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草业学报 ›› 2014, Vol. 23 ›› Issue (5): 168-174.DOI: 10.11686/cyxb20140519

• 论文 • 上一篇    下一篇

海滨雀稗液泡膜H+-PPase(PvVP1)5'端的克隆和序列分析

宋辉1,南志标1*,蔡小宁2,钟小仙3,顾洪如3   

  1. 1.草地农业生态系统国家重点实验室 兰州大学草地农业科技学院,甘肃 兰州 730020;
    2.南京晓庄学院生物化工与环境工程学院,江苏 南京 211171;
    3.江苏省农业科学院畜牧研究所,江苏 南京 210014
  • 收稿日期:2012-08-30 出版日期:2014-10-20 发布日期:2014-10-20
  • 通讯作者: Email:zhibiao@lzu.edu.cn
  • 作者简介:宋辉(1985-),男,山东鱼台人,在读博士。E-mail:songh12@lzu.edu.cn
  • 基金资助:

    国家“973”项目(2014CB138702)资助

Clone and sequence analysis of 5’RACE of PvVP1 in Paspalum vaginatum

SONG Hui1,NAN Zhi-biao1,CAI Xiao-ning2,ZHONG Xiao-xian3,GU Hong-ru3   

  1. 1.State Key Laboratory of Grassland Agro-Ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China;
    2.School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China;
    3.Institute of Animal Science, Jiangsu Academy of Agricultural Science, Nanjing 210014, China
  • Received:2012-08-30 Online:2014-10-20 Published:2014-10-20

摘要:

根据已公布液泡膜H+-PPase基因家族同源序列保守区设计简并引物,克隆出海滨雀稗中PvVP1基因的中间序列,然后用快速扩增cDNA末端(rapid amplification of cDNA end, RACE)方法, 从海滨雀稗中克隆到PvVP1 5'cDNA序列。该序列ORF长1605 bp,编码535个氨基酸,其编码序列、氨基酸序列与玉米相应基因的同源性分别为93%和99%。

Abstract:

A pair of degenerated primers were designed based on previously cloned homologous cDNA segments. The middle segment of PvVP1 was cloned, then the PvVP1 5’cDNA sequence also obtained using 5’RACE. The open reading frame (ORF) of this sequence was 1605 bp, encoding 534 amino acids. The codon and amino acid sequences of the 5’ends of the PvVP1 gene shared 93% and 99% similarity, respectively, with those of Zea mays.

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