SRAP与SSR分子标记在柳枝稷杂种鉴定中的比较分析

草业学报 ›› 2012, Vol. 21 ›› Issue (5): 128-133.

SRAP与SSR分子标记在柳枝稷杂种鉴定中的比较分析

张婧¹,黄琳凯^1*,ZHAO Bing-yu²,张新全¹,严海东¹,蒋晓阳¹

1.四川农业大学草业科学系,四川雅安625014;
2.弗吉尼亚理工大学,美国弗吉尼亚州VA 24061

收稿日期:2011-10-24 出版日期:2012-05-25 发布日期:2012-10-20
通讯作者: E-mail:huanglinkai2000@yahoo.com.cn
作者简介:张婧(1989-),女,山西原平人,在读本科。E-mail:zhangjing1924@163.com
基金资助:
“十二五”农村领域国家科技计划课题(2012AA101801-02)和教育部博士点新教师基金(20115103120004)资助。

Comparison of SRAP and SSR markers for hybrid identification of switchgrass (Panicum virgatum)

ZHANG Jing¹, HUANG Lin-kai¹, ZHAO Bing-yu², ZHANG Xin-quan¹, YAN Hai-dong¹, JIANG Xiao-yang¹

1.Department of Grassland Science, Sichuan Agricultural University, Ya’an 625014, China;
2.Virginia Polytechnic Institute and State University, Blacksburg VA24061, America

Received:2011-10-24 Online:2012-05-25 Published:2012-10-20

摘要/Abstract

摘要： 利用SRAP标记和SSR标记对柳枝稷杂交种进行鉴定,比较2种标记在柳枝稷杂种鉴定方面的效率和准确性,为柳枝稷乃至异花授粉多倍体植物的杂种真实性鉴定提供科学依据。分别利用SRAP与SSR标记技术对柳枝稷165个杂交种单株和亲本DNA进行扩增,分析杂交种与亲本扩增的多态性条带数,并鉴别真假杂交种。与SRAP标记相比,共显性的SSR标记具有高效性,本研究中仅用1对SSR引物就鉴别了所有杂交后代的真实性,多态性条带比率为100%。而显性SRAP标记用了6对才能鉴别所有杂交后代的真实性,多态性条带比率为40%,相对较低。

Abstract: To provide a scientific basis for hybrid identification of switchgrass (Panicum virgatum) and cross-pollinating plants, we compared the identification efficiency and accuracy of SRAP and SSR markers. Switchgrass hybrid individuals (165) and their parental lines of switchgrass were tested by both methods. Compared to SRAP markers, the codominant SSR technique was more efficient. One pair of SSR primers (primer 23) detected all hybrids effectively and the percentage of polymorphic bands was 100%. SRAP required six primers to identify hybrids. The percentage of polymorphic bands of SRAP was 40% lower than that of SSR.

中图分类号:

Q943

张婧,黄琳凯,ZHAO Bing-yu,张新全,严海东,蒋晓阳. SRAP与SSR分子标记在柳枝稷杂种鉴定中的比较分析[J]. 草业学报, 2012, 21(5): 128-133.

ZHANG Jing, HUANG Lin-kai, ZHAO Bing-yu, ZHANG Xin-quan, YAN Hai-dong, JIANG Xiao-yang. Comparison of SRAP and SSR markers for hybrid identification of switchgrass (Panicum virgatum)[J]. Acta Prataculturae Sinica, 2012, 21(5): 128-133.

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