岷县生荒地和熟地育成当归苗抗逆生理特性的差异

doi:10.11686/cyxb2019292

草业学报 ›› 2019, Vol. 28 ›› Issue (11): 86-95.DOI: 10.11686/cyxb2019292

岷县生荒地和熟地育成当归苗抗逆生理特性的差异

白刚¹, 郭凤霞^1,*, 陈垣^1,2,*, 袁洪超¹, 肖婉君¹

1.甘肃农业大学生命科学技术学院,农学院,甘肃省中药材规范化生产技术创新重点实验室,甘肃省药用植物栽培育种工程研究中心,甘肃省干旱生境作物学重点实验室,甘肃兰州 730070;
2.甘肃省特色药用植物资源保护与利用工程实验室,甘肃省特色药材规范化可追溯栽培工程技术研究中心,甘肃中天药业有限责任公司,甘肃定西 748100

收稿日期:2019-06-24 出版日期:2019-11-20 发布日期:2019-11-20
通讯作者: *. E-mail: guofx@gsau.edu.cn, chenyuan@gsau.edu.cn
作者简介:白刚(1983-),男,甘肃平凉人,在读博士。E-mail: 308996123@qq.com
基金资助:
国家自然科学地区基金(31560175、31360317),甘肃省高等学校科研项目(2017A-033),甘肃农业大学盛彤笙基金(GSAU-STS-1612),省级大学生创新训练(201810733232),甘肃农业大学SRTP(20150802、20160802、20170924),甘肃省现代农业中药材产业体系首席专家(GARS-ZYC-1),国家中药标准化项目(ZYBZH-Y-GS-11)和当归GAP基地建设项目资助

Differences in physiological resistance traits of Angelica sinensis seedlings from uncultivated and cultivated fields in Min County

BAI Gang¹, GUO Feng-xia^1,*, CHEN Yuan^1,2,*, YUAN Hong-chao¹, XIAO Wan-jun¹

1.College of Life Science and Technology, College of Agronomy, Gansu Provincial Key Lab of Good Agricultural Production for Traditional Chinese Medicines, Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China;
2.Gansu Engineering Lab of Resource Reservation and Utilization for Characteristic Medical Plants, Gansu Cultivated Engineering and Technology Research Center of Standardization and Traceability for Characteristic Chinese Medicine, Gansu Zhongtian Pharmaceutical Co., Ltd, Dingxi 748100, China

Received:2019-06-24 Online:2019-11-20 Published:2019-11-20
Contact: *. E-mail: guofx@gsau.edu.cn, chenyuan@gsau.edu.cn

摘要/Abstract

摘要： 当归采用生荒地的传统育苗方式造成生境恶化,熟地连作迎茬使当归栽培成效下降。在当归道地产区甘肃省岷县熟地栽培当归后依次轮作豌豆-黄芪,培育双豆茬口再进行当归育苗。以生荒地育苗为对照,通过测定种苗生理抗逆指标,成药期统计成活率和早期抽薹率,旨在揭示生荒地和双豆作物轮作熟地育成当归苗的抗逆性差异,为探寻适宜当归熟地育苗的轮作茬口提供科学依据和技术指标。结果表明,熟地豌豆-黄芪间茬育成当归苗根系相对电导率、氨基酸泄漏率、可溶性蛋白质和糖含量、丙二醛(MDA)含量、自动氧化速率及超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均与生荒地育成苗的差异性不显著。尽管生荒地育成苗的根系氨基酸泄漏率和自氧速率较低,CAT活性较高,早期抽薹率降低5.32%,但双豆间茬熟地育成苗根系相对电导率更低,SOD和POD活性更高,使MDA维持在较低浓度范围,越冬后根病率下降3.33%,病指下降3.06%,移栽后返青势更强,较生荒地苗返青率提高7.52%,成药率提高11.39%。因此,岷县当归道地产区熟地栽培当归后,经豌豆-黄芪双豆科轮作再行当归育苗可有效改善当归苗的生理抗逆性,减轻根际自毒作用,获得优质种苗,育苗期提倡有机栽培,加强田间管理。

关键词: 当归, 生荒地, 熟地, 育苗, 抗逆性

Abstract: Growing Angelica sinensis seedlings traditionally on uncultivated alpine meadow land causes habitat destruction, but it can show reduced productivity when grown in continuously cropped farmland. In this study, we compared wild-grown A. sinensis seedlings with those grown in pea-Astragalus alternate stubble in farmland in Min County, where is the plant’s geo-authentic habitats. Physiological indicators were measured at the end of the seedling stage, and the survival and early bolting rates were determined to evaluate differences in resistance between seedlings from uncultivated and cultivated fields. The results showed that there were no significant differences in relative conductivity, amino acid leakage rate, soluble protein and sugar contents, malondialdehyde content, autoxidation rate as well as superoxide dismutase, peroxidase, and catalase activities between the seedlings from the two fields. Compared with the field-grown seedlings, those from uncultivated field showed relatively lower amino acid leakage but higher catalase activity, leading to a 5.32% lower early bolting rate. Compared with the seedlings from uncultivated field, those grown in farmland exhibited lower relative conductivity, higher superoxide dismutase and peroxidase activities, lower malondialdehyde content, 3.33% lower incidence of root disease, 3.06% lower overwintering disease index, 7.52% higher re-greening power after transplantation, and 11.39% higher ratio of medicinal plants. Hence, seedlings of A. sinensis in Min County showed better resistance and lower autotoxicity when cultivated in a double-legume stubble field. Field management should be enhanced to include organic cultivation during the seedling-raising period. These findings provide a scientific and technical basis for the cultivation of A. sinensis seedlings as part of a field rotation system.

Key words: Angelica sinensis, uncultivated field, cultivated field, seedling cultivation, stress resistance

白刚, 郭凤霞, 陈垣, 袁洪超, 肖婉君. 岷县生荒地和熟地育成当归苗抗逆生理特性的差异[J]. 草业学报, 2019, 28(11): 86-95.

BAI Gang, GUO Feng-xia, CHEN Yuan, YUAN Hong-chao, XIAO Wan-jun. Differences in physiological resistance traits of Angelica sinensis seedlings from uncultivated and cultivated fields in Min County[J]. Acta Prataculturae Sinica, 2019, 28(11): 86-95.

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岷县生荒地和熟地育成当归苗抗逆生理特性的差异

Differences in physiological resistance traits of Angelica sinensis seedlings from uncultivated and cultivated fields in Min County

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