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草业学报 ›› 2022, Vol. 31 ›› Issue (6): 150-162.DOI: 10.11686/cyxb2021130

• 研究论文 • 上一篇    

鸭茅响应水淹胁迫的miRNA差异表达分析

杨兴云1(), 乔丹丹1, 张雅洁1, 王少青2, 任俊才1, 李明阳1, 屈明好1, 尚盼盼1, 杨成1, 黄琳凯3,4(), 曾兵1,2()   

  1. 1.西南大学动物科学技术学院,重庆 402460
    2.草食动物科学重庆市重点实验室,重庆 400715
    3.福建傲农生物科技集团股份有限公司,福建 厦门 361111
    4.四川农业大学草业科技学院,四川 成都 611130
  • 收稿日期:2021-04-07 修回日期:2021-07-07 出版日期:2022-06-20 发布日期:2022-05-11
  • 通讯作者: 黄琳凯,曾兵
  • 作者简介:E-mail: huanglinkai@siau.edu.cn
    E-mail: zbin78@163.com
    杨兴云(1995-),男,云南曲靖人,在读硕士。E-mail: yxingyun12@163.com
  • 基金资助:
    中央高校基本科研业务费项目荣昌专项(XDJK2020RC001);贵州省科技计划项目(黔科合支撑[2021]一般226)和重庆市现代山地特色高效农业产业技术体系(草食牲畜)2022(12)资助

A differential gene expression analysis of miRNA in Dactylis glomerata in response to flooding stress

Xing-yun YANG1(), Dan-dan QIAO1, Ya-jie ZHANG1, Shao-qing WANG2, Jun-cai REN1, Ming-yang LI1, Ming-hao QU1, Pan-pan SHANG1, Cheng YANG1, Lin-kai HUANG3,4(), Bing ZENG1,2()   

  1. 1.College of Animal Science and Technology,Southwest University,Chongqing 402460,China
    2.Chongqing Key Laboratory of Herbivore Science,Chongqing 400715,China
    3.Fujian Aonong Biotechnology Group Co. ,Ltd. ,Xiamen 361111,China
    4.College of Grassland Science and Technology,Sichuan Agricultural University,Chengdu 611130,China
  • Received:2021-04-07 Revised:2021-07-07 Online:2022-06-20 Published:2022-05-11
  • Contact: Lin-kai HUANG,Bing ZENG

摘要:

水淹胁迫是限制我国西南地区鸭茅产量和品质提升的主要环境因子,已经成为一种不容忽视的非生物胁迫。鉴定鸭茅耐涝相关的功能基因,并探究其调控机制是鸭茅种质创新,提高鸭茅耐涝能力的必要途径。以鸭茅耐涝品种“滇北”为试验材料,分别经水淹胁迫处理0、8和24 h后,利用Illumina Hiseq测序平台对鸭茅叶片进行小RNA测序。结果表明,在水淹胁迫处理下共鉴定得到208个差异表达基因(DEGs),经过筛选后有38个基因上调表达,34个基因下调表达,共占差异表达基因的34.62%。“滇北”鸭茅在水淹胁迫下差异表达基因主要属于miR166、miR167、miR159、miR396和miR156这5个miRNA基因家族。基于对差异miRNA进行靶基因预测及靶基因的GO和KEGG功能分析,发现这些靶基因主要参与细胞生理过程、代谢过程、IL-17信号通路、Th17细胞分化等植物逆境响应过程,为进一步揭示鸭茅在水淹胁迫下的分子调控机制提供了研究线索。

关键词: 鸭茅, 水淹胁迫, 耐涝, miRNA, 差异表达

Abstract:

Waterlogging stress is the main environmental factor that restricts the improvement of yield and quality of orchard grass (Dactylis glomerata) in Southwest China, and it has become a category of abiotic stress that can’t be ignored, from a research perspective. This research aimed to identify functional genes related to waterlogging tolerance of orchard grass, and explore its regulation mechanisms, as a means of improving the waterlogging tolerance of this grass. In this research, the waterlogging tolerant variety “Dian bei” was studied. After being subjected to flooding stress for 0, 8 and 24 h, RNA was extracted from orchard grass leaves and sequencing was carried out using an Illumina Hiseq sequencing platform. A total of 208 differentially expressed genes were identified under flooding stress, 38 genes were up-regulated and 34 genes were down-regulated, accounting for 34.62% of the differentially expressed genes (DEGs). The genes identified as differentially in “Dian bei” orchard grass under flooding stress mainly belong to five miRNA gene families: miR166, miR167, miR159, miR396 and miR156. Based on the predicted function of target genes of differentially expressed miRNA and functional analysis using GO(gene ontology) and KEGG (kyoto encyclopedia of genes and genomes) of target genes, it was determined that the identified DEGs were mainly involved in plant stress response processes, such as cellular processes, metabolic processes, the IL-17 signaling pathway, Th17 cell differentiation, and others. These results provide initial direction for further study of the molecular regulation and response mechanisms of orchard grass under flooding stress.

Key words: Dactylis glomerata, flooding stress, waterlogging tolerance, miRNA, differential expression