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草业学报 ›› 2021, Vol. 30 ›› Issue (10): 116-124.DOI: 10.11686/cyxb2020368

• 研究论文 • 上一篇    

盐处理下旱生植物沙芥蛋白激酶相关基因的差异表达分析

汪芳珍(), 杨成行(), 何子华, 林子茹, 曾浩源, 马清()   

  1. 兰州大学农业农村部草牧业创新重点实验室,兰州大学草地农业科技学院,甘肃 兰州 730020
  • 收稿日期:2020-07-29 修回日期:2020-10-19 出版日期:2021-09-16 发布日期:2021-09-16
  • 通讯作者: 马清
  • 作者简介:Corresponding author. E-mail: maq@lzu.edu.cn
    汪芳珍(1994-),女,甘肃天水人,在读硕士。E-mail: wangfzh18@lzu.edu.cn
    杨成行(1994-),男,贵州桐梓人,硕士。E-mail: 13557012949@163.com第一联系人:共同第一作者These authors contributed equally to this work.
  • 基金资助:
    国家重点研发计划(2017YFC0504804)

Analysis of differentially expressed protein kinase related genes in the xerophyte Pugionium cornutum under salt treatment

Fang-zhen WANG(), Cheng-hang YANG(), Zi-hua HE, Zi-ru LIN, Hao-yuan ZENG, Qing MA()   

  1. Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China
  • Received:2020-07-29 Revised:2020-10-19 Online:2021-09-16 Published:2021-09-16
  • Contact: Qing MA

摘要:

旱生植物沙芥具有极强的耐盐能力,对其耐盐相关分子基础的研究将为农作物和牧草抗逆性遗传改良提供重要的基因资源。前期研究已采用转录组学研究方法分析了盐胁迫下沙芥功能基因的差异表达情况,并筛选了一批与沙芥耐盐性相关的重要候选功能基因,而盐胁迫下沙芥体内调控基因的表达变化情况未见报道。为进一步揭示沙芥耐盐分子机制,本研究利用已获得的50 mmol·L-1 NaCl处理6和24 h后沙芥根和叶组织的转录组数据,分析了盐胁迫下沙芥体内蛋白激酶相关基因的差异表达情况。结果表明,50 mmol·L-1 NaCl处理下沙芥体内大量蛋白激酶相关基因表达发生显著变化。其中,根和地上部中大量富亮氨酸重复类受体蛋白激酶(LRR-RLKs)编码基因的表达在50 mmol·L-1 NaCl处理6和24 h后均显著上调;50 mmol·L-1 NaCl短期处理(6 h)后,根和地上部中众多促分裂原活化蛋白激酶级联途径(MAPK/MAPKK/MAPKKK)相关基因的表达被显著诱导,而一些乙烯信号转导途径中重要负调控因子CTR1编码基因在对照处理下均有表达,但在50 mmol·L-1 NaCl处理6 h后的根中均不表达。上述结果表明,LRR-RLK家族蛋白在沙芥适应盐胁迫过程中可能发挥着重要的调控作用,MAPK/MAPKK/MAPKKK可能参与调控沙芥对短期盐胁迫的响应,CTR1可能在沙芥根系响应盐胁迫过程中发挥负调控功能。

关键词: 沙芥, 盐胁迫, 蛋白激酶, 差异表达基因

Abstract:

The xerophyte Pugionium cornutum possesses strong salt tolerance. Study of the molecular basis of its salt tolerance will provide important genetic resources for the genetic improvement of stress tolerance in crops and forages. In previous studies, by using transcriptomic analysis, the expression pattern of important functional genes in P. cornutum under salt stress has been analyzed, and a number of candidate functional genes related to salt-tolerance of P. cornutum were identified; however, the expression of regulatory genes responding to salt stress in P. cornutum have not been reported. In order to further explore the molecular mechanism concerning salt tolerance of P. cornutum, in this study, the expression pattern of protein kinase related genes in P. cornutum under salt treatment was analyzed, based on the transcriptomic data of root and shoot tissues of P. cornutum exposed to 50 mmol·L-1 NaCl for 6 and 24 h. The results showed that there were significant changes in the expression of a large number of protein kinase related genes in P. cornutum under 50 mmol·L-1 NaCl. Among them, the expression of numerous genes encoding leucine-rich repeat receptor protein kinases (LRR-RLKs) were significantly up-regulated in both the root and shoot of P. cornutum after treatment with 50 mmol·L-1 NaCl for both 6 and 24 h; many genes related to mitogen-activated protein kinase cascade pathways (MAPK/MAPKK/MAPKKK) were significantly up-regulated under short-term salt stress (50 mmol·L-1 NaCl for 6 h). Some genes encoding CTR1, an important negative regulator of the ethylene signal transduction pathway, were expressed in roots of P. cornutum under control condition but not expressed in roots under 50 mmol·L-1 NaCl treatment for 6 h. These results indicate that LRR-RLK proteins may play an important regulatory role in the adaptation of P. cornutum to salt stress, MAPK/MAPKK/MAPKKK may be involved in regulating the response of P. cornutum to short-term salt stress, and CTR1 might function as a negative regulator in the response of P. cornutum to salt stress.

Key words: Pugionium cornutum, salt stress, protein kinase, differentially expressed genes (DEGs)