Cloning and analysis of the Δ1-pyrroline-5-carboxylate synthetase (LcP5CS1)from Leymus chinensis

doi:10.11686/cyxb20130424

Abstract

Abstract: Leymus chinensis is tolerant to high salinity, drought and cold stresses. It is an important perennial plant with economic and ecological value. Proline is one of the main regulators in many plants under osmotic stresses. Δ1-pyrroline-5-carboxylate synthetase (P5CS) is a key regulatory enzyme that plays a crucial role in proline biosynthesis by the glutamate pathway. Based on analysis of solexa sequencing data, a P5CS gene from L. chinensis was cloned by RT-PCR using homologous primers. The cDNA sequence was 2 197 bp in length and putatively translated to 730 amino acids. Based on the sequence alignment results, the protein was named LcP5CS1. The homology between LcP5CS1 and BdP5CS1 was 95％, but LcP5CS1 had low homology with P5CS from dicotyledons. Analysis expression profiles of LcP5CS1 revealed that LcP5CS1 was highly expressed in leaf sheaths, stems and leaves. In cold conditions, ABA, salt and drought stress, it was significantly induced with time, and the expression levels of LcP5CS1 were up-regulated. The results of Solexa sequencing for wounded L. chinensis showed that LcP5CS1 was up-regulated, and the increased proline in L. chinensis may contribute to its stress tolerance.

CLC Number:

ZHANG Le-xin, SU Man, MA Tian, MA Xing-yong, YAN Xue-qing, PENG Xian-jun, CHEN Shuang-yan, CHENG Li-qin, LIU Gong-she. Cloning and analysis of the Δ1-pyrroline-5-carboxylate synthetase (LcP5CS1)from Leymus chinensis[J]. Acta Prataculturae Sinica, 2013, 22(4): 197-204.

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