盐胁迫对碱地风毛菊苗期PM-ATPase及5′-AMPase活性的影响

草业学报 ›› 2012, Vol. 21 ›› Issue (2): 156-161.

盐胁迫对碱地风毛菊苗期PM-ATPase及5′-AMPase活性的影响

李鹏飞¹,杜海燕²,夏方山³,董秋丽³,董宽虎^3*

1.山西农业大学生命科学学院,山西太谷 030801;
2.山西农业大学研究生学院,山西太谷 030801;
3.山西农业大学动物科技学院,山西太谷 030801

收稿日期:2011-03-04 出版日期:2012-02-25 发布日期:2012-04-20
通讯作者: E-mail:dongkuanhu@126.com
作者简介:李鹏飞(1978-),男,山西偏关人,在读博士。 E-mail:adamlpf@126.com
基金资助:
国家“十一五”科技支撑计划项目(2007BAD56B01)资助。

Effects of salt stress on the activity of PM-ATPase and 5′-AMPase of Saussurea runcinata at seedling stage

LI Peng-fei¹, DU Hai-yan², XIA Fang-shan³, DONG Qiu-li³, DONG Kuan-hu³

1.College of Life Science, Shanxi Agricultural University, Taigu 030801, China;
2.Graduate College, Shanxi Agricultural University, Taigu 030801, China;
3.College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Received:2011-03-04 Online:2012-02-25 Published:2012-04-20

摘要/Abstract

摘要： 盐胁迫是影响植物生长、发育以至产量的重要因素,试验通过对碱地风毛菊苗期分别以NaCl和Na₂CO₃进行胁迫,Na⁺浓度为0(CK),60,120,180,240,300,360 mmol/L,测定其丙二醛、PM-ATPase活性及5′-AMPase活性,以探讨盐胁迫对其膜系统的影响及其苗期耐盐性。结果表明,盐胁迫下,碱地风毛菊丙二醛含量增大,Na₂CO₃胁迫下,碱地风毛菊根系和叶片中MDA含量在Na⁺浓度为360 mmol/L时达到最大值,分别为对照的2.15和1.94倍;在NaCl胁迫下,碱地风毛菊叶片中MDA含量最大为对照的1.22倍。同一盐胁迫下,碱地风毛菊根系中PM-ATPase活性和5′-AMPase活性显著高于叶片(P<0.05),碱地风毛菊根系中PM-ATPase活性最大为56.29 μg磷酸/(mg蛋白·h), 而5′-AMPase活性最大为53.91 μg磷酸/(mg蛋白·h)。2种盐胁迫下PM-ATPase和5′-AMPase活性差异显著。总之,盐胁迫下,碱地风毛菊根系比叶片受到的损害小,碱地风毛菊更耐NaCl胁迫。

Abstract: Salt stress affects plant growth and development and reduces productivity of crops. This experiment was carried out to determine the MDA, plasma membrane ATP enzyme (PM-ATPase) and 5′-neucleotidase (5′-AMPase) of Saussurea runcinata seedling by NaCl and Na₂CO₃ stress (The concentration of Na⁺ is 0, 60, 120, 180, 240, 300 and 360 mmol/L) in order to assess the effects of its membrane systemthe under salinity and salt tolereance about it. It has been shown that the MDA increased by salinity stress. The content of MDA in roots and leaves of S. runcinata reached the maximum under Na₂CO₃ stress in the Na⁺ concentration of 360 mmol/L, respectively, 2.15 times and 1.94 times than the control. The maximum content of MDA in leaves of S. runcinata 1.22 times than the control. The activity of PM-ATPase and 5′-AMPase in the roots is higher than those in the leaves under the same concentration of Na⁺. The activity of PM-ATPase and 5′-AMPase were significantly difference by two different salinity stress. The maximum activity of PM-ATPase in the roots of S. runcinata up to 56.29 μg pi/(mg protein·h),but the activity of 5′-AMPase up to 53.91 μg pi/(mg protein·h). In a word, the damage of the roots of the S. runcinata is smaller than the leaves under the salinity stress, the S. runcinata more resistant to NaCl stress.

中图分类号:

Q945.78

李鹏飞,杜海燕,夏方山,董秋丽,董宽虎. 盐胁迫对碱地风毛菊苗期PM-ATPase及5′-AMPase活性的影响[J]. 草业学报, 2012, 21(2): 156-161.

LI Peng-fei, DU Hai-yan, XIA Fang-shan, DONG Qiu-li, DONG Kuan-hu. Effects of salt stress on the activity of PM-ATPase and 5′-AMPase of Saussurea runcinata at seedling stage[J]. Acta Prataculturae Sinica, 2012, 21(2): 156-161.

参考文献

[1] 康建军, 王锁民, 赵明, 等. 苗期施用钠复合肥增强梭梭抗逆性的初步研究[J]. 草业学报, 2011, 20(2): 127-133.
[2] 张军, 王建波, 陈刚, 等. Na₂CO₃胁迫下星星草幼苗叶片电解质外渗率与PSⅡ光能耗散的关系[J]. 草业学报, 2009, 18(3): 200-206.
[3] 陶雅, 玉柱, 孙启忠, 等. 紫花苜蓿的抗寒生理适应性研究[J]. 草业科学, 2009, 26(9): 151-155.
[4] Briiggemann W, Janiesch P. Properties of native and solubilized plasma membrane ATPase from the halophyte Plantago crassfiolia, grown under saline and non-saline conditions[J]. Physiologia Plantarum, 1988, 74: 615-622.
[5] Shi H Z, Quintero F G, Pardo J M, et al. The putative plasma membrane Na⁺/H⁺ antiporter SOS1 controls long-distance Na⁺transport in plants[J]. Plant Cell, 2002, 14: 465-477.
[6] Sgherri C L, Maffei M, Navari I F. Antioxidative enzymes in wheasubjected to increasing water deficit and rewatering[J]. Journal of Plant Physiology, 2000, 157(3): 273-279.
[7] 董秋丽, 夏方山, 董宽虎. NaCl胁迫对芨芨草苗期脯氨酸代谢的影响[J]. 草业学报, 2010, 19(5): 71-76.
[8] Health R L, Packer L. Photoperoxidation in isolated chloroplasts.I kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics, 1968, 12(5): 189-198.
[9] Malgorzata J R, Grazyna K. Modification of plasma membrane and vacuolar H⁺-ATPases in response to NaCl and ABA[J]. Journal of Plant Physiology, 2007, 164: 295-302.
[10] Bradford M M. A rapid and sensitive method for the qantitation of microgram quantities of protein utilizing the principle of protein dye binding[J]. Analytic Biochemistry, 1976, 72: 248-254.
[11] Ames B N. Assay of inorganic phosphate, total phosphate and phosphatases[J]. Methods Enzymol, 1966, 8: 115-118.
[12] 邵艳军, 李广敏, 黄国存, 等. 水分胁迫对冬小麦愈伤组织质膜ATP酶和5′-核苷酸酶活性的影响[J]. 干旱地区农业研究, 2000, 18(3): 93-97.
[13] 潘杰, 孙龙华, 简令成. 不同抗冷性水稻质膜5′-核苷酸酶活性的生化及细胞化学研究[J]. 科学通报, 1992, 28(7): 653-658.
[14] 邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2005: 173-174.
[15] 赵茁萌, 黄军, 石定燧, 等. 鲁梅克斯K-1杂交酸模耐盐性的初步研究[J]. 中国草地, 2000, (1): 26-30.
[16] 冯建永, 庞民好, 刘颖超, 等. 复杂盐碱对黄顶菊种子萌发和幼苗生长的影响及机理初探[J]. 草业学报, 2010, 19(5): 77-86.
[17] 龚明, 赵方杰, 吴颂如, 等.NaCl胁迫对大麦硝酸盐吸收和有关酶活性的影响[J]. 植物生理学报, 1990, (2): 13-16.
[18] 肖雯, 贾恢先, 蒲陆梅. 几种盐生植物抗盐生理指标的研究[J]. 西北植物学报, 2000, 20(5): 818-825.
[19] 夏方山, 董秋丽, 董宽虎. 碱胁迫对碱地风毛菊生理特性的影响[J]. 中国农学通报, 2010, 26(21): 152-155.
[20] 陈敏, 王宝山. 植物质膜H⁺-ATPase响应盐胁迫的分子机制[J]. 植物生理学通讯, 2006, 42(5): 807-811.
[21] Roy P, Niyogi K, Ghosh B S, et al. permidine treatmentto rice seedlings recovers salinity stress-induced damage of plasma membrane and PM-bound H⁺-ATPase in salt-tolerant and salt-sensitive rice cultivars[J]. Plant Science, 2005, 168: 583-591.
[22] Zhu J K. Genetic analysis of plant salt tolerance using Arabidopsis[J]. Plant Physiology, 2000, 124: 941-948.
[23] 杨颖丽, 杨宁, 安黎哲, 等. 植物质膜H⁺-ATPase的研究进展[J]. 西北植物学报, 2006, 26(11): 2388-2396.
[24] Yamashita K, Matsumoto H. Effect of sodium chloride stress on the plasma membrane ATPase of braley roots: probable cause for decrease in ATPase activity[J]. Journal of Plant Nutrition, 1997, 20: 233-245.
[25] 章文华, 陈沁, 刘友良. NaCl胁迫下大麦根系液泡膜H⁺-ATPase活性与膜流动性的关系[J]. 植物学报, 2002, 44(3): 292-296.
[26] 张健强, 张建光, 付艾莉, 等. 外源抗氧化剂对高温胁迫下苹果果皮组织酶活性的影响[J]. 华北农学报, 2005, 20(2): 41-44.
[27] 马丽贤, 董宽虎. 盐碱胁迫对华蒲公英质膜ATP酶和5′-核苷酸酶的影响[J]. 草地学报, 2010, 18(4): 556-559.