基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征

doi:10.11686/cyxb2019337

摘要/Abstract

摘要： 荒漠草地占新疆草地总面积的46.9%,不同荒漠类型的气候差异显著,植被和土壤类型也各不相同。以准噶尔盆地大面积分布的3种代表性荒漠植被类型(南缘伊犁绢蒿荒漠、腹地白梭梭荒漠和北缘盐生假木贼荒漠)为研究对象,应用高通量测序技术,分析比较3种荒漠植被类型的土壤细菌群落组成和多样性特征。结果表明:放线菌门、变形菌门、绿弯菌门、酸杆菌门、芽单胞菌门和拟杆菌门是准噶尔盆地荒漠土壤中6个主要优势类群细菌,其相对丰度累计超过94%。放线菌门在3种荒漠类型中相对丰度最高,均超过50%,但差异不显著(P>0.05);酸杆菌门在伊犁绢蒿荒漠中相对丰度最高,厚壁菌门在白梭梭荒漠丰度最高,拟杆菌门在盐生假木贼荒漠丰度最高,且这3种菌门在3种荒漠类型中差异显著(P<0.05);在3种荒漠类型中,白梭梭荒漠细菌操作分类单元(OTU)数最少,多样性最低。Pearson相关性分析和RDA分析结果均表明,年均降水量和土壤有机碳是影响细菌群落结构组成和多样性最显著的环境因子。

关键词: 准噶尔荒漠, 高通量测序, 土壤细菌, 土壤养分

Abstract: Desert grassland accounts for 46.9% of the total grassland area in Xinjiang, with different desert types in areas of different climate, each with significantly different vegetation and soil types. Three representative desert vegetation types: Seriphidium transiliense desert in the southern margin, Haloxylon persicum desert in the hinterland and Anabasis salsa desert in the northern margin of the Junggar Basin, were chosen for study. The bacterial community composition and diversity characteristics of the three desert vegetation types were analyzed and compared using a high-throughput sequencing technique. It was found that Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, Gemmatimonadetes, and Bacteroidetes were the six dominant groups of bacteria in these Junggar Basin soils. The cumulative abundance was more than 94%. Actinobacteria had the highest relative abundance (>50%) in all three desert types, and the difference between desert types for relative abundance of Actinobacteria was not significant (P>0.05). Acidobacteria had a relative abundance in S. transiliense desert notably higher than in other desert types, while Firmicutes bundance was higher in H. persicum desert than in other desert types, and Bacteroidetes had the highest relative abundance in A. salsa desert, and these differences were significant (P<0.05). Among the three desert types, the number of bacterial operational taxonomic units in H. persicum desert was the lowest and the community diversity was the lowest. Pearson correlation and redundancy analyses showed that average annual precipitation and soil organic carbon are the most significant environmental factors affecting the composition and diversity of the bacterial communities studied.

Key words: Junggar desert, high-throughput sequencing, soil bacteria, soil nutrients

魏鹏, 安沙舟, 董乙强, 孙宗玖, 别尔达吾列提·希哈依, 李超. 基于高通量测序的准噶尔盆地荒漠土壤细菌多样性及群落结构特征[J]. 草业学报, 2020, 29(5): 182-190.

WEI Peng, AN Sha-zhou, DONG Yi-qiang, SUN Zong-jiu, Bieerdawulieti·Xihayi, LI Chao. A high-throughput sequencing evaluation of bacterial diversity and community structure of the desert soil in the Junggar Basin[J]. Acta Prataculturae Sinica, 2020, 29(5): 182-190.

参考文献

[1] Xu P. Grassland resources and their utilization in Xinjiang. Urumqi: Xinjiang Science and Technology and Health Publishing House, 1993.
许鹏. 新疆草地资源及其利用. 乌鲁木齐: 新疆科技卫生出版社, 1993.
[2] Ren X X. The methodology of desert ecosystem services in monitoring and assessment research. Beijing: Chinese Academy of Forestry, 2012.
任晓旭. 荒漠生态系统服务功能监测与评估方法学研究. 北京: 中国林业科学研究院, 2012.
[3] Whitman W B, Coleman D C, Wiebe W J. Prokaryotes: The unseen majority. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(12): 6578-6583.
[4] Morgan J A W, Bending G D, White P J. Biological costs and benefits to plant-microbe interactions in the rhizosphere. Journal of Experimental Botany, 2005, 56(417): 1729-1739.
[5] Yang J, Zhou G Y, Tian Y Y, et al. Differential analysis of soil bacteria diversity in different mixed forests of Dalbergia odorifera. Acta Ecologica Sinica, 2015, 35(24): 8117-8127.
杨菁, 周国英, 田媛媛, 等. 降香黄檀不同混交林土壤细菌多样性差异分析. 生态学报, 2015, 35(24): 8117-8127.
[6] Van Veen J A, Ladd J N, Amato M. Turnover of carbon and nitrogen through the microbial biomass in a sandy loam and a clay soil incubated with [¹⁴C(U)] glucose and [¹⁵N] (NH₄)₂SO₄ under different moisture regimes. Soil Biology and Biochemistry, 1985, 17(6): 747-756.
[7] Schutter M E, Dick R P. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities. Soil Science Society of America Journal, 2000, 64(5): 1659-1668.
[8] Balser T C, Firestone M K. Linking microbial community composition and soil processes in a California annual grassland and mixed-conifer forest. Biogeochemistry, 2005, 73: 395-415.
[9] Liu J H, Wang Z W, Hao D Y, et al. Effect of heavy grazing on the organization ability of main plant species and functional groups in a desert steppe. Chinese Journal of Grassland, 2018, 40(5): 85-92.
刘菊红, 王忠武, 郝敦元, 等. 重牧对荒漠草原主要植物种和功能群组织力的影响. 中国草地学报, 2018, 40(5): 85-92.
[10] Dong Y Q, Sun Z J, An S Z, et al. Effect of short-term grazing exclusion on community characteristics and stability in Artemisia desert on the northern slopes of the Tianshan Mountains. Pratacultural Science, 2018, 35(5): 996-1003.
董乙强, 孙宗玖, 安沙舟, 等. 短期禁牧对天山北坡蒿类荒漠群落特征及其稳定性的影响. 草业科学, 2018, 35(5): 996-1003.
[11] Jiang S S, Sun Z J, Yang J, et al. Effect of exclusion duration on the plant inter-specific relationship and community stability in Seriphidium transiliense desert grassland. Chinese Journal of Grassland, 2018, 40(3): 68-75.
江莎莎, 孙宗玖, 杨静, 等. 封育年限对伊犁绢蒿荒漠草地群落种间关系及稳定性的影响. 中国草地学报, 2018, 40(3): 68-75.
[12] Guo H C, Yan C, Wei Y. Reproductive phenology and fruit-set pattern of Zygophyllum macroperum in the Junggar Desert, Xinjiang. Acta Ecologica Sinica, 2015, 35(17): 5738-5744.
郭红超, 严成, 魏岩. 准噶尔荒漠大翅霸王的生殖物候及结实格局. 生态学报, 2015, 35(17): 5738-5744.
[13] Cao Y F, Li Y, Li C H, et al. The spatial distribution of soil microbes around a desert shrub of Haloxylon ammodendron. Acta Ecologica Sinica, 2016, 36(6): 1628-1635.
曹艳峰, 李彦, 李晨华, 等. 荒漠灌木梭梭(Haloxylon ammodendron)周围土壤微生物的空间分布. 生态学报, 2016, 36(6): 1628-1635.
[14] Lü G F, Wu Y S, Li H, et al. Study on soil microbe quantities and enzyme activities of different desert steppe in Inner Mongolia. Journal of Inner Mongolia Normal University (Natural Science Edition), 2008, 37(6): 761-764.
吕桂芬, 吴永胜, 李浩, 等. 内蒙古不同类型荒漠草原土壤微生物数量及其酶活性研究. 内蒙古师范大学学报(自然科学汉文版), 2008, 37(6): 761-764.
[15] China Vegetation Map Committee of the Chinese Academy of Sciences. 1/1000000 vegetation map of the People’s Republic of China. Beijing: Geological Publishing House, 2007.
中国科学院中国植被图编辑委员会. 1∶1000000中华人民共和国植被图. 北京: 地质出版社, 2007.
[16] Xinjiang Comprehensive Investigation Team, The Chinese Academy of Sciences, Institute of Botany, The Chinese Academy of Sciences. Vegetation and utilization in Xinjiang. Beijing: Science Press, 1978.
中国科学院新疆综合考察队, 中国科学院植物研究所. 新疆植被及其利用. 北京: 科学出版社, 1978.
[17] Lu R K. Soil agrochemical analysis. Beijing: China Agricultural Science and Technology Press, 2000.
鲁如坤. 土壤农化分析方法. 北京: 中国农业科学技术出版社, 2000.
[18] Zhou J Z, Bruns M A, Tiedje J M. DNA recovery from soils of diverse composition. Applied and Environmental Microbiology, 1996, 62(2): 316-322.
[19] Caporaso J G, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 2010, 7(5): 335-336.
[20] Blaxter M, Mann J, Chapman T, et al. Defining operational taxonomic units using DNA barcode data. Philosophical Transactions of the Royal Society B-Biological Sciences, 2005, 360: 1935-1943.
[21] Zhu J Z. Grassland resources. Beijing: China Agriculture Press, 2010.
朱进忠. 草地资源学. 北京: 中国农业出版社, 2010.
[22] Yin C H, Feng G, Tian C Y, et al. Variations of the fertile island effects beneath tamarisk in northern Taklamakan Desert, northwestern China and its implication to desertification process. Journal of Beijing Forestry University, 2008, 30(1): 52-57.
尹传华, 冯固, 田长彦, 等. 塔克拉玛干沙漠北缘柽柳灌丛肥岛效应的变化规律及其生态学意义. 北京林业大学学报, 2008, 30(1): 52-57.
[23] Wu X D. Effect of grassland desertification on soil organic carbon stability and community succession of desert steppe in Ningxia. Yinchuan: Ningxia University, 2016.
吴旭东. 沙漠化对草地植物群落演替及土壤有机碳稳定性的影响. 银川: 宁夏大学, 2016.
[24] Xu X Y, Yan P, Guo S J. The interception loss of rainfall by three sand-fixing shrubs at the fringe of Minqin Oasis. Journal of Desert Research, 2013, 33(1): 141-145.
徐先英, 严平, 郭树江. 干旱荒漠区绿洲边缘典型固沙灌木的降水截留特征. 中国沙漠, 2013, 33(1): 141-145.
[25] Ding X J, Huang Y L, Jing R Y, et al. Bacterial structure and diversity of four plantations in the Yellow River Delta by high-throughput sequencing. Acta Ecologica Sinica, 2018, 38(16): 5857-5864.
丁新景, 黄雅丽, 敬如岩, 等. 基于高通量测序的黄河三角洲4种人工林土壤细菌结构及多样性研究. 生态学报, 2018, 38(16): 5857-5864.
[26] Bachar A, Al-Ashhab A, Soares MI, et al. Soil microbial abundance and diversity along a low precipitation gradient. Microbial Ecology, 2010, 60(2): 453-461.
[27] Wang X B. The spatial pattern of soil microbial communities and its driving mechanism in the grassland of Northern China. Beijing: University of Chinese Academy of Sciences, 2015.
王晓波. 我国北方草地土壤微生物群落的空间格局及其驱动机制. 北京: 中国科学院大学, 2015.
[28] Xie X H, Liu N, Yang B, et al. Comparison of microbial community in hydrolysis acidification reactor depending on different structure dyes by illumina MiSeq sequencing. International Biodeterioration and Biodegradation, 2016, 111: 14-21.
[29] Pointing S B, Chan Y K, Lacap D C, et al. Highly specialized microbial diversity in hyper-arid polar desert. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(47): 19964-19969.
[30] Fierer N, Leff J W, Adams B J, et al. Cross-biome metagenomic analyses of soil microbial communities and their functional attributes. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(52): 21390-21395.
[31] Lauber C L, Strickland M S, Bradford M A, et al. The influence of soil, properties on the structure of bacterial and fungal communities across land-use types. Soil Biology & Biochemistry, 2008, 40: 2407-2415.
[32] Chu H Y, Fierer N, Lauber C L, et al. Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes. Environment Microbiology, 2010, 12(11): 2998-3006.
[33] Griffiths R I, Thomson B C, James P, et al. The bacterial biogeography of British soils. Environment Microbiology, 2011, 13(6): 1642-1654.