Na+对渗透胁迫下霸王幼苗光合特性的影响

草业学报 ›› 2010, Vol. 19 ›› Issue (3): 198-203.

Na⁺对渗透胁迫下霸王幼苗光合特性的影响

马清,楼洁琼,王锁民^*

兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2009-04-28 出版日期:2010-03-25 发布日期:2010-06-20
作者简介:马清(1986-),男,甘肃天水人,在读博士。E-mail:maq03@lzu.cn
基金资助:
“十一五”国家科技支撑计划重点项目(2008BADB3B01),863 计划(2006AA10Z126)和国家自然科学基金(30770347,30671488)资助。

Effect of Na⁺ on photosynthetic characteristics of Zygophyllum xanthonylom seedlings under osmotic stress

MA Qing, LOU Jie-qiong, WANG Suo-min

School of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2009-04-28 Online:2010-03-25 Published:2010-06-20

摘要/Abstract

摘要： 在-0.5MPa渗透胁迫下,研究了Na⁺对多浆旱生植物霸王幼苗光合特性的影响。结果显示,在渗透胁迫下,50mmol/LNaCl的加入使霸王幼苗的叶面积和叶绿素(a+b)含量分别显著增加了105%和33%,净光合速率(Pn)、气孔导度(Gs)以及磷酸烯醇式丙酮酸羧化酶(PEPCase)活性分别显著提高了115%,90%和180%,而胞间CO₂浓度(Ci)显著降低了17%;同时,使PSⅡ的潜在活性(F_v/F₀)、原初光能转换效率(F_v/F_m)以及光合电子传递速率(ETR)分别显著提高了15%,5%和11%。以上结果表明,Na⁺能改善渗透胁迫下霸王幼苗的光合作用,从而提高植株的抗胁迫能力。

Abstract: The effect of Na⁺ on the photosynthetic characteristics of seedlings of the succulent xerophyte Zygophyllum xanthoxylum were investigated under -0.5 MPa osmotic stress. Under this stress, the leaf area, chlorophyll (a+b) content, net photosynthetic rate (Pn), stomatal conductance (Gs) and phosphoenolpyruvate carboxylase (PEPCase) activity were significantly elevated by 105%, 33%, 115%, 90%, and 180% respectively at 50 mmol/L NaCl, but the intercellular CO₂ concentration (Ci) was significantly reduced by 17%. In addition, the potential photosynthetic activity (F_v/F₀), primary light energy conversion efficiency (F_v/F_m), and photosynthetic electron transport rate (ETR) of PSⅡ were enhanced by 15%, 5%, and 11% respectively. These results indicate that, under -0.5 MPa osmotic stress, Na⁺ can improve the photosynthesis of Z. xanthoxylum seedlings and enhance the osmotic stress resistance of plants.

中图分类号:

Q945.78

马清,楼洁琼,王锁民. Na⁺对渗透胁迫下霸王幼苗光合特性的影响[J]. 草业学报, 2010, 19(3): 198-203.

MA Qing, LOU Jie-qiong, WANG Suo-min. Effect of Na⁺ on photosynthetic characteristics of Zygophyllum xanthonylom seedlings under osmotic stress[J]. Acta Prataculturae Sinica, 2010, 19(3): 198-203.

参考文献

[1] Zhu X Y, Chen G C, Zhang C L. Photosynthetic electron transport, photophosphorylation and antioxidants in two ecotypes of reed (Phragmtes communis Trin.) from different habitats[J]. Photosynthetica, 2001, 39: 183-189.
[2] 李三相, 周向睿, 王锁民. Na⁺在植物中的有益作用[J]. 中国沙漠, 2008, 28(3): 485-490.
[3] Murata S, Sekiya J. Effects of sodium on photosynthesis in Panicum coloratum[J]. Plant Cell Physiology, 1992, 33: 1239-1242.
[4] Ohnishi J, Flugge U, Heldt H W, et al. Involvement of Na⁺ in active uptake of pyruvate in mesophyll chloroplasts of some C₄ plant[J]. Plant Physiology, 1990, 94: 950-959.
[5] 赵一之, 朱宗元. 亚洲中部荒漠区的植物特有属[J]. 云南植物研究, 2003, 25(2): 113-121.
[6] 杨鑫光, 傅华, 牛得草. 干旱胁迫下幼苗期霸王的生理响应[J]. 草业学报, 2007, 16(5): 107-112.
[7] Wang S M, Wan C G, Wang Y R. The characteristics of Na⁺, K⁺ and free proline distribution in several drought resistant plants of the Alxa Desert, China[J]. Journal of Arid Environments, 2004, 56: 525-539.
[8] 李三相. Na⁺与多浆旱生植物霸王抗旱性研究[D]. 兰州: 兰州大学, 2006.
[9] Martinez J P, Kinet J M, Bajji M, et al. NaCl alleviates polyethylene glycol-induced water stress in the halophyte species Atriplex halimus L[J]. Journal of Experimental Botany, 2005, 56: 2421-2431.
[10] Debez A, Saadaoui D, Ramani B, et al. Leaf H⁺-ATPase activity and photosynthetic capacity of Cakile maritime under increasing salinity[J]. Environmental and Experimental Botany, 2006, 57: 285-295.
[11] Slama I, Ghnaya T, Messedi D, et al. Effect of sodium chloride on the response of the halophyte species Sesuvium portulacastrum grown in mannitol-inducedwater stress[J]. Journal of Plant Research, 2007, 120: 291-299.
[12] 沈伟其. 测定水稻叶片叶绿素含量的混合液提取法[J]. 植物生理学通讯, 1988, 3: 62-64.
[13] 李卫华, 张承烈. 泡泡刺叶磷酸烯醇式丙酮酸羧化酶季节性聚态变化[J]. 植物生态学报, 2000, 24(3): 284-288.
[14] Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochemica et Biophysica Acta, 1989, 990: 87-92.
[15] 张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学通报, 1999, 16(4): 444-448.
[16] 赵博生, 衣艳君, 刘家尧. 外源甜菜碱对干旱/盐胁迫下的小麦幼苗生长和光合功能的改善[J]. 植物学通报, 2001, 18(3): 378-380.
[17] Powle S B. Photo inhibition of photosynthesis induced by visible light[J]. Annual Review of Plant Physiology, 1984, 35: 15-44.
[18] Farquhar G D, Sharkey T D. Stomatal conductance and photosynthesis[J]. Annual Review of Plant Physiology, 1982, 33: 317-345.
[19] 陈建明, 俞晓平, 程家安. 叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J]. 浙江农业学报, 2006, 18(1): 51-55.
[20] Lu C M, Qiu N W, Wang B S, et al. Salinity treatment shows no effects on photosystem II photochemistry, but increases the resistance of photosystem II to heat stress in halophyte Suaeda salsa[J]. Journal of Experimental Botany, 2003, 54: 851-860.
[21] Cheeseman J M. Mechanism of salinity tolerance in plants[J]. Plant Physiology, 1988, 87: 547-550.
[22] Brownell P F, Bielig L M. The role of sodium in the conversion of pyruvate to phosphoenolpyruvate in mesophyll chloroplasts of C₄ plants[J]. Australian Journal of Plant Physiology, 1996, 23: 171-177.
[23] 吴雪霞, 朱为民, 朱月林, 等. 外源一氧化氮对NaCl胁迫下番茄幼苗光合特性的影响[J]. 植物营养与肥料学报, 2007, 13(6): 1105-1109.
[24] 李卫华, 郝乃斌, 戈巧英, 等. C₃植物中C₄途径的研究进展[J]. 植物学通报, 1999, 16(2): 97-106.
[25] Ting I P, Osmond C B. Photosynthetic photosynthetic phosphoenolpyruvate carboxylase characteristics of alloenzymes from leaves of C₃ and C₄ plants[J]. Plant Physiology, 1973, 51: 439-447.
[26] Stitt M. Nitrate regulation of metabolism and growth[J]. Current Opinion in Plant Biology, 1999, 2: 178-186.