44个紫花苜蓿品种的酸铝适应性与耐受性评价

doi:10.11686/cyxb2022097

摘要/Abstract

摘要：

为了解紫花苜蓿在贵州地区的适应性及耐酸铝胁迫机理，以44份紫花苜蓿品种为研究对象，研究紫花苜蓿处于酸铝胁迫下的生理变化，并揭示其生理变化与耐酸铝胁迫间的关系。利用基因与环境互作模型对两个地点1年的紫花苜蓿进行产量分析，筛选出阿尔冈金、新疆大叶苜蓿、Trifecta、Vernal和中牧1号苜蓿5个耐酸铝强适应品种。利用敏感型UC-1465和耐受型阿尔冈金进行酸铝胁迫试验。结果表明：相同处理下，耐受型紫花苜蓿的电导率、相对铝含量、死亡率显著低于敏感型；紫花苜蓿对酸铝胁迫的响应主要通过柠檬酸、苹果酸、乙酸、酒石酸、反丁烯二酸和草酸的显著（P<0.05）增加来体现，其中苹果酸的合成和分泌增多可能是其耐酸铝胁迫的重要原因。

关键词: 紫花苜蓿, 农艺性状, 酸铝胁迫, 有机酸, 适应性, 耐受性评价

Abstract:

The aims of this study were to explore the range of adaptability of Medicago sativa cultivars in Guizhou to acid-aluminum conditions， and to determine which physiological factors are related to tolerance of these conditions. We selected 44 alfalfa varieties for a one-year field experiment. A genotype×environment interaction model was used to analyze the yield of alfalfa at two locations in one year， and five acid-tolerant and aluminum-adaptive varieties were identified： Algonquin， M. sativa cv. xinjiangdaye， Trifecta， Vernal and Medicago varia cv. Zhongmu No.1. Aluminum-acid stress experiments were carried out using the sensitive variety UC-1465 and the tolerant variety Algonquin. It was found that， under the same treatment， the electrical conductivity， relative aluminum content， and mortality rates were significantly lower in the tolerant alfalfa cultivar than in the sensitive alfalfa cultivar. The response of alfalfa plants to acid-aluminum stress was mainly reflected by significant （P<0.05） increases in citric acid， malic acid， acetic acid， tartaric acid， fumaric acid， and oxalic acid contents. The increased synthesis and secretion of malic acid may be an important factor in resistance to acid and aluminum stress.

Key words: Medicago sativa, agronomic traits, acid-aluminum stress, organic acid, adaptability, evaluation of tolerance

田政, 杨正禹, 陆忠杰, 罗奔, 张茂, 董瑞. 44个紫花苜蓿品种的酸铝适应性与耐受性评价[J]. 草业学报, 2023, 32(3): 142-151.

Zheng TIAN, Zheng-yu YANG, Zhong-jie LU, Ben LUO, Mao ZHANG, Rui DONG. Acid-aluminum adaptability and tolerance evaluation of 44 alfalfa cultivars[J]. Acta Prataculturae Sinica, 2023, 32(3): 142-151.

图/表 8

参考文献 37

1	Von Uexküll H R， Mutert E. Global extent， development and economic impact of acid soils. Plant and Soil， 1995， 171（1）： 1-15.
2	Huang C Q， Chen Z， Liu G D， et al. The physiological response of the warm-season turfgrass Cynodon dactylon accessions to acidity and aluminum stress. Acta Agrestia Sinica， 2017， 25（4）： 796-802.
	黄春琼，陈振，刘国道，等. 暖季型草坪草狗牙根对酸铝胁迫的生理响应. 草地学报， 2017， 25（4）： 796-802.
3	Horst W J， Wang Y， Eticha D. The role of the root apoplast in aluminium-induced inhibition of root elongation and in aluminium resistance of plants： A review. Annals of Botany， 2010， 106（1）： 185-197.
4	Kinraide T B. Identity of the rhizotoxic aluminium species//Plant-soil interactions at low pH. Dordrecht： Springer Netherlands， 1991： 717-728.
5	Čiamporová M. Diverse responses of root cell structure to aluminium stress. Plant and Soil， 2000， 226（1）： 113-116.
6	Čiamporová M. Morphological and structural responses of plant roots to aluminium at organ， tissue， and cellular levels. Biologia Plantarum， 2002， 45（2）： 161-171.
7	Zhang X H， Zhang Z， Xu N， et al. Effects of growth traits and grass yield of alfalfa in different sowing dates. Chinese Agricultural Science Bulletin， 2011， 27（14）： 27-30.
	张晓华，张众，徐娜，等. 不同播期对紫花苜蓿生长性状及草产量的影响. 中国农学通报， 2011， 27（14）： 27-30.
8	Cao H， Ma S F， Han Y， et al. Introduction and selection of 5 alfalfa cultivars in Longdong Region. Pratacultural Science， 2014， 31（9）： 1761-1766.
	曹宏，马生发，韩雍，等. 5个紫花苜蓿品种在陇东地区的引进筛选试验. 草业科学， 2014， 31（9）： 1761-1766.
9	Zhang B Y. Function and expression regulation aluminum-responsive genes MsMATE and MsSTOP1 in Medicago sativa. Chongqing： Chongqing University， 2017.
	张宝云. 紫花苜蓿铝胁迫响应基因MsMATE与MsSTOP1的功能与表达调控研究. 重庆：重庆大学， 2017.
10	Ren X Y. Effects of aluminum toxicity on the growth and IAA distribution in alfalfa. Shanghai： Shanghai Jiao Tong University， 2013.
	任晓燕. 铝毒对紫花苜蓿幼苗生长和IAA分布的影响. 上海：上海交通大学， 2013.
11	Jiang N， Tang M， Han B， et al. Effects of aluminum stress on different germplasm materials of alfalfa and its comprehensive evaluation. Acta Agrestia Sinica， 2019， 27（3）： 620-627.
	姜娜，唐敏，韩博，等. 铝胁迫对紫花苜蓿不同种质材料的影响及综合评价. 草地学报， 2019， 27（3）： 620-627.
12	Ma J F， Ryan P R， Delhaize E. Aluminium tolerance in plants and the complexing role of organic acids. Trends in Plant Science， 2001， 6（6）： 273-278.
13	Barone P， Rosellini D， LaFayette P， et al. Bacterial citrate synthase expression and soil aluminum tolerance in transgenic alfalfa. Plant Cell Reports， 2008， 27（5）： 893-901.
14	Sun G， Zhu H， Wen S， et al. Citrate synthesis and exudation confer Al resistance in alfalfa （Medicago sativa L.）. Plant and Soil， 2020， 449（1）： 319-329.
15	Chen A K， Han R H， Li D Y， et al. A comparison of two methods for electrical conductivity about plant leaves. Journal of Guangdong Education Institute， 2010， 30（5）： 88-91.
	陈爱葵，韩瑞宏，李东洋，等. 植物叶片相对电导率测定方法比较研究. 广东教育学院学报， 2010， 30（5）： 88-91.
16	Wang Y M， Zhang L L， Zhang X W， et al. Comparative study on three pretreatment methods for atomic absorptive spectrophotometry determination of metal elements in Lycium barbarum. Spectroscopy and Spectral Analysis， 2017， 37（3）： 914-918.
	王益民，张莉莉，张筱文，等. 原子吸收法测定枸杞子矿物质元素的三种前处理方法比较. 光谱学与光谱分析， 2017， 37（3）： 914-918.
17	Li W Y， Gu W C. Study on phenotypic diversity of natural population in Quercus mongolica. Scientia Silvae Sinicae， 2005（1）： 49-56.
	李文英，顾万春. 蒙古栎天然群体表型多样性研究. 林业科学， 2005（1）： 49-56.
18	Liu M X. The primary study of the production performance and ecological adaptation of twelve introduced alfalfa varieties in the heat-humid areas of southwest in Sichuan Province. Ya’an： Sichuan Agricultural University， 2005.
	刘明秀. 12个紫花苜蓿品种在川西南湿热区的生产性能及生态适应性初步研究. 雅安：四川农业大学， 2005.
19	Mo B T， Zhang J B， Zhang W， et al. Adaptability of 48 introduced Medicago sativa varieties in southern of Guizhou Province. Guizhou Agricultural Sciences， 2010， 38（9）： 155-159.
	莫本田，张建波，张文，等. 48个紫花苜蓿品种在贵州南部的适应性研究. 贵州农业科学， 2010， 38（9）： 155-159.
20	Liu H Y. The evaluation of production performance and adaptability to 49 kinds of alfalfa in Xilinhot. Hohhot： Inner Mongolia University， 2017.
	柳海鹰. 锡林浩特地区49个苜蓿品种的适应性及生产性能评价. 呼和浩特：内蒙古大学， 2017.
21	Wang Y Q， Wu X M， Liu J N， et al. The evaluation of salt tolerance at seeding stage alfalfa for introduced. Prataculture and Animal Husbandry， 2009， 29（3）： 22-28.
	王运琦，吴欣明，刘建宁，等. 引进紫花苜蓿耐盐性的评价. 草业与畜牧， 2009， 29（3）： 22-28.
22	Tamás L， Huttová J， Mistrík I， et al. Aluminium-induced drought and oxidative stress in barley roots. Journal of Plant Physiology， 2006， 163（7）： 781-784.
23	Silva I R， Smyth T J， Raper C D， et al. Differential aluminum tolerance in soybean： An evaluation of the role of organic acids. Physiologia Plantarum， 2001， 112（2）： 200-210.
24	Fan K Z， Zhu M Y， Chen L， et al. Comparison of four alfalfa varieties’ several physiological items change in reagents of the acid， alkali and salt. Guihaia， 2012， 32（4）： 516-521.
	范可章，朱茂英，陈灵，等. 酸、碱、盐胁迫下4种紫花苜蓿几项生理指标变化的比较研究. 广西植物， 2012， 32（4）： 516-521.
25	Chen T X. Study on salt tolerance at different growth periods in fall dormancy standard varieties of alfalfa （Medicago sativa）. Beijing： Beijing Forestry University， 2009.
	陈托兄. 不同生育时期紫花苜蓿秋眠型标准品种耐盐机制研究. 北京：北京林业大学， 2009.
26	Liao H， Wan H， Shaff J， et al. Phosphorus and aluminum interactions in soybean in relation to aluminum tolerance. Exudation of specific organic acids from different regions of the intact root system. Plant Physiology， 2006， 141（2）： 674-684.
27	Zhao K， Zhou B H， Ma W Z， et al. The influence of different environmental stresses on root-exuded organic acids： A review. Soils， 2016， 48（2）： 235-240.
	赵宽，周葆华，马万征，等. 不同环境胁迫对根系分泌有机酸的影响研究进展. 土壤， 2016， 48（2）： 235-240.
28	Delhaize E， Craig S， Beaton C D， et al. Aluminum tolerance in wheat （Triticum aestivum L.）（I. Uptake and distribution of aluminum in root apices）. Plant Physiology， 1993， 103（3）： 685-693.
29	Hoekenga O A， Vision T J， Shaff J E， et al. Identification and characterization of aluminum tolerance loci in Arabidopsis （Landsberg erecta× Columbia） by quantitative trait locus mapping. A physiologically simple but genetically complex trait. Plant Physiology， 2003， 132（2）： 936-948.
30	Miyasaka S C， Buta J G， Howell R K， et al. Mechanism of aluminum tolerance in snapbeans： Root exudation of citric acid. Plant Physiology， 1991， 96（3）： 737-743.
31	Ling G Z， Shi B F， Huang Y L， et al. Secretion of organic acid anions and potassium from root apices under Al stress in Secale cereale L. Plant Nutrition and Fertilizer Science， 2010， 16（4）： 893-898.
32	Yang L T， Jiang H X， Tang N， et al. Mechanisms of aluminum-tolerance in two species of citrus： Secretion of organic acid anions and immobilization of aluminum by phosphorus in roots. Plant Science， 2011， 180（3）： 521-530.
33	Takashi I， Tsuyoshi O， Li D H， et al. Effect of aluminum on metabolism of organic acids and chemical forms of aluminum in root tips of Eucalyptus camaldulensis Dehnh. Phytochemistry， 2013， 96： 142-147.
34	Wu X， Li R， Shi J， et al. Brassica oleracea MATE encodes a citrate transporter and enhances aluminum tolerance in Arabidopsis thaliana. Plant and Cell Physiology， 2014， 55（8）： 1426-1436.
35	Dai H， Zhao J， Ahmed I M， et al. Differences in physiological features associated with aluminum tolerance in Tibetan wild and cultivated barleys. Plant Physiology and Biochemistry， 2014， 75： 36-44.
36	Liu J， Luo X， Shaff J， et al. A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. The Plant Journal， 2012， 71（2）： 327-337.
37	Yang T Y， Qi Y P， Huang H Y， et al. Interactive effects of pH and aluminum on the secretion of organic acid anions by roots and related metabolic factors in Citrus sinensis roots and leaves. Environmental Pollution， 2020， 262： 114303.

编号 Serial number	品种名 Variety name	产地 Country	编号 Serial number	品种名 Variety name	产地 Country
1	Abi 700	美国United States	23	WL363HQ	美国United States
2	Arc	美国United States	24	东苜1号Dongmu No. 1	中国China
3	Archer	美国United States	25	甘农3号Gannong No. 3	中国China
4	Boja	波兰Poland	26	甘农4号Gannong No. 4	中国China
5	CUF 101	美国United States	27	甘农5号Gannong No. 5	中国China
6	德宝 Derby	英国United Kingdom	28	甘农6号Gannong No. 6	中国China
7	Hunter River	墨西哥Mexico	29	甘农7号Gannong No. 7	中国China
8	Aurora	危地马拉Guatemala	30	公农2号Gongnong No. 2	中国China
9	Maverick	美国United States	31	皇冠Phabulous	美国United States
10	Orca	法国France	32	陇东Longdong	中国China
11	Ranger	美国United States	33	陇中Longzhong	中国China
12	三得利 Sanditi	法国France	34	天水Tianshui	中国China
13	Saranac AR	美国United States	35	无棣Wudi	中国China
14	Sutter	美国United States	36	新疆大叶Xinjiang daye	中国China
15	Trifecta	奥地利Austria	37	新牧2号Xinmu No. 2	中国China
16	UC-1465	美国United States	38	中兰1号Zhonglan No. 1	中国China
17	UC-1887	美国United States	39	中苜1号Zhongmu No. 1	中国China
18	Vernal	奥地利Austria	40	中牧1号Zhongmu No. 1	中国China
19	WL168HQ	美国United States	41	甘农1号Gannong No. 1	中国China
20	WL319HQ	美国United States	42	甘农2号Gannong No. 2	中国China
21	WL343HQ	美国United States	43	图牧1号Tumu No. 1	中国China
22	WL354HQ	美国United States	44	阿尔冈金Algonquin	中国China

编号 Serial number	品种名 Variety name	产地 Country	编号 Serial number	品种名 Variety name	产地 Country
1	Abi 700	美国United States	23	WL363HQ	美国United States
2	Arc	美国United States	24	东苜1号Dongmu No. 1	中国China
3	Archer	美国United States	25	甘农3号Gannong No. 3	中国China
4	Boja	波兰Poland	26	甘农4号Gannong No. 4	中国China
5	CUF 101	美国United States	27	甘农5号Gannong No. 5	中国China
6	德宝 Derby	英国United Kingdom	28	甘农6号Gannong No. 6	中国China
7	Hunter River	墨西哥Mexico	29	甘农7号Gannong No. 7	中国China
8	Aurora	危地马拉Guatemala	30	公农2号Gongnong No. 2	中国China
9	Maverick	美国United States	31	皇冠Phabulous	美国United States
10	Orca	法国France	32	陇东Longdong	中国China
11	Ranger	美国United States	33	陇中Longzhong	中国China
12	三得利 Sanditi	法国France	34	天水Tianshui	中国China
13	Saranac AR	美国United States	35	无棣Wudi	中国China
14	Sutter	美国United States	36	新疆大叶Xinjiang daye	中国China
15	Trifecta	奥地利Austria	37	新牧2号Xinmu No. 2	中国China
16	UC-1465	美国United States	38	中兰1号Zhonglan No. 1	中国China
17	UC-1887	美国United States	39	中苜1号Zhongmu No. 1	中国China
18	Vernal	奥地利Austria	40	中牧1号Zhongmu No. 1	中国China
19	WL168HQ	美国United States	41	甘农1号Gannong No. 1	中国China
20	WL319HQ	美国United States	42	甘农2号Gannong No. 2	中国China
21	WL343HQ	美国United States	43	图牧1号Tumu No. 1	中国China
22	WL354HQ	美国United States	44	阿尔冈金Algonquin	中国China

品种Germplasm	贵阳Guiyang	石阡Shiqian	品种Germplasm	贵阳Guiyang	石阡Shiqian
Abi 700	320.01±95.82bcdefg	255.80±64.53cdefg	WL363HQ	653.38±564.43a	250.79±81.16cdefg
Arc	302.47±102.33bcdefg	242.60±79.44cdefg	东苜1号Dongmu No. 1	202.40±50.38fg	171.19±37.62fg
Archer	497.17±190.25abcde	244.79±13.49cdefg	甘农3号Gannong No. 3	358.52±154.96bcdefg	297.88±91.18bcdefg
Boja	542.40±119.56abc	310.34±43.68bcdefg	甘农4号Gannong No. 4	312.82±106.19bcdefg	318.14±130.18bcdef
CUF 101	323.86±135.61bcdefg	162.25±27.74fg	甘农5号Gannong No. 5	213.85±66.25efg	215.77±109.70defg
德宝Derby	312.48±131.80bcdefg	248.92±37.84cdefg	甘农6号Gannong No. 6	200.54±35.58fg	217.83±53.13defg
Hunter River	316.43±140.97bcdefg	285.95±139.49bcdefg	甘农7号Gannong No. 7	333.12±224.15bcdefg	224.5±25.81cdefg
Aurora	363.51±158.21bcdefg	229.98±119.94cdefg	公农2号Gongnong No. 2	256.21±72.78cdefg	305.78±166.23bcdefg
Maverick	197.17±75.65fg	197.84±103.63defg	皇冠Phabulous	360.73±78.59bcdefg	341.11±105.01abcde
Orca	153.22±44.00fg	240.32±21.13cdefg	陇东Longdong	173.73±45.48fg	168.28±89.88fg
Ranger	506.51±79.07abcd	240.49±13.29cdefg	陇中Longzhong	383.56±133.14abcdef	161.55±34.42fg
三得利Sanditi	190.76±10.46fg	265.27±45.46cdefg	天水Tianshui	259.03±106.22cdefg	224.34±52.02cdefg
Saranac AR	283.33±63.41bcdefg	188.23±9.01efg	无棣Wudi	236.63±79.72defg	237.59±70.70cdefg
Sutter	340.65±88.37bcdefg	312.95±99.71bcdef	新疆大叶Xinjiang daye	424.21±206.9abcdef	378.21±60.32abc
Trifecta	522.56±45.09abcd	429.94±74.83ab	新牧2号Xinmu No. 2	329.43±154.57bcdefg	256.83±41.31cdefg
UC-1465	84.31±53.91g	150.97±57.17g	中兰1号Zhonglan No. 1	192.54±27.31fg	268.15±97.20cdefg
UC-1887	279.29±74.21bcdefg	201.96±41.10defg	中苜1号Zhongmu No. 1	240.05±66.29defg	167.90±52.59fg
Vernal	418.49±147.06abcdef	350.40±31.95abcd	中牧1号Zhongmu No. 1	249.72±166.26defg	351.74±31.64abcd
WL168HQ	298.74±156.44bcdefg	195.23±67.18defg	甘农1号Gannong No. 1	270.86±228.12cdefg	295.00±159.21bcdefg
WL319HQ	348.54±98.86bcdefg	222.56±31.19cdefg	甘农2号Gannong No. 2	199.08±18.78fg	235.80±26.59cdefg
WL343HQ	393.03±59.76abcdef	221.61±109.10cdefg	图牧1号Tumu No. 1	350.38±128.24bcdefg	260.49±52.36cdefg
WL354HQ	544.17±76.47abc	306.68±18.89bcdefg	阿尔冈金Algonquin	564.15±145.08ab	464.91±123.14a

品种Germplasm	贵阳Guiyang	石阡Shiqian	品种Germplasm	贵阳Guiyang	石阡Shiqian
Abi 700	320.01±95.82bcdefg	255.80±64.53cdefg	WL363HQ	653.38±564.43a	250.79±81.16cdefg
Arc	302.47±102.33bcdefg	242.60±79.44cdefg	东苜1号Dongmu No. 1	202.40±50.38fg	171.19±37.62fg
Archer	497.17±190.25abcde	244.79±13.49cdefg	甘农3号Gannong No. 3	358.52±154.96bcdefg	297.88±91.18bcdefg
Boja	542.40±119.56abc	310.34±43.68bcdefg	甘农4号Gannong No. 4	312.82±106.19bcdefg	318.14±130.18bcdef
CUF 101	323.86±135.61bcdefg	162.25±27.74fg	甘农5号Gannong No. 5	213.85±66.25efg	215.77±109.70defg
德宝Derby	312.48±131.80bcdefg	248.92±37.84cdefg	甘农6号Gannong No. 6	200.54±35.58fg	217.83±53.13defg
Hunter River	316.43±140.97bcdefg	285.95±139.49bcdefg	甘农7号Gannong No. 7	333.12±224.15bcdefg	224.5±25.81cdefg
Aurora	363.51±158.21bcdefg	229.98±119.94cdefg	公农2号Gongnong No. 2	256.21±72.78cdefg	305.78±166.23bcdefg
Maverick	197.17±75.65fg	197.84±103.63defg	皇冠Phabulous	360.73±78.59bcdefg	341.11±105.01abcde
Orca	153.22±44.00fg	240.32±21.13cdefg	陇东Longdong	173.73±45.48fg	168.28±89.88fg
Ranger	506.51±79.07abcd	240.49±13.29cdefg	陇中Longzhong	383.56±133.14abcdef	161.55±34.42fg
三得利Sanditi	190.76±10.46fg	265.27±45.46cdefg	天水Tianshui	259.03±106.22cdefg	224.34±52.02cdefg
Saranac AR	283.33±63.41bcdefg	188.23±9.01efg	无棣Wudi	236.63±79.72defg	237.59±70.70cdefg
Sutter	340.65±88.37bcdefg	312.95±99.71bcdef	新疆大叶Xinjiang daye	424.21±206.9abcdef	378.21±60.32abc
Trifecta	522.56±45.09abcd	429.94±74.83ab	新牧2号Xinmu No. 2	329.43±154.57bcdefg	256.83±41.31cdefg
UC-1465	84.31±53.91g	150.97±57.17g	中兰1号Zhonglan No. 1	192.54±27.31fg	268.15±97.20cdefg
UC-1887	279.29±74.21bcdefg	201.96±41.10defg	中苜1号Zhongmu No. 1	240.05±66.29defg	167.90±52.59fg
Vernal	418.49±147.06abcdef	350.40±31.95abcd	中牧1号Zhongmu No. 1	249.72±166.26defg	351.74±31.64abcd
WL168HQ	298.74±156.44bcdefg	195.23±67.18defg	甘农1号Gannong No. 1	270.86±228.12cdefg	295.00±159.21bcdefg
WL319HQ	348.54±98.86bcdefg	222.56±31.19cdefg	甘农2号Gannong No. 2	199.08±18.78fg	235.80±26.59cdefg
WL343HQ	393.03±59.76abcdef	221.61±109.10cdefg	图牧1号Tumu No. 1	350.38±128.24bcdefg	260.49±52.36cdefg
WL354HQ	544.17±76.47abc	306.68±18.89bcdefg	阿尔冈金Algonquin	564.15±145.08ab	464.91±123.14a

排名Rank	品种 Germplasm	排名Rank	品种 Germplasm	排名Rank	品种 Germplasm	排名Rank	品种 Germplasm
1	阿尔冈金Algonquin	12	甘农1号Gannong No. 1	23	甘农4号Gannong No. 4	34	公农2号Gongnong No. 2
2	新疆大叶Xinjiang daye	13	新牧2号Xinmu No. 2	24	WL319HQ	35	UC-1887
3	Trifecta	14	Ranger	25	甘农6号Gannong No. 6	36	甘农5号Gannong No. 5
4	Vernal	15	德宝Derby	26	三得利Sanditi	37	Saranac AR
5	中牧1号Zhongmu No. 1	16	中兰1号Zhonglan No. 1	27	Arc	38	Maverick
6	皇冠Phabulous	17	Archer	28	Orca	39	中苜1号Zhongmu No. 1
7	Sutter	18	甘农2号Gannong No. 2	29	甘农7号Gannong No. 7	40	陇中Longzhong
8	WL354HQ	19	无棣Wudi	30	天水Tianshui	41	东苜1号Dongmu No. 1
9	Hunter River	20	WL363HQ	31	甘农3号Gannong No. 3	42	陇东Longdong
10	Boja	21	Abi 700	32	Aurora	43	CUF 101
11	图牧1号Tumu No. 1	22	WL343HQ	33	WL168HQ	44	UC-1465