Abstract: To explore the resource diversity and microbial inhibition activity of endophytic fungi from the medicinal plant Tamarix chinensis, endophytic fungi were isolated from root, stem, and leaf of the host by tissue inoculation culture. Five pathogenic fungi and four bacterial strains were used as indicators to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion. Eighteen endophytic fungal strains were isolated: most from the stems and least from the leaves. The isolated strains were divided into seven genera, three families, and two orders based on morphological characteristics. Among the isolates, eleven strains (61.1% of the total isolated) were found to have some microbial inhibition activities against two or more pathogenic fungi, eight strains (44.4%) had evident inhibition activity against Staphylococcus aureus with strain TCEFL001 showing evident antifungal and antibacterial activities against Colletotrichum gloeosporioides, Gaeumannomyces graminis var. tritici and S. aureus, belonging to Phacodium sp. The medicinal plant Tamarix chinensis is a good endophytic fungal resource, which is worth further study for its development and application.