干旱胁迫下水杨酸和内生真菌对多年生黑麦草的影响

doi:10.11686/cyxb2019103

摘要/Abstract

摘要： 本试验研究了温室盆栽条件下带内生真菌(E+)和不带内生真菌(E-)的多年生黑麦草喷施不同浓度水杨酸(SA)后,持续干旱胁迫15 d和对照水分两种条件下,多年生黑麦草的叶片相对含水量(RLWC)、叶绿素含量(Chl)、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脯氨酸(Pro)和可溶性糖(SS)含量等生理指标的变化,以明确干旱胁迫下SA和内生真菌对多年生黑麦草的影响。结果表明,在干旱胁迫下,感染内生真菌的多年生黑麦草叶片相对含水量、叶绿素含量、POD活性、SS含量显著(P<0.05)高于未感染内生真菌的多年生黑麦草植株;一定浓度的 SA增加了多年生黑麦草叶片相对含水量、叶绿素含量、POD活性、SOD活性、Pro和SS含量,降低了MDA含量,且最适水杨酸浓度为0.25和0.50 mmol·L^-1;内生真菌与SA互作显著(P<0.05)提高了多年生黑麦草SOD活性、POD活性和叶绿素含量。该结果说明内生真菌与SA都能提高多年生黑麦草的抗旱性,即内生真菌和SA通过提高寄主的渗透调节和活性氧清除系统进而提高了寄主的抗旱性。

关键词: 多年生黑麦草, 内生真菌, 水杨酸, 干旱胁迫

Abstract: This greenhouse experiment studied the effects of different concentrations of salicylic acid (SA) on endophyte-infected (E+) and endophyte-free (E-) perennial ryegrass (Lolium perenne), under continuous drought stress for 15 days or well watered (Control). Measurements included leaf relative leaf water content, chlorophyll content, peroxidase (POD) activity, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, proline (Pro) and soluble sugar (SS) of the perennial ryegrass. Changes in physiological indices such as SS content were examined to determine the effects of SA and endophytic fungi on perennial ryegrass under drought stress. It was found that the relative water content, chlorophyll content, POD activity and SS content of E+ were significantly higher (P<0.05) than those of E- L. perenne plants under drought stress. The addition of exogenous SA increased the relative water content, chlorophyll content, POD and SOD activities, and Pro and SS contents, while decreasing MDA content. The present study showed that exogenous SA solutions of 0.25 and 0.50 mmol·L^-1 were best for enhancement of the drought tolerance of L. perenne. There was a significant (P<0.05) interaction effect between Epichloё endophyte and SA together on SOD activity, POD activity and chlorophyll content of perennial ryegrass. In summary, Epichloё endophyte and SA both improved the drought resistance of perennial ryegrass. Specifically, Epichloё endophyte and SA promoted osmotic adjustment and activity of oxygen radical scavenging system, and thereby increased the drought resistance of the host plant.

Key words: perennial ryegrass, Epichloё, salicylic acid, drought stress

马碧花, 蔺伟虎, 高敏, 王兴迪, 田沛. 干旱胁迫下水杨酸和内生真菌对多年生黑麦草的影响[J]. 草业学报, 2020, 29(1): 135-144.

MA Bi-hua, LIN Wei-hu, GAO Min, WANG Xing-di, TIAN Pei. Effects of salicylic acid and Epichloё on perennial ryegrass (Lolium perenne) under drought stress[J]. Acta Prataculturae Sinica, 2020, 29(1): 135-144.

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