Abstract: Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 68 accessions of Elymus nutans collected from Gansu, Sichuan, Tibet, Qinghai, and Xinjiang areas of China. Twenty primer pairs produced 495 bands (average of 24.75 bands per primer pair), including 425 polymorphic bands (21.25 bands per primer pair). The average percentage of polymorphic bands was 85.39%. The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.374 to 0.997, and the average Nei’s coefficient was 0.745. These Results suggest that there was a rich genetic diversity among the resources of Elymus nutans tested. The 68 accessions can be clustered into five dendrogram groups at the GS=0.77 level. The principal coordinates (PCA) reflected almost the same relationships among the studied materials as found in cluster analysis. Moreover, the accessions from the same origin frequently clustered into one group. The findings indicated a correlation among the wild resources and their geographical and ecological environments.