Cloning and analysis of the phosphate transporter protein SgPT1 from Stylosanthes

doi:10.11686/cyxb20130423

Abstract

Abstract: Phosphorous (P) is one of the essential macronutrients and a high-affinity phosphate transporter (PT) plays an important role in P absorption and transport. SgPT1 encodes a high-affinity phosphate transporter from the roots of TPRC2001-1 which is a P efficient genotype. It was homologously cloned and a full length cDNA library was established under phosphorous deficiency conditions. The full length of SgPT1 is 1 994 bp, and it encodes 538 amino acids. The protein molecular weight of SgPT1 is 59 kD. The protein secondary structure is a 6+6 configuration, which is consistent with most high-affinity PT. The expression of SgPT1 was strongly enhanced by low phosphorous (LP) stress in stylo TPRC2001-1 root indicating that SgPT1 potentially participated in P uptake and transport under LP conditions. The increased expression of SgPT1 was one of the molecular mechanisms for style adaptation to LP stress and SgPT1 might be one of the candidate genes for clarifying the mechanisms.

CLC Number:

Q943.2

SUN Li-li, CHEN Zhi-jian, LIU Pan-dao, LIAO Hong, LIU Guo-dao, TIAN Jiang. Cloning and analysis of the phosphate transporter protein SgPT1 from Stylosanthes[J]. Acta Prataculturae Sinica, 2013, 22(4): 187-198.

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