草业学报 ›› 2023, Vol. 32 ›› Issue (8): 141-151.DOI: 10.11686/cyxb2022252
• 研究论文 • 上一篇
王宝强1,2,3(), 马文静1(), 王贤1,4, 朱晓林1,4, 赵颖1,2,3, 魏小红1,2,3,4()
收稿日期:
2022-06-16
修回日期:
2022-10-19
出版日期:
2023-08-20
发布日期:
2023-06-16
通讯作者:
魏小红
作者简介:
E-mail: weixh@gsau.edu.cn基金资助:
Bao-qiang WANG1,2,3(), Wen-jing MA1(), Xian WANG1,4, Xiao-lin ZHU1,4, Ying ZHAO1,2,3, Xiao-hong WEI1,2,3,4()
Received:
2022-06-16
Revised:
2022-10-19
Online:
2023-08-20
Published:
2023-06-16
Contact:
Xiao-hong WEI
摘要:
本研究以盆栽紫花苜蓿为试验材料,在聚乙二醇(PEG-6000)模拟干旱的条件下,分别以硝普钠、c-PTIO(carboxy-PTIO)为NO供体和清除剂进行叶面喷施,通过研究紫花苜蓿幼苗叶片、根系中次生代谢产物及关键代谢酶活性的变化,探讨干旱胁迫对次生代谢产物的影响及NO介导下紫花苜蓿耐旱机制的响应。结果表明,干旱胁迫使紫花苜蓿叶片及根系中总酚、类黄酮、木质素和花青素的含量呈先升高后降低的变化趋势,同时增强了苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性。外源NO的施加则会促进这种趋势的进行,使总酚含量提高33.08%,在胁迫后期仍使酚类物质保持较高的代谢水平,改善胁迫所导致的损伤状况。而NO抑制剂 c-PTIO则会不同程度地抑制紫花苜蓿幼苗中酚类物质的合成及其代谢关键酶的活性。因此,外源NO的喷施使紫花苜蓿中次生代谢物的积累与抗氧化酶活性维持在一个高于仅干旱胁迫的水平,更大限度地降低干旱对紫花苜蓿造成的危害,提高对干旱的耐受性。本研究进一步扩充了紫花苜蓿抗旱手段的工具箱,对紫花苜蓿的抗旱具有重要的意义。
王宝强, 马文静, 王贤, 朱晓林, 赵颖, 魏小红. 一氧化氮对干旱胁迫下紫花苜蓿幼苗次生代谢产物的影响[J]. 草业学报, 2023, 32(8): 141-151.
Bao-qiang WANG, Wen-jing MA, Xian WANG, Xiao-lin ZHU, Ying ZHAO, Xiao-hong WEI. Nitric oxide regulation of secondary metabolite accumulation in Medicago sativa seedlings under drought stress[J]. Acta Prataculturae Sinica, 2023, 32(8): 141-151.
图1 NO对干旱胁迫下紫花苜蓿幼苗叶片、根系总酚含量变化的影响图中数据为均值±标准误差,同一处理天数不同字母表示不同处理间在0.05水平存在显著性的差异(P<0.05),下同。Data are presented as the means±standard error. The different letters in each treatment time indicate significant differences in different treatments at 0.05 levels (P<0.05),the same below.
Fig.1 Effects of nitric oxide on total phenols content of alfalfa seedings leaf and root under drought stress
图5 NO对干旱胁迫下紫花苜蓿幼苗叶片、根系苯丙氨酸解氨酶(PAL)活性变化的影响
Fig.5 Effects of nitric oxide on phenylalanine ammonialyase (PAL) activity of alfalfa seedings leaf and root under drought stress
图6 NO对干旱胁迫下紫花苜蓿幼苗叶片、根系多酚氧化酶(PPO)活性变化的影响
Fig.6 Effects of nitric oxide on polyphenol oxidase (PPO) activity of alfalfa seedings leaf and root under drought stress
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