系活力可被首选为评价盐生草萌发期耐Na₂CO₃的重要参数,干重可被首选为评价盐生草萌发期耐NaCl的重要参数。

关键词: 盐生草, 盐胁迫, 干旱胁迫, 萌发期

Abstract: To explore the effects of drought and salt stress on germination characteristics of halophytes, this study simulated drought stress with different concentrations of PEG-6000 and four sodium salts (NaCl, Na₂SO₄, NaHCO₃, Na₂CO₃) to evaluate the effects of those treatments on the seed germination characteristics of Halogeton glomeratus. It was found the germination percentage and germination energy decreased with increasing PEG-6000 concentration, while the seedling fresh weight, dry weight, plant height and root activity initially increased and then decreased at higher PEG-6000 concentration. Cluster analysis and principal component analysis showed that the different PEG-6000 concentrations could be divided into two groups with 6% PEG-6000 as the point of separation. Fresh weight appeared to be an important parameter. In addition, germination percentage germination energy, seedling fresh weight, dry weight, plant height and root activity declined with increase in concentration of the four sodium salts. This indicated that salt stress inhibited seed germination and seedling growth, with the effects of the four tested sodium salts ranked: Na₂CO₃>NaHCO₃>NaCl>Na₂SO₄. Cluster analysis and principal component analysis showed that the sodium salt concentrations were divided into two groups with the separation points falling at 50.00 mmol·L^-1 NaHCO₃, 62.50 mmol·L^-1 Na₂SO₄, 25.00 mmol·L^-1 Na₂CO₃ and 100.00 mmol·L^-1 NaCl. Among the plant traits evaluated as indicators of H. glomeratus stress tolerance in the germination stage, the germination index was the key indicator of NaHCO₃ and Na₂SO₄ stress, root activity emerged as the primary indicator of Na₂CO₃ stress and dry weight was the major indicator of NaCl stress in H. glomeratus.

Key words: Halogen glomeratus, salt stress, drought stress, germination

何建军, 姚立蓉, 汪军成, 边秀秀, 司二静, 杨轲, 王化俊, 马小乐, 李葆春, 尚勋武, 孟亚雄. 干旱和盐胁迫对盐生植物盐生草种子萌发特性的影响[J]. 草业学报, 2020, 29(11): 129-140.

HE Jian-jun, YAO Li-rong, WANG Jun-cheng, BIAN Xiu-xiu, SI Er-jing, YANG Ke, WANG Hua-jun, MA Xiao-le, LI Bao-chun, SHANG Xun-wu, MENG Ya-xiong. Effects of drought and salt stress on seed germination characteristics of Halogeton glomeratus[J]. Acta Prataculturae Sinica, 2020, 29(11): 129-140.

参考文献

[1] Wang S T, Feng Q, Zhou Y P, et al. Dynamic changes in water and salinity in saline-alkali soils after simulated irrigation and leaching. PLoS One, 2017, 12(11): 25-28.
[2] Wang J C. Study on the salt tolerance mechanisms of ion compartmentation in halophyte Halogeton glomeratus. Lanzhou: Gansu Agricultural University, 2017.
汪军成. 盐生草盐分区隔化耐盐机制研究. 兰州: 甘肃农业大学, 2017.
[3] Chai Y, Sun Z J, Li P Y, et al. Evaluation of the salt resistance of the Cynodon dactylon germplasm from Xinjiang during the seed germination period. Acta Prataculturae Sinica, 2017, 26(8): 154-167.
柴艳, 孙宗玖, 李培英, 等. 新疆狗牙根种质芽期耐盐性综合评价. 草业学报, 2017, 26(8): 154-167.
[4] Chen D B, Wang Q Q, Sun Z Y, et al. Cloning and expression of ZmXTH23 in maize (Zea mays) and its response to salt and drought stress. Journal of Agricultural Biotechnology, 2019, 27(9): 1533-1541.
陈东滨, 王茜茜, 孙智仪, 等. 玉米ZmXTH23的克隆、表达及其对盐胁迫和干旱胁迫的响应. 农业生物技术学报, 2019, 27(9): 1533-1541.
[5] Dong L, Li J Y. Relationship among drought, hydraulic metabolic, carbon starvation and vegetation mortality. Acta Ecologica Sinica, 2013, 33(18): 5477-5483.
董蕾, 李吉跃. 植物干旱胁迫下水分代谢、碳饥饿与死亡机理. 生态学报, 2013, 33(18): 5477-5483.
[6] Wu D Z, Shen Q F, Cai S G, et al. Ionomic responses and correlations between elements and metabolites under salt stress in wild and cultivated barley. Plant & Cell Physiology, 2013, 54(12): 1976-1988.
[7] Sun Z J, Li P Y, Ji P F. Evaluation on the salt tolerance of 38 germplasm resources of Elytrigria repens at seed germination stage. Pratacultural Science, 2012, 29(7): 1105-1113.
孙宗玖, 李培英, 吉鹏飞. 种子萌发期38份偃麦草种质耐盐性评价. 草业科学, 2012, 29(7): 1105-1113.
[8] Ruan Z P, Tang Y J, Zeng M J. Influence of drought stress on photosynthetic characteristics and activity of antioxidant enzymes of four species of palm seedlings. Chinese Journal of Tropical Crops, 2016, 37(10): 1914-1919.
阮志平, 唐源江, 曾美涓. 干旱胁迫对4种棕榈植物幼苗光合特性及抗氧化酶活性的影响. 热带作物学报, 2016, 37(10): 1914-1919.
[9] Lin J X, Li X Y, Tang J H, et al. Effects of salt and alkali stresses on seed germination, early seedling growth and the metabolize of Na+ and K+ in shoots of wheat. Journal of Triticeae Crops, 2011, 31(6): 1148-1152.
蔺吉祥, 李晓宇, 唐佳红, 等. 盐碱胁迫对小麦种子萌发、早期幼苗生长及Na+、K+代谢的影响. 麦类作物学报, 2011, 31(6): 1148-1152.
[10] Ma R J, Jiang Z G. Impact of global climate change on wildlife. Acta Ecologica Sinica, 2005, 25(11): 3061-3066.
马瑞俊, 蒋志刚. 全球气候变化对野生动物的影响. 生态学报, 2005, 25(11): 3061-3066.
[11] Altaisan W A. Comparative effects of drought and salt stress on germination and seedling growth of Pennisetum divisum (Gmel.) Henr. American Journal of Applied Sciences, 2010, 7(5): 640-646.
[12] Zhang X, Lu G, Long W, et al. Recent progress in drought and salt tolerance studies in Brassica crops. Breed Science, 2014, 64(1): 60-73.
[13] Wang J, Meng Y, Li B, et al. Physiological and proteomic analyses of salt stress response in the halophyte Halogeton glomeratus. Plant Cell & Environment, 2015, 38(4): 655-669.
[14] Hu N, Li B C, Yao L R, et al. Effects of different heavy metals on the seed germination and establishment of Halogeton glomeratus. Acta Prataculturae Sinica, 2019, 28(6): 66-81.
胡娜, 李葆春, 姚立蓉, 等. 不同重金属胁迫对盐生草种子萌发特性的影响. 草业学报, 2019, 28(6): 66-81.
[15] Zhang C X, Bai T Q, Xie D H, et al. Effects of PEG-6000 simulation drought stress on seed germination of mango (Mangifer aindica L.). Journal of Southern Agriculture, 2019, 50(3): 600-606.
张翠仙, 柏天琦, 解德宏, 等. PEG-6000模拟干旱胁迫对芒果种子萌发的影响. 南方农业学报, 2019, 50(3): 600-606.
[16] Su M F, Wei X H, Xin X Q, et al. Exogenous cGMP regulates seed germination of ryegrass under salt stress. Acta Ecologica Sinica, 2018, 38(17): 6171-6179.
宿梅飞, 魏小红, 辛夏青, 等. 外源cGMP调控盐胁迫下黑麦草种子萌发机制. 生态学报, 2018, 38(17): 6171-6179.
[17] Liu Y, Jiang H, Zhao Z, et al. Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. Journal of Plant Physiology, 2011, 168(9): 853-862.
[18] Yu J, Li H Y, Li J, et al. Evaluation to salt resistance of two Lotus corniculatus strains in germination period. Acta Agrestia Sinica, 2018, 26(2): 414-419.
于洁, 李鸿雁, 李俊, 等. 2个百脉根品系种子萌发期的耐盐性评价. 草地学报, 2018, 26(2): 414-419.
[19] Andersson S. Paternal effects on seed germination: A barrier to the genetic assimilation of an endemic plant taxon. Journal of Evolutionary Biology, 2010, 21(5): 1408-1417.
[20] Si D J, Yin S P, Zhao X Y, et al. Influences of hormones on seed germination of Tamarix hispida under NaCl stress. Genomics and Applied Biology, 2017, 36(5): 2068-2075.
司冬晶, 尹绍鹏, 赵曦阳, 等. NaCl胁迫下激素对柽柳种子萌发的影响. 基因组学与应用生物学, 2017, 36(5): 2068-2075.
[21] Xu J X, Yang J, Wu J, et al. Effects of simulated drought stress by polyethylene glycol (PEG-6000) on seed germination and seedling growth of Taraxacum kok-saghyz Rodin (TKS) in Xinjiang. Agricultural Research in the Arid Areas, 2015, 33(5): 96-100.
徐建欣, 杨洁, 吴景, 等. 聚乙二醇模拟干旱对新疆橡胶草种子萌发与幼苗生长的影响. 干旱地区农业研究, 2015, 33(5): 96-100.
[22] Li J F, Tie Y H, Wang W, et al. Effect of different salt stress on the seed germination of Halogeton arachnoideus. Journal of Anhui Agricultural Sciences, 2014, 42(18): 5885-5889.
李锦锋, 铁云华, 王文, 等. 不同盐分胁迫对白茎盐生草种子萌发的影响. 安徽农业科学, 2014, 42(18): 5885-5889.
[23] Liu K B, Jiang S X. Responses of Apooceynum venetum seed germination to drought and salt stress. Acta Prataculturae Sinica, 2016, 25(5): 214-221.
刘克彪, 姜生秀. 干旱和钠盐胁迫对罗布麻种子萌发的影响. 草业学报, 2016, 25(5): 214-221.
[24] Yan X F, Sun G R. Salt stress on seed germination of Puccinellia tenuiflora. Pratacultural Science, 1994, 11(4): 27-31.
阎秀峰, 孙国荣. 盐分对星星草种子萌发的胁迫作用. 草业科学, 1994, 11(4): 27-31.
[25] Lin J X, Li Z L, Wang Y N, et al. Effects of various mixed salt-alkaline stress conditions on seed germination and early seedling growth of Leymus chinensis from Songnen grassland of China. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2014, 42(1): 154-159.
[26] Huang G J, Wang B, Yang Z P, et al. Effects of salt stress on biomass and quality of switchgrass. Tianjin Science & Technology, 2019, 46(2): 26-28.
黄高鉴, 王斌, 杨治平, 等. 盐胁迫对柳枝稷生物量及品质的影响. 天津科技, 2019, 46(2): 26-28.
[27] Xiong X, Luo J C, Wei Y Q, et al. Effects of non-uniform salt stress on growth characteristics of alfalfa. Scientia Agricultura Sinica, 2018, 51(11): 2072-2083.
熊雪, 罗建川, 魏雨其, 等. 不均匀盐胁迫对紫花苜蓿生长特性的影响. 中国农业科学, 2018, 51(11): 2072-2083.
[28] Xu N, Cao N, Wang C, et al. Effects of NaCl stress on seed germination and seedling physiological characteristics of wild and cultivated varieties of Sorghum bicolor. Jiangsu Agricultural Sciences, 2018, 46(18): 55-57, 67.
徐宁, 曹娜, 王闯, 等. NaCl 胁迫对野生和栽培品种高粱种子萌发和幼苗生理特性的影响. 江苏农业科学, 2018, 46(18): 55-57, 67.
[29] Yang Z, Chen S Y, Zhou Q P, et al. Effects of exogenous silicon on root morphology of Elymus nutans seedlings under simulant drought stress. Pratacultural Science, 2019, 36(5): 1371-1378.
杨智, 陈仕勇, 周青平, 等. 外源硅添加对模拟干旱胁迫下垂穗披碱草苗期根系形态的影响. 草业科学, 2019, 36(5): 1371-1378.
[30] Tian X X, Mao P C, Li S S, et al. Determination of salt-tolerant indexes and comprehensive evaluation of salt-tolerance at the seedlings of Medicago sativa. Acta Agrestia Sinica, 2017, 25(3): 545-553.
田小霞, 毛培春, 李杉杉, 等. 紫花苜蓿苗期耐盐指标筛选及耐盐性综合评价. 草地学报, 2017, 25(3): 545-553.
[31] Li F X, Zhou Y F, Wang Y T, et al. Screening and identification of sorghum bicolor cultivars for alkali tolerance during germination. Scientia Agricultura Sinica, 2013, 46(9): 1762-1771.
李丰先, 周宇飞, 王艺陶, 等. 高粱品种萌发期耐碱性筛选与综合鉴定. 中国农业科学, 2013, 46(9): 1762-1771.
[32] Yang F, Wei X C, Zhang S C, et al. Comparison on salt and drought resistances of different varieties of Sorghum dochna at germination stage. Plant Physiology Journal, 2015, 51(10): 1604-1610.
杨帆, 魏晓岑, 张士超, 等. 不同甜高粱品种萌发期抗盐和抗旱性比较. 植物生理学报, 2015, 51(10): 1604-1610.
[33] Lin J. Salt tolerance and drought resistance of Secale cereal and Avena sativa during the seed germination stage. China Herbivore Science, 2016, 36(4): 31-34.
蔺军. 黑麦和燕麦种子萌发期耐盐性、抗旱性比较. 中国草食动物科学, 2016, 36(4): 31-34.

干旱和盐胁迫对盐生植物盐生草种子萌发特性的影响

Effects of drought and salt stress on seed germination characteristics of Halogeton glomeratus

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	候怡谣, 李霄, 龙瑞才, 杨青川, 康俊梅, 郭长虹. 过量表达紫花苜蓿MsHB7基因对拟南芥耐旱性的影响[J]. 草业学报, 2021, 30(4): 170-179.
[2]	刘凯强, 刘文辉, 贾志锋, 梁国玲, 马祥. 干旱胁迫对‘青燕1号’燕麦产量及干物质积累与分配的影响[J]. 草业学报, 2021, 30(3): 177-188.
[3]	李冬, 申洪涛, 王艳芳, 王悦华, 王丽君, 赵世民, 刘领. 外源褪黑素对干旱胁迫下烟草幼苗光合碳同化和内源激素的影响[J]. 草业学报, 2021, 30(1): 130-139.
[4]	王苗苗, 周向睿, 梁国玲, 赵桂琴, 焦润安, 柴继宽, 高雪梅, 李娟宁. 5份燕麦材料苗期耐盐性综合评价[J]. 草业学报, 2020, 29(8): 143-154.
[5]	曾令霜, 李培英, 孙晓梵, 孙宗玖. 新疆不同生境狗牙根种质抗旱性综合评价[J]. 草业学报, 2020, 29(8): 155-169.
[6]	张宇君, 尚以顺, 王普昶, 丁磊磊, 张文, 邹超. 干旱胁迫下保水剂对盘江白刺花幼苗生长和生理特性的影响[J]. 草业学报, 2020, 29(7): 90-98.
[7]	王泳超, 张颖蕾, 闫东良, 何灵芝, 李卓, 燕博文, 邵瑞鑫, 郭家萌, 杨青华. 干旱胁迫下γ-氨基丁酸保护玉米幼苗光合系统的生理响应[J]. 草业学报, 2020, 29(6): 191-203.
[8]	李柯, 周庄煜, 李四菊, 姚浩铮, 周莹, 缪雨静, 唐晓清, 王康才. 荆芥的生长、渗透调节和抗氧化能力对干旱胁迫的响应[J]. 草业学报, 2020, 29(5): 150-158.
[9]	高子奇, 王佳, 汤宇晨, 王迎春. 唐古特白刺类黄酮-3-O-葡萄糖基转移酶基因(NtUFGT)的克隆与功能分析[J]. 草业学报, 2020, 29(5): 159-170.
[10]	赵颖, 魏小红, 李桃桃. 外源NO对混合盐碱胁迫下藜麦种子萌发和幼苗生长的影响[J]. 草业学报, 2020, 29(4): 92-101.
[11]	赵小强, 陆晏天, 白明兴, 徐明霞, 彭云玲, 丁永福, 庄泽龙, 陈奋奇, 张大志. 不同株型玉米基因型对干旱胁迫的响应分析[J]. 草业学报, 2020, 29(2): 149-162.
[12]	黄勇, 郭猛, 张红瑞, 周艳, 李贺敏, 高致明, 王盼盼. 盐胁迫对石竹种子萌发和幼苗生长的影响[J]. 草业学报, 2020, 29(12): 105-111.
[13]	王桔红, 史生晶, 陈文, 甘桂媚, 陈赛娜, 李张伟. 枯草芽孢杆菌和3种放线菌对盐胁迫下鬼针草和鳢肠种子萌发及幼苗生长的影响[J]. 草业学报, 2020, 29(12): 112-120.
[14]	许爱云, 曹兵, 谢云. 干旱风沙区煤炭基地12种草本植物对干旱胁迫的生理生态响应及抗旱性评价[J]. 草业学报, 2020, 29(10): 22-34.
[15]	马碧花, 蔺伟虎, 高敏, 王兴迪, 田沛. 干旱胁迫下水杨酸和内生真菌对多年生黑麦草的影响[J]. 草业学报, 2020, 29(1): 135-144.