琥珀酸黄杆菌缓解遮阴对多年生黑麦草胁迫的效应

doi:10.11686/cyxb2019391

摘要/Abstract

摘要： 为探究琥珀酸黄杆菌(FS)对遮阴胁迫下多年生黑麦草(LP)的缓解效应,在盆栽试验条件下,研究了不同遮阴胁迫下(0、20%、40%、60%和80%)接种琥珀酸黄杆菌DSM4002(FS)对LP生长和生理特性的影响。结果表明,遮阴胁迫显著抑制LP的生长,而FS能够有效缓解遮阴胁迫对LP的生长抑制。60%和80%遮阴导致多年生黑麦草积累较高的活性氧和丙二醛,FS处理有效地缓解了遮阴对LP幼苗造成的氧化损伤,20%遮阴+FS、40%遮阴+FS、60%遮阴+FS和80%遮阴+FS处理中氧自由基含量分别与单独遮阴处理相比下降4.47%、29.75%、44.57%和49.84%。遮阴胁迫不同程度地改变了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性以及抗坏血酸(AsA)和还原性谷胱甘肽(GSH)含量,接种FS能够进一步提高SOD、CAT、GR活性及AsA和GSH水平,但是接种FS仅能够显著提高40%遮阴胁迫下POD活性和60%遮阴胁迫下APX活性。由此可见,遮阴胁迫下接种FS能够显著提高LP的抗氧化系统活性,增加活性氧的清除能力,减轻遮阴引起的氧化损伤,从而增强LP的耐阴性。

关键词: 琥珀酸黄杆菌, 根围促生菌, 遮阴胁迫, 多年生黑麦草

Abstract: This study aimed to investigate the effects of the plant growth-promoting rhizobacteria Flavobacterium succinicans DSM4002 (FS) on plant growth and physiological characteristics of perennial ryegrass under different shade stress treatments (0, 20%, 40%, 60% and 80%). The combined effects of DSM4002 inoculation and various levels of shade stress on the plant morphology, malondialdehyde (MDA) content, superoxide anion free radical ($O_{2}^{·-}$) levels, antioxidant enzyme activities, and non-enzyme antioxidant contents, were assessed. It was found that shade stress significantly inhibited the growth of perennial ryegrass seedlings while FS treatment alleviated the growth inhibition caused by shade stress. MDA accumulation increased with increasing shade stress. However, FS inoculation significantly reduced MDA and $O_{2}^{·-}$levels under shade stress, compared with shade treatment alone. Treatments of 20% shade+FS, 40% shade+FS, 60% shade+FS, and 80% shade+FS resulted in decreases in $O_{2}^{·-}$ levels of 4.47%, 29.75%, 44.57% and 49.84%, respectively. Additionally, shade stress resulted in a change in antioxidant enzyme activities and non-enzyme antioxidant concentrations, while FS inoculation further improved the activities of superoxide dismutase, catalase, and glutathione reductase, and increased levels of reduced ascorbate and reduced glutathione. Peroxidase and ascorbate peroxidase activities were unchanged by FS. In conclusion, inoculation with FS stimulated the antioxidant defense mechanisms, preventing the accumulation of reactive oxygen species, thus enhancing shade tolerance in perennial ryegrass. The present study indicates that F. succinicans DSM4002 has a great potential to improve plant adaptation to stress.

Key words: Flavobacterium succinicans, rhizosphere bacteria, shading stress, perennial ryegrass

周瀚洋, 孙鹏越, 尉欣荣, 周雨, 张智伟, 高金柱, 赵东豪, 罗艺岚, 呼天明, 付娟娟. 琥珀酸黄杆菌缓解遮阴对多年生黑麦草胁迫的效应[J]. 草业学报, 2020, 29(6): 137-143.

ZHOU Han-yang, SUN Peng-yue, YU Xin-rong, ZHOU Yu, ZHANG Zhi-wei, GAO Jin-zhu, ZHAO Dong-hao, LUO Yi-lan, HU Tian-ming, FU Juan-juan. Protective effects of Flavobacterium succinicans on perennial ryegrass under shade stress[J]. Acta Prataculturae Sinica, 2020, 29(6): 137-143.

参考文献

[1] Zhen C D, Yu M, Xiao H D, et al. Influences of silicon on content of soluble sugars, amino acids in turf-grasses under shading stress. Journal of Huazhong Agricultural University, 2010, 29(3): 317-320.
甄畅迪, 喻敏, 萧洪东, 等. 硅对遮阴处理草坪草可溶性糖和氨基酸含量的影响. 华中农业大学学报, 2010, 29(3): 317-320.
[2] Song X L, Yang H Y, Zeng L Q, et al. Study on the shading impact on plant. Northern Horticulture, 2009, 5: 129-133.
宋晓蕾, 杨红玉, 曾黎琼, 等. 植物遮阴效应的研究进展. 北方园艺学报, 2009, 5: 129-133.
[3] Fan Y, Chen J, Wang Z L, et al. Soybean (Glycine max L. Merr.) seedlings response to shading: Leaf structure, photosynthesis and proteomic analysis. BMC Plant Biology, 2019, 19(1): 34.
[4] Wang S S, Cai J, Liu J P, et al. Effect of soil substrate and shade on the seedling components and biomass allocation of Zoysia japon in the winter. Acta Agrestia Sinica, 2016, 24(6): 1296-1303.
王思思, 蔡捡, 刘金平, 等. 基质和遮阴对结缕草冷季生长幼苗构件及生物量分配的影响. 草地学报, 2016, 24(6): 1296-1303.
[5] Khan A L, Waqas M, Asaf S, et al. Plant growth-promoting endophyte Sphingomonas sp. LK11 alleviates salinity stress in Solanum pimpinellifolium. Environmental and Experimental Botany, 2017, 133: 58-69.
[6] Rizvi A, Khan M S. Biotoxic impact of heavy metals on growth, oxidative stress and morphological changes in root structure of wheat (Triticum aestivum L.) and stress alleviation by Pseudomonas aeruginosa strain CPSB1. Chemosphere, 2017, 185: 942-952.
[7] Naylor D, Coleman-Derr D. Drought stress and root-associated bacterial communities. Frontiers in Plant Science, 2018, 8: 22-23.
[8] Bharti N, Barnawal D, Maji D, et al. Halotolerant PGPRs prevent major shifts in indigenous microbial community structure under salinity stress. Microbial Ecology, 2015, 70: 196-208.
[9] Hu J C, Xue D L, Ma C X, et al. Research advances in plant growth-promoting rhizobacteria and its application prospects. Chinese Journal of Applied Ecology, 2004, 10: 1963-1966.
胡江春, 薛德林, 马成新, 等. 植物根际促生菌(PGPR)的研究与应用前景. 应用生态学报, 2004, 10: 1963-1966.
[10] Zhou C, Ma Z Y, Zhu L, et al. Rhizobacterial strain Bacillus megaterium BOFC15 induces cellular polyamine changes that improve plant growth and drought resistance. International Journal of Molecular Sciences, 2016, 17: 976.
[11] Li L, Li L, Wang X Y, et al. Plant growth-promoting endophyte Piriformospora indica alleviates salinity stress in Medicago truncatula. Plant Physiology and Biochemistry, 2017, 119: 211-223.
[12] Gill S S, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 2010, 48(12): 909-930.
[13] Li H S. Principles and techniques of plant physiological and biochemical experiments (The second edition). Beijing: Higher Education Press, 2006.
李合生. 现代植物生理学(第2版). 北京: 高等教育出版社, 2006.
[14] Elstner E F, Heupel A. Inhibition of nitrite formation from hydroxylammo-iumchloride: A simple assay for superoxide dismutase. Analytical Biochemistry, 1976, 70(2): 616-620.
[15] Beauchamp C, Fridovich I. Superoxide dismutase improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 1971, 44(1): 276-287.
[16] Hammerschmidt R, Nuckles E M, Kuc J. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrchum lagenarium. Physiological and Molecular Plant Pathology, 1982, 20(1): 73-82.
[17] Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiology, 1992, 98(4): 1222-1227.
[18] Foyer C H, Halliwei I B. The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta, 1976, 133: 21-25.
[19] Zhang Y, Xia G H, Ma K, et al. Effects of shade on photosynthetic characteristics and chlorophyll fluorescence of Ardisia violacea. Chinese Journal of Applied Ecology, 2014, 25(7): 1940-1948.
张云, 夏国华, 马凯, 等. 遮阴对堇叶紫金牛光合特性和叶绿素荧光参数的影响. 应用生态学报, 2014, 25(7): 1940-1948.
[20] Pires M V, Almeida A A F, Figueiredo A L, et al. Photosynthetic characteristics of ornamental passion flowers grown under different light intensities. Photosynthetica, 2011, 49: 593-602.
[21] Lü B S, Yan Z W, Tian H W, et al. Local auxin biosynthesis mediates plant growth and development. Trends in Plant Science, 2019, 24(1): 6-9.
[22] Yang Y, Yang X H, Sun Y. Effect on turf characteristics of the Kentucky bluegrass turf under different shading intensities. Acta Agrestia Sinica, 2010, 18(3): 447-451.
杨燕, 杨晓华, 孙彦. 不同遮荫强度对草地早熟禾草坪质量的影响. 草地学报, 2010, 18(3): 447-451.
[23] Xie Y R, Liu Y, Wang H, et al. Phytochrome-interacting factors directly suppress MIR156 expression to enhance shade-avoidance syndrome in Arabidopsis. Nature Communications, 2017, 8: 348.
[24] Hussain S, Iqbal N, Brestic M, et al. Changes in morphology, chlorophyll fluorescence performance and rubisco activity of soybean in response to foliar application of ionic titanium under normal light and shade environment. Science of the Total Environment, 2019, 658: 626-637.
[25] Etesami H,Maheshwari D K. Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicology and Environmental Safety, 2018, 156: 225-246.
[26] Zhang X H, Liu Y H, Liu Q, et al. Nitric oxide is involved in abscisic acid-induced photosynthesis and antioxidant system of tall fescue seedlings response to low-light stress. Environmental and Experimental Botany, 2018, 155: 226-238.
[27] Kumar M, Mishra S, Dixit V, et al. Synergistic effect of Pseudomonas putida and Bacillus amyloliquefaciens ameliorates drought stress in chickpea (Cicer arietinum L.). Plant Signaling & Behavior, 2016, 11(1): e1071004.
[28] Park Y G, Mun B G, Kang S, et al. Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones. PLoS One, 2017, 12(3): e0173203.
[29] Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 2002, 7(9): 405-410.